LOCUS HO867982 338 bp mRNA linear EST 25-OCT-2010 DEFINITION CFNX1000.b1 CFNX Botryococcus braunii UTEX 572 normalized (H) Botryococcus braunii cDNA clone CFNX1000 5', mRNA sequence. ACCESSION HO867982 VERSION HO867982.1 DBLINK BioSample: SAMN00169823 KEYWORDS EST. SOURCE Botryococcus braunii ORGANISM Botryococcus braunii Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae; Trebouxiophyceae incertae sedis; Elliptochloris clade; Botryococcus. REFERENCE 1 (bases 1 to 338) AUTHORS Hadi,M., Lucas,S., Rokhsar,D., Wang,M., Lindquist,E.A. and Beller,H. TITLE DOE Joint Genome Institute Botryococcus braunii UTEX 572 EST project JOURNAL Unpublished COMMENT Contact: Lindquist,E.A. DOE Joint Genome Institute 2800 Mitchell Drive, Walnut Creek, CA 94598, USA Tel: 925 296 5600 Fax: 925 296 5710 Email: cdna@jgi-psf.org Tissue Procurement: Laboratory of H. Beller cDNA Library Preparation: DOE Joint Genome Institute: http://www.jgi.doe.gov cDNA Library Arrayed by: DOE Joint Genome Institute: http://www.jgi.doe.gov DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov Clone Distribution: For inquiries regarding access to clones: H. Beller (HRBeller@lbl.gov) Plate: CFNX 0009 row: O column: 10. FEATURES Location/Qualifiers source 1..338 /organism="Botryococcus braunii" /mol_type="mRNA" /strain="UTEX 572" /db_xref="taxon:38881" /clone="CFNX1000" /clone_lib="SAMN00169823 CFNX Botryococcus braunii UTEX 572 normalized (H)" /lab_host="DH10B" /note="Vector: pDNR-LIB; Site_1: SfiIA; Site_2: SfiIB; The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected then ligated into pDNR-LIB. Library is normalized and is size-selected for inserts >1 kb. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).'" BASE COUNT 83 a 67 c 108 g 80 t ORIGIN 1 atgcaaatgc ggatggactt tgcttccgag tgggaggact acccgatgca ggaggctgag 61 ggggcttgga acatgttgat acagccgaat gtcgtcaagg ctctggaagg cgttttcaag 121 aggctggctg ggaagaagca agcgaagttg tagtttaacg aatgaggagc agcagcgtcc 181 ttgcaggacc atgtccactg ctggctagtg tggtttgtat agacaggagg ctgtggcaca 241 tagccttcac caaggattgc tttgtgttcc cttcatatgg tgtgaggaca tggcgcatac 301 ttgacgtcag tacaaatcac atgaagtgct gcaggaca //