LOCUS HO864409 795 bp mRNA linear EST 25-OCT-2010 DEFINITION JSPRRC001_01-A01-T3 Chromera velia CvLp_vc08/1 cDNA library Chromera velia cDNA, mRNA sequence. ACCESSION HO864409 VERSION HO864409.1 DBLINK BioSample: SAMN00169820 KEYWORDS EST. SOURCE Chromera velia ORGANISM Chromera velia Eukaryota; Sar; Alveolata; Colpodellida; Chromeraceae; Chromera. REFERENCE 1 (bases 1 to 795) AUTHORS Slapeta,J. and Carter,D. TITLE Expression sequence tags of Chromera velia JOURNAL Unpublished COMMENT Contact: Jan Slapeta Laboratory of Veterinary Parasitology University of Sydney McMaster Bld. B14, Faculty of Veterinary Science, Sydney, Australia Tel: 61 2 935 12025 Fax: 61 2 9351 7348 Email: jan.slapeta@sydney.edu.au PCR PRimers FORWARD: M13-20for; T7 BACKWARD: T3; M13rev. FEATURES Location/Qualifiers source 1..795 /organism="Chromera velia" /mol_type="mRNA" /strain="CvLp_vc08/1" /db_xref="taxon:505693" /clone_lib="SAMN00169820 Chromera velia CvLp_vc08/1 cDNA library" /note="Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; Chromera velia isolated from stony coral Leptastrea purpurea from One Tree Island, Australia. Cells were grown in f/2 medium and salinity 40 g.L-1; incubated at 25 C under 12:12 hours light:dark cycle with light intensity of 165 umol.m-2.s-1; 3-4 weeks old. Cell pellets were snap frozen in liquid N2, ground in liquid N2 until a fine powder and total RNA isolated using Ribozol RNA reagent." BASE COUNT 122 a 257 c 186 g 230 t ORIGIN 1 gcacgaggca aacctttagg ttcctgctct gcaggatggg agactgcact gcaaccggcg 61 cttccggcgc ttatggtgca gtccctggcc ggacctaaat cgttcctttc actcagagcc 121 cgtctccctt attcctatac tttcacttca ttccatctcc ctacgctagc caccaacgtc 181 tgcgcacttt gccgtgttca ctggaacacg atatggtagc aatcgcctcg ttcgattctt 241 ggcctttgag cccctcgttt cttcgcttcg ttacttttgt gccctttgtg tccctttctc 301 ggcactgcct tggaactggt cgcttgtcct cttttgtgga gatctgatgt gatctggggg 361 aaccccggga ggtctggggt tagtgggtgc ttgtggagtc ccccgagacc cacttctggt 421 gccagtgttg tgctttgctt ttccgttttt cctcgtcgcg ctcagcatcg tcaacaagtt 481 cacttcaacc agcagccgtc tccgatggtc tgacgatgac ctttgctgtc ctgcgttcgt 541 ttcccttccg tgaagtggac ttctgccccc cacttcatca ctgctgctac cagcgaagac 601 cttggctcac cccctcggca ctccttggct aaccccctcg gagtttggtc ttcttgcagc 661 cttttcctta gcatgagagg cttctgcact gccgcacctc agcggtggcg agcacggcta 721 ctcgcagaga gaatttgcgc aagcaagacg cttcaacgga gcagcttccc cttccctttc 781 tcctcccata acact //