LOCUS HS080288 457 bp mRNA linear EST 15-JUL-2011 DEFINITION nta0001 Tobacco cultivar SR1 apical cell cDNA library Nicotiana tabacum cDNA, mRNA sequence. ACCESSION HS080288 VERSION HS080288.1 DBLINK BioSample: SAMN00169851 KEYWORDS EST. SOURCE Nicotiana tabacum (common tobacco) ORGANISM Nicotiana tabacum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana. REFERENCE 1 (bases 1 to 457) AUTHORS Zhao,J., Xin,H., Qu,L., Ning,J., Peng,X., Yan,T., Ma,L., Li,S. and Sun,M.X. TITLE Dynamic changes of transcript profiles after fertilization are associated with de novo transcription and maternal elimination in tobacco zygote, and mark the onset of the maternal-to-zygotic transition JOURNAL Plant J. 65 (1), 131-145 (2011) PUBMED 21175896 COMMENT Contact: Mengxiang Sun Key Laboratory of the Ministry of Education for Plant Developmental Biology College of Life Science, Wuhan University Box 3012, Hubei, China 430072 Tel: 0086-27-68756170(O) Fax: 0086-27-68756010 Email: mxsun@whu.edu.cn Seq primer: pTripEX2 5' sequencing primer. FEATURES Location/Qualifiers source 1..457 /organism="Nicotiana tabacum" /mol_type="mRNA" /cultivar="SR1" /db_xref="taxon:4097" /cell_type="apical cell of two-celled proembryo" /clone_lib="SAMN00169851 Tobacco cultivar SR1 apical cell cDNA library" /dev_stage="after first zygote division" /lab_host="XL1-blue" /note="Vector: pTripEX2; Site_1: Sfi IA; Site_2: Sfi IB; Two-celled proembryo were manually collected from ovules. An LMD (Leica, Bonn, Germany) was used to destroy basal cell of two-celled proembryos to obtain the apical cell. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2." BASE COUNT 127 a 76 c 121 g 132 t ORIGIN 1 gggacaaaaa aacaaaagca gaaatggcga agcgtttgct cccaacactc aacagggttt 61 tgatcgagaa aatcaccgcc cctgnctaaa actaatgccg gtattcttct ccttgtaaaa 121 gtcctctaag ctgaattccg gtaaggtggt ggctgttggg ccagggctgc gtgacaagtc 181 aggaaacttg attcccactg ctgtcaagga aggtgacact gtcttgttgc ctgagtatgg 241 tggtacacaa gtcaagttgg gggagaaaga gtttcatttt accgggatga ggacattttg 301 gggacgcttc atgactgaga tcatctatat atttgagttc attcaaacct tgaggttaaa 361 cttgagggga agtggggttt attttgggat gttttggttg aatattggaa ttgttgaagt 421 gtttttaatc tctaatgcag tgatagttga aacaaaa //