LOCUS GT029466 754 bp mRNA linear EST 02-SEP-2010 DEFINITION A156 Spider Nephila antipodiana silk glands cDNA library Nephila antipodiana cDNA, mRNA sequence. ACCESSION GT029466 VERSION GT029466.1 DBLINK BioSample: SAMN00168071 KEYWORDS EST. SOURCE Trichonephila antipodiana ORGANISM Trichonephila antipodiana Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae; Araneomorphae; Entelegynae; Araneoidea; Nephilidae; Trichonephila. REFERENCE 1 (bases 1 to 754) AUTHORS Huang,W., Lin,Z. and Yang,D. TITLE From EST to potentially novel spider silk gene identification JOURNAL Unpublished COMMENT Contact: Yang Daiwen Department of Biological Sciences National University of Singapore 14 Science Drive 4, Singapore 117543 Tel: 65-65161014 Fax: 65-67792486 Email: dbsydw@nus.edu.sg. FEATURES Location/Qualifiers source 1..754 /organism="Trichonephila antipodiana" /mol_type="mRNA" /strain="Singapore local species" /db_xref="taxon:2730554" /sex="female" /tissue_type="seven types of silk glands, mixed" /clone_lib="SAMN00168071 Spider Nephila antipodiana silk glands cDNA library" /dev_stage="adult" /note="Five adult female spiders of Nephila antipodiana were collected from the bush in east Singapore. The day before the collection, major part of the spider web was destroyed. Tissues comprising all of the seven types of silk glands were isolated and total RNA was extracted from the silk glands using Trizol reagent (Gibco BRL). Poly-A RNA was purified using an Oligo-dT affinity column (Stratagene). For cDNA library construction, Stratagene's Lambda Uni-Zap XR cloning system was used, following the instructions manual from the manufacturer. In brief, 5ug of Poly-A RNA was used for cDNA synthesis and two restriction enzymatic digestion sites of XhoI and EcoRI were introduced. cDNA molecules were directionally cloned into the predigested Zap vector and packaged using the package extract. The titer of the primary library reached 9x106 and 5x106 primary clones were amplified for long term storage." BASE COUNT 233 a 165 c 176 g 180 t ORIGIN 1 ctggagctcc cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt 61 cggcacgagg gcaattgctc gaagagagtg cgctcagttt tatcgttgtt ttttggacat 121 caattacgtc tgtgagaagc atctacaatg gctctagcag catttttcga tgattacttg 181 ccattgattc caactcgtcg acgacgaaac cgaccacttg tgctgaatct tttagatttg 241 ttggaaaatg acatggacca gaaggtcagt caagttgttc gcagattacc tgccactttt 301 ttggatttcg atgatcacta tgaagaccac agcagaaaac taaagcgcag agcggacgcc 361 gtgtgttcct atgaacctac acagaaggtc cagatcctca caaacgatga caagttccaa 421 gtgaaactgg atgcctccaa ctacgaacct ggtgagataa gtgtgaaagt catcaacgac 481 aggcttgccg tttctgctaa acatgaaact aaagatgacg atgtgtacga ataccacgaa 541 atggtgagat ccttcgagtt accagaaggt gtagatccag atacagtcgt ttctcgtctg 601 acttctaatg ggcaattgag catcgaagca cccttaaaac cacaaaaagc agaacaagaa 661 agagtagttc cagtacagat aacgaaagat tcagaatcga aagataatca gagcataaga 721 acccgagact cagaaaatga tagtaagtga cttt //