LOCUS HM475305 2370 bp DNA linear BCT 13-OCT-2010
DEFINITION Bacillus thuringiensis strain IS5059 Vip3A (vip3A) gene, complete
cds.
ACCESSION HM475305
VERSION HM475305.1
KEYWORDS .
SOURCE Bacillus thuringiensis
ORGANISM Bacillus thuringiensis
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
Bacillus cereus group.
REFERENCE 1 (bases 1 to 2370)
AUTHORS Swiecicka,I., Sztachelska,M., Czajkowska,M., Bideshi,D.K. and
Federici,B.A.
TITLE Characterization of Bacillus thuringiensis environmental isolates
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2370)
AUTHORS Swiecicka,I., Sztachelska,M., Czajkowska,M., Bideshi,D.K. and
Federici,B.A.
TITLE Direct Submission
JOURNAL Submitted (07-JUN-2010) Department of Microbiology, University of
Bialystok, 20B Swierkowa Street, Bialystok, Podlasie PL15-950,
Poland
FEATURES Location/Qualifiers
source 1..2370
/organism="Bacillus thuringiensis"
/mol_type="genomic DNA"
/strain="IS5059"
/isolation_source="soil sample from Narew National Park"
/db_xref="taxon:1428"
/country="Poland"
/collection_date="2005"
/PCR_primers="fwd_name: ISvip3A_F, fwd_seq:
atgaacaagaataatactaaattaagcacaagagccttaccaag, rev_name:
ISvip3A_R, rev_seq:
ttacttaatagagacatcgtaaaaatgtacaataggaccaccatataaattattcc"
gene 1..2370
/gene="vip3A"
CDS 1..2370
/gene="vip3A"
/codon_start=1
/transl_table=11
/product="Vip3A"
/protein_id="ADO24139.1"
/translation="MNKNNTKLSTRALPSFIDYFNGIYGFATGIKDIMNMIFKTDTGG
DLTLDEILKNQQLLNDISGKLDGVNGSLNDLIAQGNLNTELSKEILKIANEQNQVLND
VNNKLDAINTMLRVYLPKITSMLSDVMKQNYALSLQIEYLSKQSQEISDKLDIINVNV
LINSTLTEITPAYQRIKYVNEKFEELTFATETSSKVKKDGSPADILDELTELTELAKS
VTKNDVDGFEFYLNTFHDVMVGNNLFGRSALKTASELITKENVKTSGSEVGNVYNFLI
VLTALQAKAFLTLTTCRKLLGLADIDYTSIMNEHLNKEKEEFRVNILPTLSNTFSNPN
YAKVKGSDEDAKMIVEAKPGHALIGFEISNDSITVLKVYEAKLKQNYQVDKDSLSEVI
YGDMDKLLCPDQSEQIYYTNNIVFPNEYVITKIDFTKKMKTLRYEVTANFYDSSTGEI
DLNKKKVESSEAEYRTLSANDDGVYMPLGVISETFLTPINGFGLQADENSRLITLTCK
SYLRELLLATDLSNKETKLTVPPSGFISNIVENGSIEEDNLEPWKANNKNAYVDHTGG
VNGTKALYVHKDGGISQFIGDKLKPKTEYVIQYTVKGKPSIHLKDENTGYIHYEDTNN
NLEDYQTINKRFTTGTDLKGVYLILKSQNGDEAWGDNFIILEISPSEKLLSPELINTN
NWTSTGSTNISGNTLTLYQGGRGILKQNLQLDSFSTYRVYFSVSGDANVRIRNSREVL
FKKRYMSGAKDVSEMFTTKFEKDNFYIELSQGNNLYGGPIVHFYDVSIK"
BASE COUNT 905 a 297 c 434 g 734 t
ORIGIN
1 atgaacaaga ataatactaa attaagcaca agagccttac caagttttat tgattatttt
61 aatggcattt atggatttgc cactggtatc aaagacatta tgaacatgat ttttaaaacg
121 gatacaggtg gtgatctaac cctagacgaa attttaaaga atcagcagtt actaaatgat
181 atttctggta aattggatgg ggtgaatgga agcttaaatg atcttatcgc acagggaaac
