LOCUS HO046617 398 bp mRNA linear EST 13-SEP-2010 DEFINITION CVSOVPOAN1 Library from post anthesis stages of ovules of polyembryonic Citrus sinensis Citrus sinensis cDNA, mRNA sequence. ACCESSION HO046617 VERSION HO046617.1 DBLINK BioSample: SAMN00169432 KEYWORDS EST. SOURCE Citrus sinensis (sweet orange) ORGANISM Citrus sinensis Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus. REFERENCE 1 (bases 1 to 398) AUTHORS Kumar,V., Pal,D., Malik,S.K., Srinivasan,R. and Bhat,S.R. TITLE cDNA sequences from SSH library of pre and post anthesis stages of ovules of polyembryonic Citrus sinensis JOURNAL Unpublished COMMENT Contact: Shripad Ramchandra Bhat National Research Center on Plant Biotechnology Lab No. 15, Lal Bahadur Shastri Building, Pusa Campus, New Delhi-110 012, India Tel: 91112584178722 Fax: 911125843984 Email: srbhat22@rediffmail.com Seq primer: T7 Promoter. FEATURES Location/Qualifiers source 1..398 /organism="Citrus sinensis" /mol_type="mRNA" /cultivar="Vaniglia Sanguigno" /db_xref="taxon:2711" /clone_lib="SAMN00169432 Library from post anthesis stages of ovules of polyembryonic Citrus sinensis" /dev_stage="Post anthesis" /note="Organ: Ovule; Vector: pGEMT-Easy; SSH libraries prepared from excised ovules collected from pre and post anthesis stages of Citrus sinensis cv. Vaniglia Sanguigno. RNA was isolated from ovules using Trizol method of RNA isolation. SSH was carried both in forward and reverse direction to detect the genes specifically expresses during polyembryonic initials. Clontech PCR-Select cDNA Subtraction kit (Cat. No. 637401) was used to make the libraries. All the sequences were generated as single pass reads, using T7 universal promoter sequence as primer. Sequences submitted are trimmed from low quality sequences and pGEMT-Easy vector backbone. These are the sequences from forward library which includes Tester as post anthesis ovules and Driver as pre anthesis ovules." BASE COUNT 132 a 60 c 90 g 116 t ORIGIN 1 gcgtggtcgc ggccgaggta cggctagcat cttttacaca tttctatgaa gcaaagaatt 61 cgtccaaatc ttcgggagag tttgtaagac tgcgactgat cctgaaagga atgaatggaa 121 aaaacagcat gtcgtatcaa tggataattt tgagaatatt ttattcttgt tcaatcgcta 181 taaaaccaag tttgaattct tggcgcggaa caaaataaat ataattatta agagttgggt 241 cgagttaata aatggataga gccctagggt tccaattata gggaaacaaa aagtaacgag 301 cttcggttct taatttgaat gattatccga tctaattaaa cgttaaaaag atattagttc 361 ctaacgcggg gaaaggtttt cccatgaggg gattatcg //