LOCUS X00006 2709 bp DNA linear ENV 12-JUN-2006
DEFINITION E.coli plasmid RP1 tetracycline resistance (tet) determinants:
regulatory gene tetR and resistance gene tetA.
ACCESSION X00006
VERSION X00006.1
KEYWORDS plasmid; regulatory protein; tetracycline resistance.
SOURCE uncultured bacterium
ORGANISM uncultured bacterium
Bacteria; environmental samples.
REFERENCE 1 (bases 1 to 2709)
AUTHORS Waters S.H., Rogowsky P., Grinsted J., Altenbuchner J., Schmitt R.
TITLE The tetracycline resistance determinants of RP1 and Tn1721:
nucleotide sequence analysis
JOURNAL Nucleic Acids Res. 11(17), 6089-6105(1983).
PUBMED 6310527
FEATURES Location/Qualifiers
source 1..2709
/organism="uncultured bacterium"
/plasmid="RP1"
/environmental_sample
/mol_type="genomic DNA"
/db_xref="taxon:77133"
variation 318..318
/note="C is U in transposon Tn1721"
CDS complement(572..1222)
/transl_table=11
/note="tetR regulatory protein"
/db_xref="GOA:P03038"
/db_xref="InterPro:IPR001647"
/db_xref="InterPro:IPR003012"
/db_xref="InterPro:IPR004111"
/db_xref="InterPro:IPR009057"
/db_xref="InterPro:IPR023772"
/db_xref="InterPro:IPR036271"
/db_xref="PDB:5MRU"
/db_xref="UniProtKB/Swiss-Prot:P03038"
/protein_id="CAA24908.1"
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
FRNKRALLDALAEAMLAENHSTSVPRADDDWRSFLTGNARSFRQALLAYRDGARIHAG
TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSESGERGG
TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
misc_feature complement(1222..1222)
/note="putative primary transcript (tetR1)"
regulatory 1225..1246
/note="-35 region, tetA"
/regulatory_class="promoter"
regulatory complement(1230..1235)
/note="-10 region, tetR1"
/regulatory_class="promoter"
regulatory 1232..1235
/note="put. palindromic operator sequence O(L) left"
/regulatory_class="other"
misc_feature complement(1241..1241)
/note="putative secondary transcript (tetR2)"
regulatory complement(1248..1253)
/note="putative secondary promoter -10 region, tetR2"
/regulatory_class="promoter"
regulatory 1249..1254
/note="-10 region, tetA"
/regulatory_class="promoter"
regulatory complement(1254..1259)
/note="-35 region, tetR1"
/regulatory_class="promoter"
regulatory 1265..1277
/note="put.palindromic operator sequence O(R) right
binding tet repressor"
/regulatory_class="other"
regulatory complement(1272..1277)
/note="putative secondary promoter -35 region, tetR2"
/regulatory_class="promoter"
CDS 1328..2527
/transl_table=11
/note="possible tetA protein"
/db_xref="GOA:P02982"
/db_xref="InterPro:IPR001958"
/db_xref="InterPro:IPR004734"
/db_xref="InterPro:IPR005829"
/db_xref="InterPro:IPR011701"
/db_xref="InterPro:IPR020846"
/db_xref="InterPro:IPR036259"
/db_xref="UniProtKB/Swiss-Prot:P02982"
/protein_id="CAA24909.1"
/translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
YGILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIG
RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLSFVRWARGMTVVAALMAV
FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
AGQRADR"
variation 1341..1341
/note="U is G in transposon Tn1721"
variation 1490..1490
/note="G is A in transposon Tn1721"
variation 1549..1550
/note="AA is GG in transposon Tn1721"
variation 1576..1577
/note="CA is UG in transposon Tn1721"
BASE COUNT 468 a 861 c 858 g 522 t
ORIGIN
1 ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac ggccgccagc
61 ccagcggcga gggcaaccag cccggtgagc gtcggaaagg cgctggaagc cccgtagcga
121 cgcggagagg ggcgagacaa gccaagggcg caggctcgag tcgcagcacg acatagccgg
181 ttctcgcaag gacgagaatt tccctgcggt gcccctcaag tgtcaatgaa agtttccaac
241 gcgagccatt cgcgagagcc ttgagtccac gctagatcta tctcatctgc gcaaggcaga
301 acgtgaagac ggccgccctg gacctcgccc gcgagcgcca gcgcacgagg ccggcgcgcg
361 gacccgccgc ggcccacgag cggacgccgc agcaggagcg ccagaaggcc gccagagagg
421 ccgagcgcgg cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggcgc
481 tacggggctc tgacgcggtg gaaaggggga ggggatgttg tctacatggc tctgctgtag
541 tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc accctttctc ggtccttcaa
601 cgttcctgac aacgagcctc cttttcgcca atccatcgac aatcaccgcg agtccctgct
661 cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg
721 agagcggagc ctgttcaacg gtgccgccgc gctcgccgga ctcgctgtcg ccggcctgct
781 cctcaagcac ggccccaaca gtgaagtagc tgattgtcat cagcgcattg acggcgtccc
841 cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg
901 gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct
961 gcctgaagct gcgggcattc ccagtcagaa atgagcgcca gtcgtcgtcg gctctcggca
1021 ccgaagtgct atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct
1081 tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc
1141 gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc tagggcggca cggatcactg
1201 tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac
1261 caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag
1321 gccagacgtg aaacccaaca tacccctgat cgtaattctg agcactgtcg cgctcgacgc
1381 tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc
1441 gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttgg tgcaatttgc
1501 ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt
1561 ctcgctggcc ggcgccactg tcgactacgc catcatggcg acagcgcctt tcctttgggt
1621 tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc
1681 ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc
1741 ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc
1801 cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg
1861 tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa
1921 cccgctcagc ttcgttcggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt
1981 cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga
2041 ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct
2101 gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg
2161 ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac
2221 acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc
2281 ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgccaggggc agctgcaagg
2341 ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat
2401 ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta
2461 cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga
2521 tcgctgatcg tggaaacgat aggcctatgc catgcgggtc aaggcgactt ccggcaagct
2581 atacgcgccc taggagtgcg gttggaacgt tggcccagcc agacactccc gatcacgagc
2641 aggacgccga tgatttgaag cgcactcagc gtctgatcca agaacaacca tcctagcaac
2701 agcgcggcg
//