LOCUS       X04555                  2235 bp    DNA     linear   ENV 07-DEC-1995
DEFINITION  IncC multiresistance plasmid pDGO100 aadB gene for AAD (2")
            aminoglycoside adenylyltransferase.
ACCESSION   X04555
VERSION     X04555.1
KEYWORDS    aadB gene; antibiotic resistance; gentamycin resistance; kanamycin
            resistance; tobramycin resistance.
SOURCE      uncultured bacterium
  ORGANISM  uncultured bacterium
            Bacteria; environmental samples.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Cameron F.H., Groot Obbink D.J., Ackerman V.P., Hall R.M.
  TITLE     Nucleotide sequence of the AAD(2'') aminoglycoside
            adenylyltransferase determinant aadB. Evolutionary relationship of
            this region with those surrounding aadA in R538-1 and dhfrII in
            R388
  JOURNAL   Nucleic Acids Res. 14(21), 8625-8635(1986).
   PUBMED   3024112
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Hall R.M.
  JOURNAL   Submitted (04-AUG-1988) to the INSDC.
COMMENT     Data kindly reviewed (20-APR-1987) by D.J. Groot Obbink
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="uncultured bacterium"
                     /plasmid="pDGO100"
                     /environmental_sample
                     /mol_type="genomic DNA"
                     /db_xref="taxon:77133"
     old_sequence    294
                     /note="g was cg in [1]"
                     /citation=[1]
     old_sequence    746
                     /note="a was ca in [1]"
                     /citation=[1]
     regulatory      1035..1040
                     /note="pot. -35 region"
                     /regulatory_class="promoter"
     regulatory      1058..1063
                     /note="pot. -10 region"
                     /regulatory_class="promoter"
     regulatory      1071..1073
                     /note="pot. rRNA binding site"
                     /regulatory_class="ribosome_binding_site"
     CDS             1080..1829
                     /transl_table=11
                     /note="AAD(2) (AA 1-249)"
                     /db_xref="GOA:P0AE04"
                     /db_xref="InterPro:IPR019646"
                     /db_xref="UniProtKB/Swiss-Prot:P0AE04"
                     /protein_id="CAA28209.1"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLGRMDTTQVTLIHKILAAADERNLPLWIGGGWA
                     IDARLGRVTRKHDDIDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCE
                     PAWWADEAYEIAEAPQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPT
                     KHIESYRLACTSLGAEKVEVLRAAFRSRYAA"
     regulatory      1139..1143
                     /note="alternative rRNA binding site"
                     /regulatory_class="ribosome_binding_site"
     CDS             1149..1829
                     /transl_table=11
                     /note="alternative AAD(2) (AA 1-226)"
                     /db_xref="GOA:Q51694"
                     /db_xref="InterPro:IPR019646"
                     /db_xref="UniProtKB/TrEMBL:Q51694"
                     /protein_id="CAA28210.1"
                     /translation="MACYDCFFVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPK
                     TKLGRMDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDDIDLTFPGER
                     RGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQGSCPEAA
                     EGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLGAEKVEVLRA
                     AFRSRYAA"
     misc_feature    1290..1825
                     /note="unique sequence (in comparison with the aadA gene
                     region from R538-1)"
     misc_feature    1826..1880
                     /note="Tn7 54 base element"
BASE COUNT          484 a          615 c          664 g          472 t
ORIGIN      
        1 ggatccatca ggcaacgacg ggctgctgcc ggccatcagc ggacgcaggg aggactttcc
       61 gcaaccggcc gttcgatgcg gcaccgatgg ccttcgcgca ggggtagtga atccgccagg
      121 attgacttgc gctgccctac ctctcactag tgaggggcgg cagcgcatca agcggtgagc
      181 gcactccggc accgccaact ttcagcacat gcgtgtaaat catcgtcgta gagacgtcgg
      241 aatggccgag cagatcctgc acggttcgaa tgtcgtaacc gctgcggagc aaggccgtcg
      301 cgaacgagtg gcggagggtg tgcggtgtgg cgggcttcgt gatgcctgct tgttctacgg
      361 cacgtttgaa ggcgcgctga aaggtctggt catacatgtg atggcgacgc acgacaccgc
      421 tccgtggatc ggtcgaatgc gtgtgctgcg caaaaaccca gaaccacggc caggaatgcc
      481 cggcgcgcgg atacttccgc tcaagggcgt cgggaagcgc aacgccgctg cggccctcgg
      541 cctggtcctt cagccaccat gcccgtgcac gcgacagctg ctcgcgcagg ctgggtgcca
      601 agctctcggg taacatcaag gcccgatcct tggagccctt gccctcccgc acgatgatcg
      661 tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa accctcactg atccgcatgc
      721 ccgttccata cagaagctgg gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc
      781 gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga ggtcttccga
      841 tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg
      901 ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc caggacagaa
      961 atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     1021 aggcacgaac ccagttgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta
     1081 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     1141 cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg gcatccaagc
     1201 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1261 agcagggcag tcgccctaaa acaaagttag gccgcatgga cacaacgcag gtcacattga
     1321 tacacaaaat tctagctgcg gcagatgagc gaaatctgcc gctctggatc ggtgggggct
     1381 gggcgatcga tgcacggcta gggcgtgtaa cacgcaagca cgatgatatt gatctgacgt
     1441 ttcccggcga gaggcgcggc gagctcgagg caatagttga aatgctcggc gggcgcgtca
     1501 tggaggagtt ggactatgga ttcttagcgg agatcgggga tgagttactt gactgcgaac
     1561 ctgcttggtg ggcagacgaa gcgtatgaaa tcgcggaggc tccgcagggc tcgtgcccag
     1621 aggcggctga gggcgtcatc gccgggcggc cagtccgttg taacagctgg gaggcgatca
     1681 tctgggatta cttttactat gccgatgaag taccaccagt ggactggcct acaaagcaca
     1741 tagagtccta caggctcgca tgcacctcac tcggggcgga aaaggttgag gtcttgcgtg
     1801 ccgctttcag gtcgcgatat gcggcctaac aattcgtcca agccgacgcc gcttcgcggc
     1861 gcggcttaac tcaggtgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     1921 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     1981 atatcatgaa aggctggctt tttcttgtta tcgcaatagt tggcgaagta atcgcaacat
     2041 ccgcattaaa atctagcgag ggctttgcta agcttgcccc ttccgccgtt gtcataatcg
     2101 gttatggcga tcgcatttta ttttctttct ctggttctga aatccatccc tgtcggtgtt
     2161 gcttatgcag tctggtcggg actcggcgtc gtcataatta cagccattgc ctggttgctt
     2221 catgggcaaa agctt
//