LOCUS       HO867982                 338 bp    mRNA    linear   EST 25-OCT-2010
DEFINITION  CFNX1000.b1 CFNX Botryococcus braunii UTEX 572 normalized (H)
            Botryococcus braunii cDNA clone CFNX1000 5', mRNA sequence.
ACCESSION   HO867982
VERSION     HO867982.1
DBLINK      BioSample: SAMN00169823
KEYWORDS    EST.
SOURCE      Botryococcus braunii
  ORGANISM  Botryococcus braunii
            Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes;
            Trebouxiophyceae; Trebouxiophyceae incertae sedis; Elliptochloris
            clade; Botryococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Hadi,M., Lucas,S., Rokhsar,D., Wang,M., Lindquist,E.A. and
            Beller,H.
  TITLE     DOE Joint Genome Institute Botryococcus braunii UTEX 572 EST
            project
  JOURNAL   Unpublished
COMMENT     Contact: Lindquist,E.A.
            DOE Joint Genome Institute
            2800 Mitchell Drive, Walnut Creek, CA 94598, USA
            Tel: 925 296 5600
            Fax: 925 296 5710
            Email: cdna@jgi-psf.org
            Tissue Procurement: Laboratory of H. Beller cDNA Library
            Preparation: DOE Joint Genome Institute: http://www.jgi.doe.gov
            cDNA Library Arrayed by: DOE Joint Genome Institute:
            http://www.jgi.doe.gov DNA Sequencing: DOE Joint Genome Institute:
            http://www.jgi.doe.gov Clone Distribution: For inquiries regarding
            access to clones: H. Beller (HRBeller@lbl.gov)
            Plate: CFNX 0009  row: O  column: 10.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Botryococcus braunii"
                     /mol_type="mRNA"
                     /strain="UTEX 572"
                     /db_xref="taxon:38881"
                     /clone="CFNX1000"
                     /clone_lib="SAMN00169823 CFNX Botryococcus braunii UTEX
                     572 normalized (H)"
                     /lab_host="DH10B"
                     /note="Vector: pDNR-LIB; Site_1: SfiIA; Site_2: SfiIB; The
                     library was made from oligo(dT)-primed cDNA and cloned
                     into vector pDNR-LIB (Clontech Laboratories, Inc.,
                     Mountain View, CA) using the SMART system (BD Biosciences,
                     San Jose, CA). To generate the first strand, polyA RNA was
                     primed with an oligo(dT) primer
                     (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A,
                     G, or C). The second strand was synthesized by PCR using
                     primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'
                     followed by digestion of the SfiI sites. cDNA was size
                     selected then ligated into pDNR-LIB. Library is normalized
                     and is size-selected for inserts >1 kb. Library was
                     created and sequencing done by the DOE Joint Genome
                     Institute (Walnut Creek, CA).'"
BASE COUNT           83 a           67 c          108 g           80 t
ORIGIN      
        1 atgcaaatgc ggatggactt tgcttccgag tgggaggact acccgatgca ggaggctgag
       61 ggggcttgga acatgttgat acagccgaat gtcgtcaagg ctctggaagg cgttttcaag
      121 aggctggctg ggaagaagca agcgaagttg tagtttaacg aatgaggagc agcagcgtcc
      181 ttgcaggacc atgtccactg ctggctagtg tggtttgtat agacaggagg ctgtggcaca
      241 tagccttcac caaggattgc tttgtgttcc cttcatatgg tgtgaggaca tggcgcatac
      301 ttgacgtcag tacaaatcac atgaagtgct gcaggaca
//