LOCUS       HS080288                 457 bp    mRNA    linear   EST 15-JUL-2011
DEFINITION  nta0001 Tobacco cultivar SR1 apical cell cDNA library Nicotiana
            tabacum cDNA, mRNA sequence.
ACCESSION   HS080288
VERSION     HS080288.1
DBLINK      BioSample: SAMN00169851
KEYWORDS    EST.
SOURCE      Nicotiana tabacum (common tobacco)
  ORGANISM  Nicotiana tabacum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; asterids; lamiids; Solanales; Solanaceae;
            Nicotianoideae; Nicotianeae; Nicotiana.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Zhao,J., Xin,H., Qu,L., Ning,J., Peng,X., Yan,T., Ma,L., Li,S. and
            Sun,M.X.
  TITLE     Dynamic changes of transcript profiles after fertilization are
            associated with de novo transcription and maternal elimination in
            tobacco zygote, and mark the onset of the maternal-to-zygotic
            transition
  JOURNAL   Plant J. 65 (1), 131-145 (2011)
   PUBMED   21175896
COMMENT     Contact: Mengxiang Sun
            Key Laboratory of the Ministry of Education for Plant Developmental
            Biology
            College of Life Science, Wuhan University
            Box 3012, Hubei, China 430072
            Tel: 0086-27-68756170(O)
            Fax: 0086-27-68756010
            Email: mxsun@whu.edu.cn
            Seq primer: pTripEX2 5' sequencing primer.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Nicotiana tabacum"
                     /mol_type="mRNA"
                     /cultivar="SR1"
                     /db_xref="taxon:4097"
                     /cell_type="apical cell of two-celled proembryo"
                     /clone_lib="SAMN00169851 Tobacco cultivar SR1 apical cell
                     cDNA library"
                     /dev_stage="after first zygote division"
                     /lab_host="XL1-blue"
                     /note="Vector: pTripEX2; Site_1: Sfi IA; Site_2: Sfi IB;
                     Two-celled proembryo were manually collected from ovules.
                     An LMD (Leica, Bonn, Germany) was used to destroy basal
                     cell of two-celled proembryos to obtain the apical cell.
                     RNA was isolated and cDNA library was constructed by using
                     the Clontech SMART cDNA library construction kit. The
                     cDNAs were directionally ligated into pTripEX2."
BASE COUNT          127 a           76 c          121 g          132 t
ORIGIN      
        1 gggacaaaaa aacaaaagca gaaatggcga agcgtttgct cccaacactc aacagggttt
       61 tgatcgagaa aatcaccgcc cctgnctaaa actaatgccg gtattcttct ccttgtaaaa
      121 gtcctctaag ctgaattccg gtaaggtggt ggctgttggg ccagggctgc gtgacaagtc
      181 aggaaacttg attcccactg ctgtcaagga aggtgacact gtcttgttgc ctgagtatgg
      241 tggtacacaa gtcaagttgg gggagaaaga gtttcatttt accgggatga ggacattttg
      301 gggacgcttc atgactgaga tcatctatat atttgagttc attcaaacct tgaggttaaa
      361 cttgagggga agtggggttt attttgggat gttttggttg aatattggaa ttgttgaagt
      421 gtttttaatc tctaatgcag tgatagttga aacaaaa
//