LOCUS       GT029466                 754 bp    mRNA    linear   EST 02-SEP-2010
DEFINITION  A156 Spider Nephila antipodiana silk glands cDNA library Nephila
            antipodiana cDNA, mRNA sequence.
ACCESSION   GT029466
VERSION     GT029466.1
DBLINK      BioSample: SAMN00168071
KEYWORDS    EST.
SOURCE      Trichonephila antipodiana
  ORGANISM  Trichonephila antipodiana
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Araneae; Araneomorphae; Entelegynae; Araneoidea; Nephilidae;
            Trichonephila.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Huang,W., Lin,Z. and Yang,D.
  TITLE     From EST to potentially novel spider silk gene identification
  JOURNAL   Unpublished
COMMENT     Contact: Yang Daiwen
            Department of Biological Sciences
            National University of Singapore
            14 Science Drive 4, Singapore 117543
            Tel: 65-65161014
            Fax: 65-67792486
            Email: dbsydw@nus.edu.sg.
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Trichonephila antipodiana"
                     /mol_type="mRNA"
                     /strain="Singapore local species"
                     /db_xref="taxon:2730554"
                     /sex="female"
                     /tissue_type="seven types of silk glands, mixed"
                     /clone_lib="SAMN00168071 Spider Nephila antipodiana silk
                     glands cDNA library"
                     /dev_stage="adult"
                     /note="Five adult female spiders of Nephila antipodiana
                     were collected from the bush in east Singapore. The day
                     before the collection, major part of the spider web was
                     destroyed. Tissues comprising all of the seven types of
                     silk glands were isolated and total RNA was extracted from
                     the silk glands using Trizol reagent (Gibco BRL). Poly-A
                     RNA was purified using an Oligo-dT affinity column
                     (Stratagene). For cDNA library construction, Stratagene's
                     Lambda Uni-Zap XR cloning system was used, following the
                     instructions manual from the manufacturer. In brief,
                     5ug of Poly-A RNA was used for cDNA synthesis and two
                     restriction enzymatic digestion sites of XhoI and EcoRI
                     were introduced. cDNA molecules were directionally cloned
                     into the predigested Zap vector and packaged using the
                     package extract. The titer of the primary library reached
                     9x106 and 5x106 primary clones were amplified for long
                     term storage."
BASE COUNT          233 a          165 c          176 g          180 t
ORIGIN      
        1 ctggagctcc cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt
       61 cggcacgagg gcaattgctc gaagagagtg cgctcagttt tatcgttgtt ttttggacat
      121 caattacgtc tgtgagaagc atctacaatg gctctagcag catttttcga tgattacttg
      181 ccattgattc caactcgtcg acgacgaaac cgaccacttg tgctgaatct tttagatttg
      241 ttggaaaatg acatggacca gaaggtcagt caagttgttc gcagattacc tgccactttt
      301 ttggatttcg atgatcacta tgaagaccac agcagaaaac taaagcgcag agcggacgcc
      361 gtgtgttcct atgaacctac acagaaggtc cagatcctca caaacgatga caagttccaa
      421 gtgaaactgg atgcctccaa ctacgaacct ggtgagataa gtgtgaaagt catcaacgac
      481 aggcttgccg tttctgctaa acatgaaact aaagatgacg atgtgtacga ataccacgaa
      541 atggtgagat ccttcgagtt accagaaggt gtagatccag atacagtcgt ttctcgtctg
      601 acttctaatg ggcaattgag catcgaagca cccttaaaac cacaaaaagc agaacaagaa
      661 agagtagttc cagtacagat aacgaaagat tcagaatcga aagataatca gagcataaga
      721 acccgagact cagaaaatga tagtaagtga cttt
//