LOCUS X04555 2235 bp DNA linear ENV 07-DEC-1995 DEFINITION IncC multiresistance plasmid pDGO100 aadB gene for AAD (2") aminoglycoside adenylyltransferase. ACCESSION X04555 VERSION X04555.1 KEYWORDS aadB gene; antibiotic resistance; gentamycin resistance; kanamycin resistance; tobramycin resistance. SOURCE uncultured bacterium ORGANISM uncultured bacterium Bacteria; environmental samples. REFERENCE 1 (bases 1 to 2235) AUTHORS Cameron F.H., Groot Obbink D.J., Ackerman V.P., Hall R.M. TITLE Nucleotide sequence of the AAD(2'') aminoglycoside adenylyltransferase determinant aadB. Evolutionary relationship of this region with those surrounding aadA in R538-1 and dhfrII in R388 JOURNAL Nucleic Acids Res. 14(21), 8625-8635(1986). PUBMED 3024112 REFERENCE 2 (bases 1 to 2235) AUTHORS Hall R.M. JOURNAL Submitted (04-AUG-1988) to the INSDC. COMMENT Data kindly reviewed (20-APR-1987) by D.J. Groot Obbink FEATURES Location/Qualifiers source 1..2235 /organism="uncultured bacterium" /plasmid="pDGO100" /environmental_sample /mol_type="genomic DNA" /db_xref="taxon:77133" old_sequence 294 /note="g was cg in [1]" /citation=[1] old_sequence 746 /note="a was ca in [1]" /citation=[1] regulatory 1035..1040 /note="pot. -35 region" /regulatory_class="promoter" regulatory 1058..1063 /note="pot. -10 region" /regulatory_class="promoter" regulatory 1071..1073 /note="pot. rRNA binding site" /regulatory_class="ribosome_binding_site" CDS 1080..1829 /transl_table=11 /note="AAD(2) (AA 1-249)" /db_xref="GOA:P0AE04" /db_xref="InterPro:IPR019646" /db_xref="UniProtKB/Swiss-Prot:P0AE04" /protein_id="CAA28209.1" /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFVQSMPRASKQQAR YAVGRCLMLWSSNDVTQQGSRPKTKLGRMDTTQVTLIHKILAAADERNLPLWIGGGWA IDARLGRVTRKHDDIDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCE PAWWADEAYEIAEAPQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPT KHIESYRLACTSLGAEKVEVLRAAFRSRYAA" regulatory 1139..1143 /note="alternative rRNA binding site" /regulatory_class="ribosome_binding_site" CDS 1149..1829 /transl_table=11 /note="alternative AAD(2) (AA 1-226)" /db_xref="GOA:Q51694" /db_xref="InterPro:IPR019646" /db_xref="UniProtKB/TrEMBL:Q51694" /protein_id="CAA28210.1" /translation="MACYDCFFVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPK TKLGRMDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDDIDLTFPGER RGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQGSCPEAA EGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLGAEKVEVLRA AFRSRYAA" misc_feature 1290..1825 /note="unique sequence (in comparison with the aadA gene region from R538-1)" misc_feature 1826..1880 /note="Tn7 54 base element" BASE COUNT 484 a 615 c 664 g 472 t ORIGIN 1 ggatccatca ggcaacgacg ggctgctgcc ggccatcagc ggacgcaggg aggactttcc 61 gcaaccggcc gttcgatgcg gcaccgatgg ccttcgcgca ggggtagtga atccgccagg 121 attgacttgc gctgccctac ctctcactag tgaggggcgg cagcgcatca agcggtgagc 181 gcactccggc accgccaact ttcagcacat gcgtgtaaat catcgtcgta gagacgtcgg 241 aatggccgag cagatcctgc acggttcgaa tgtcgtaacc gctgcggagc aaggccgtcg 301 cgaacgagtg gcggagggtg tgcggtgtgg cgggcttcgt gatgcctgct tgttctacgg 361 cacgtttgaa ggcgcgctga aaggtctggt catacatgtg atggcgacgc acgacaccgc 421 tccgtggatc ggtcgaatgc gtgtgctgcg caaaaaccca gaaccacggc caggaatgcc 481 cggcgcgcgg atacttccgc tcaagggcgt cgggaagcgc aacgccgctg cggccctcgg 541 cctggtcctt cagccaccat gcccgtgcac gcgacagctg ctcgcgcagg ctgggtgcca 601 agctctcggg taacatcaag gcccgatcct tggagccctt gccctcccgc acgatgatcg 661 tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa accctcactg atccgcatgc 721 ccgttccata cagaagctgg gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc 781 gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga ggtcttccga 841 tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg 901 ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc caggacagaa 961 atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga 1021 aggcacgaac ccagttgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta 1081 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg 1141 cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg gcatccaagc 1201 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc 1261 agcagggcag tcgccctaaa acaaagttag gccgcatgga cacaacgcag gtcacattga 1321 tacacaaaat tctagctgcg gcagatgagc gaaatctgcc gctctggatc ggtgggggct 1381 gggcgatcga tgcacggcta gggcgtgtaa cacgcaagca cgatgatatt gatctgacgt 1441 ttcccggcga gaggcgcggc gagctcgagg caatagttga aatgctcggc gggcgcgtca 1501 tggaggagtt ggactatgga ttcttagcgg agatcgggga tgagttactt gactgcgaac 1561 ctgcttggtg ggcagacgaa gcgtatgaaa tcgcggaggc tccgcagggc tcgtgcccag 1621 aggcggctga gggcgtcatc gccgggcggc cagtccgttg taacagctgg gaggcgatca 1681 tctgggatta cttttactat gccgatgaag taccaccagt ggactggcct acaaagcaca 1741 tagagtccta caggctcgca tgcacctcac tcggggcgga aaaggttgag gtcttgcgtg 1801 ccgctttcag gtcgcgatat gcggcctaac aattcgtcca agccgacgcc gcttcgcggc 1861 gcggcttaac tcaggtgtta gatgcactaa gcacataatt gctcacagcc aaactatcag 1921 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat 1981 atatcatgaa aggctggctt tttcttgtta tcgcaatagt tggcgaagta atcgcaacat 2041 ccgcattaaa atctagcgag ggctttgcta agcttgcccc ttccgccgtt gtcataatcg 2101 gttatggcga tcgcatttta ttttctttct ctggttctga aatccatccc tgtcggtgtt 2161 gcttatgcag tctggtcggg actcggcgtc gtcataatta cagccattgc ctggttgctt 2221 catgggcaaa agctt //