LOCUS HS003764 743 bp mRNA linear EST 26-OCT-2010
DEFINITION CCXA1000.b1 CCXA Euonymus alatus seed tissues collected from 8/14
to 9/19/2007 normalized Euonymus alatus cDNA clone CCXA1000 5',
mRNA sequence.
ACCESSION HS003764
VERSION HS003764.1
DBLINK BioSample: SAMN00169828
KEYWORDS EST.
SOURCE Euonymus alatus
ORGANISM Euonymus alatus
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
Pentapetalae; rosids; fabids; Celastrales; Celastraceae; Euonymus.
REFERENCE 1 (bases 1 to 743)
AUTHORS Lucas,S., Rokhsar,D., Wang,M., Lindquist,E.A., Durrett,T.P.,
Ohlrogge,J. and Pollard,M.
TITLE DOE Joint Genome Institute Euonymus alatus EST project
JOURNAL Unpublished
COMMENT Contact: Lindquist,E.A.
DOE Joint Genome Institute
2800 Mitchell Drive, Walnut Creek, CA 94598, USA
Tel: 925 296 5600
Fax: 925 296 5710
Email: cdna@jgi-psf.org
Tissue Procurement: Ohlrogge lab, Michigan State University cDNA
Library Preparation: DOE Joint Genome Institute:
http://www.jgi.doe.gov cDNA Library Arrayed by: DOE Joint Genome
Institute: http://www.jgi.doe.gov DNA Sequencing: DOE Joint Genome
Institute http://www.jgi.doe.gov Clone Distribution: For inquiries
regarding access to clones: John Ohlrogge (ohlrogge@msu.edu)
Plate: CCXA 0009 row: O column: 10.
FEATURES Location/Qualifiers
source 1..743
/organism="Euonymus alatus"
/mol_type="mRNA"
/db_xref="taxon:4307"
/clone="CCXA1000"
/clone_lib="SAMN00169828 CCXA Euonymus alatus seed tissues
collected from 8/14 to 9/19/2007 normalized"
/lab_host="DH10B"
/note="Vector: pDNR-LIB; Site_1: SfiIA; Site_2: SfiIB; The
library was made from oligo(dT)-primed cDNA and cloned
into vector pDNR-LIB (Clontech Laboratories, Inc.,
Mountain View, CA) using the SMART system (BD Biosciences,
San Jose, CA). To generate the first strand, polyA RNA was
primed with an oligo(dT) primer
(5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A,
G, or C). The second strand was synthesized by PCR using
primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'
followed by digestion of the SfiI sites. cDNA was size
selected from 2-8kb using 1.1% agarose gel
electrophoresis, then ligated into pDNR-LIB. Normalized
library was created and sequencing performed by the DOE
Joint Genome Institute (Walnut Creek, CA)."
BASE COUNT 189 a 146 c 193 g 215 t
ORIGIN
1 gacaaacgcg ttggtgtcaa gctttgtctc tgtttgcgct tcgcaaattc tctgagaagc
61 tcttgaaaga gggactaata gtggtaagga gaaatggggc tgacatttac caagctcttc
121 agccggctct ttgccaagaa agagatgcgc attctgatgg tgggtcttga tgctgctggt
181 aagacaacca tattgtacaa gctaaagctt ggtgagatcg tcaccactat cccaactatt
241 gggttcaatg tggaaactgt ggaatacaaa aacattagtt tcactgtgtg ggatgttggg
301 ggtcaggaca agatccgtcc cttgtggagg cactacttcc aaaacacaca gggcctcatt
361 tttgtcgtag acagcaatga cagagaccga gttgttgagg ctagggatga gttgcacagg
421 atgttgaatg aggatgagct gcgagatgca gttttgctcg tatttgccaa caaacaggat
481 ctgcctaatg cgatgaatgc tgctgagatt actgataagc ttgcgctcaa ctccctccgt
541 cagcgtcact ggtacatcca gagcacttgt gcaacctctg gagaggggct ttatgagggt
601 ttagattggc tatccaacaa cattgccaac aaggcgtaaa tctgttggga aaacgattat
661 ggactggcaa ttctggatgc aggggtgaat attatctagt gttttttctt ccttacattt
721 tttgttttgt tttgtttctc tca
//