LOCUS       LC461025                1526 bp    DNA     linear   ROD 21-NOV-2019
DEFINITION  Mus musculus C57BL/6 DNA, contains a forebrain specific long-range
            enhancer of sonic hedgehog (Shh) gene.
ACCESSION   LC461025
VERSION     LC461025.1
KEYWORDS    .
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Sagai,T. and Shiroishi,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2019) to the DDBJ/EMBL/GenBank databases.
            Contact:Tomoko Sagai
            National Institute of Genetics, Mammalian Genetics laboratory;
            1111 Yata, Mishima, Shizuoka 411-8540, Japan
REFERENCE   2  
  AUTHORS   Sagai,T., Amano,T., Maeno,A., Ajima,R. and Shiroishi,T.
  TITLE     SHH signaling mediated by a prechordal and brain enhancer controls
            forebrain organization
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 116, 23636-23642 (2019)
  REMARK    DOI:10.1073/pnas.1901732116
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1526
                     /chromosome="5"
                     /db_xref="taxon:10090"
                     /dev_stage="adult"
                     /mol_type="genomic DNA"
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /tissue_type="skin"
     regulatory      343..1200
                     /experiment="This enhancer drives the transgenic reporter
                     expression in the axial mesoderm and neuroepithelium of
                     the forebrain. Deletion of this enhancer from mouse
                     genome causes reduction of the Shh expression in the
                     prechordal plate and the forebrain midline. As the
                     results, the knockout mice cause the craniofacial defects
                     similar with human holoprosencephaly (HPE)"
                     /gene="Shh"
                     /note="SBE7(e2)"
                     /note="contains a forebrain specific long-range enhancer
                     of sonic hedgehog (Shh) gene."
                     /regulatory_class="enhancer"
BASE COUNT          436 a          372 c          364 g          354 t
ORIGIN      
        1 gtaaggttcc acaagatacc atcccgagtt ttcactcaag gccacactgt tgcctaatga
       61 aagagctcac cccaaagctg cccttttact gccttctctg tgaaggtttg tcctggctgc
      121 aatcccagga ccagcagcag tttgcagagt tttcaagata attcactatc tataccacag
      181 tgaccaacac agttctttac tacagagctg cctagtctgc tggccgtgga gaataagatt
      241 cagaacatgg tgtttgtcca cctgaaggct cacctcagaa ctctgctctg cagacactgc
      301 tctgtcaggt tccaacagag tgctctgggg gggggggggg gagtgtcact gcattgaagg
      361 tgtcctgttg taaagttaat ttccagcagt gagtctacag cttcatgcta gagcaaagat
      421 ctggacaaag ccaaatttcc agaagccacc aagggctcca ggtactgctg ttcagcattc
      481 ccagcggtgt cttatgaaaa cacgcagcag cttagcaggg cacttagttt gtaaagaggc
      541 ttgtctctgc agcctatgtc tgcaataaag aaaaaaaaag cattcaacat ctcaggatta
      601 caagagcaac aattaaggct gggcctgaaa gctgacctcc tcctcagagg ccatgtgctc
      661 ttgcttgggc tgaagaaaac tatgtcagac ttaggtgctg gtttttacat aagccccaat
      721 ctccctttag caaacaaaag ctaaagaaaa atcccagggc ctccctggac tggggaagaa
      781 cacaaacagg gaggtggggg aagggcacat gatgaaagcg gggagggagg gagggaccca
      841 cccctcactc ctcagcctag atccctccaa cccctttcag gctgaaaagg aaaacatgaa
      901 gctttaccac acaatccctt cggtcaactt agccaggatg gcactttggg gactcctttc
      961 acacagtcct gtgagaggcc taacttgaaa gtgttcttta atgtcttgaa agcagaaagc
     1021 aaattggaga aatagttgca gacactttga tgggcctggg agctgctctg gcctctcagg
     1081 acaaggtgca gtcataggaa gaaaagcctt ttgtggctaa gcggcctctc taagccaatg
     1141 cttgccatcc acatgtcaga tcacagggga aatggaaact catggaagac ttgtcgcctt
     1201 ctagagctgg tttgtttttg actgcccaca gcaattaaca ggaaggggga aggcctcctt
     1261 catctgtcta accagagaaa aacaacaacg aataatccca ccattgataa aacccacgct
     1321 ttagaatggc ttcctcttgt cactagccta gtgtgcttta tgtgttctta aactaagaat
     1381 ggccagaata aggaatagaa atgtgaatgc cctcacagtc acagaagtaa gcgaaggagc
     1441 aggtttctga gacactaagc caaacagagc tgcatgacgt ccttcatctc acaaggcatg
     1501 actcacgcct ctgaagagac acatgg
//