241 ttaaatacag aattatctaa ggaaatatta aaaattgcaa atgaacaaaa tcaagtttta
301 aatgatgtta ataacaaact cgatgcgata aatacgatgc ttcgggtata tctacctaaa
361 attacctcta tgttgagtga tgtaatgaaa caaaattatg cgctaagtct gcaaatagaa
421 tacttaagta aacaatcgca agagatttct gataagttgg atattattaa tgtaaatgta
481 cttattaact ctacacttac tgaaattaca cctgcgtatc aaaggattaa atatgtgaac
541 gaaaaatttg aggaattaac ttttgctaca gaaactagtt caaaagtaaa aaaggatggc
601 tctcctgcag atattcttga tgagttaact gagttaactg aactagcgaa aagtgtaaca
661 aaaaatgatg tggatggttt tgaattttac cttaatacat tccacgatgt aatggtagga
721 aataatttat tcgggcgttc agctttaaaa actgcatcgg aattaattac taaagaaaat
781 gtgaaaacaa gtggcagtga ggtcggaaat gtttataact tcttaattgt attaacagct
841 ctgcaagcaa aagcttttct tactttaaca acatgccgaa aattattagg cttagcagat
901 attgattata cttctattat gaatgaacat ttaaataagg aaaaagagga atttagagta
961 aacatcctcc ctacactttc taatactttt tctaatccta attatgcaaa agttaaagga
1021 agtgatgaag atgcaaagat gattgtggaa gctaaaccag gacatgcatt gattgggttt
1081 gaaattagta atgattcaat tacagtatta aaagtatatg aggctaagct aaaacaaaat
1141 tatcaagtcg ataaggattc cttatcggaa gttatttatg gtgatatgga taaattgttg
1201 tgcccagatc aatctgaaca aatctattat acaaataaca tagtatttcc aaatgaatat
1261 gtaattacta aaattgattt cactaaaaaa atgaaaactt taagatatga ggtaacagcg
1321 aatttttatg attcttctac aggagaaatt gacttaaata agaaaaaagt agaatcaagt
1381 gaagcggagt atagaacgtt aagtgctaat gatgatgggg tgtatatgcc gttaggtgtc
1441 atcagtgaaa catttttgac tccaattaat gggtttggcc tccaagctga tgaaaattca
1501 agattaatta ctttaacatg taaatcatat ttaagagaac tactgctagc aacagactta
1561 agcaataaag aaactaaatt gaccgtcccg ccaagtggtt ttattagcaa tattgtagag
1621 aacgggtcca tagaagagga caatttagag ccgtggaaag caaataataa gaatgcgtat
1681 gtagatcata caggcggagt gaatggaact aaagctttat atgttcataa ggacggagga
1741 atttcacaat ttattggaga taagttaaaa ccgaaaactg agtatgtaat ccaatatact
1801 gttaaaggaa aaccttctat tcatttaaaa gatgaaaata ctggatatat tcattatgaa
1861 gatacaaata ataatttaga agattatcaa actattaata aacgttttac tacaggaact
1921 gatttaaagg gagtgtattt aattttaaaa agtcaaaatg gagatgaagc ttggggagat
1981 aactttatta ttttggaaat tagtccttct gaaaagttat taagtccaga attaattaat
2041 acaaataatt ggacgagtac gggatcaact aatattagcg gtaatacact cactctttat
2101 cagggaggac gagggattct aaaacaaaac cttcaattag atagtttttc aacttataga
2161 gtgtattttt ctgtgtccgg agatgctaat gtaaggatta gaaattctag ggaagtgtta
2221 tttaaaaaaa gatatatgag cggtgctaaa gatgtttctg aaatgttcac tacaaaattt
2281 gagaaagata acttttatat agagctttct caagggaata atttatatgg tggtcctatt
2341 gtacattttt acgatgtctc tattaagtaa
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