LOCUS       GO479265                 613 bp    mRNA    linear   EST 25-NOV-2009
DEFINITION  Aa_aamk2_22C03_T7 Aphelenchus avenae mixed-stage library
            Aphelenchus avenae cDNA clone Aa_aamk2_22C03 5', mRNA sequence.
ACCESSION   GO479265
VERSION     GO479265.1
DBLINK      BioSample: SAMN00167490
KEYWORDS    EST.
SOURCE      Aphelenchus avenae
  ORGANISM  Aphelenchus avenae
            Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
            Tylenchina; Tylenchomorpha; Aphelenchoidea; Aphelenchidae;
            Aphelenchus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Karim,N., Jones,J.T., Okada,H. and Kikuchi,T.
  TITLE     Analysis of expressed sequence tags and identification of genes
            encoding cell-wall-degrading enzymes from the fungivorous nematode
            Aphelenchus avenae
  JOURNAL   BMC Genomics 10 (1), 525 (2009)
   PUBMED   19917084
COMMENT     Contact: Taisei Kikuchi
            Forestry and Forest Products Research Institute
            1, matunosato, Tsukuba, Ibaraki 305-8687, Japan
            Email: kikuchit@affrc.go.jp
            cDNA inserts were sequenced from the 5 end using the T7 primer and
            BigDye 3.1 (ABI) on an ABI 3100 DNA sequencer. Raw sequence trace
            data were processed for submission to dbEST by trace2dbest software
            (http://www.nematodes.org).
            Plate: 22  row: C  column: 03
            Seq primer: T7(GTAATACGACTCACTATAGGGC).
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Aphelenchus avenae"
                     /mol_type="mRNA"
                     /db_xref="taxon:70226"
                     /clone="Aa_aamk2_22C03"
                     /clone_lib="SAMN00167490 Aphelenchus avenae mixed-stage
                     library"
                     /dev_stage="mixed"
                     /note="Vector: PSPORT1; Site_1: SalI; Site_2: NotI; To
                     obtain mixed-stage nematodes, the nematodes were cultured
                     on fungi (Botrytis cinerea grown on autoclaved barley) for
                     3 weeks at 25C and then extracted for 3h at 25C using the
                     Baermann funnel technique. Poly(A)+ RNA was extracted
                     using Sepasol (Nakalai) and subsequently FastTrack MAG
                     micro mRNA Isolation Kits (Invitrogen). The cDNA was
                     synthesized using the SMART technology (Clontech) and
                     cloned into pSPORT1 using an Adaptor (SalI-SmaI, TAKARA).
                     The plasmid construct was transformed into E. coli DH5a by
                     electroporation."
BASE COUNT          146 a          160 c          175 g          132 t
ORIGIN      
        1 tatgggatgc cgcatgtcgc ggacgcgcag tcatccggat ctagtcggaa aagcgtcatc
       61 gtcgtcgtat aacgccgttt cctcttccgc cgacgagaac tcattcgatt ccatccctga
      121 aagtgcgatg ggctccaagt cgtcgaaagc gaacggtcac catcacaatc attccaacgg
      181 ccacgtggtg caggtggcga caatgaacgg cacgcacaag ccggggatcg agaatgcgaa
      241 ttcgccgaat tcacagcggc cgaaagtggg caaggtctcg aatgggcaag cggttttgct
      301 gaggagagac actccagcag gtggactatt cgaaaatccg ccggctgccc cagcgcctcc
      361 tacgtctaac gggtcaagtg acttactgtt ctgtcagaag ccgatctcac aggttgaaag
      421 cgccagtcag gcggacttct tccggatgct ggacgagaaa attgctcaag gtgccaaaga
      481 tattgcggaa tcggatttgg acgactagag cgtcgattcg ttgtgctttc ttgacggagt
      541 atcccaacga aagcgccgtc agagcgggaa gatatcactg ttcgtggtta gctgcttagg
      601 atccttctac ttg
//