LOCUS       GD255082                 151 bp    mRNA    linear   EST 11-MAR-2011
DEFINITION  a0_000303 SARS-Cov infected lung tissue Homo sapiens cDNA clone
            a0_000303 5', mRNA sequence.
ACCESSION   GD255082
VERSION     GD255082.1
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 151)
  AUTHORS   Wu,H., Wang,J., Deng,R., Xing,K., Xiong,Y., Huang,J., He,X. and
            Wang,X. 
  TITLE     Benefits of random-priming: Exhaustive survey of a cDNA library
            from lung tissue of a SARS patient 
  JOURNAL   J. Med. Virol. 83 (4), 574-586 (2011)
   PUBMED   21328370
COMMENT     Contact: Wang XZ
            Department of Biochemistry, School of Life Science, Sun Yat-sen
            (Zhongshan) University
            State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan)
            University
            135# Xingangxi Roud, Guangzhou 510275, People's Republic of China
            Tel: +86-20-84113964
            Fax: +86-20-84113964
            Email: wxz@mail.sysu.edu.cn
            Seq primer: M13 forward
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /db_xref="biosample:166684"
                     /clone="a0_000303"
                     /sex="male"
                     /tissue_type="lung"
                     /dev_stage="autopsy sample, immediately after the death
                     of a SARS patient"
                     /clone_lib="LIBEST_023726 SARS-Cov infected lung tissue"
                     /note="Organ: lung; Vector: pGEM-T-Easy (Promega); The
                     research of the autopsy sample was after informed consent
                     by the patient's family members and in accordance with
                     Helsinki principles. The first-strand cDNA was generated
                     by using random hexamer primers. double-stranded cDNA was
                     ligated into pGEM-T-Easy (Promega) and transformed into
                     E. coli cell DH10B."
BASE COUNT           28 a           46 c           30 g           47 t
ORIGIN      
        1 gcatctcctg cctcagcctc tggtgtagcc aggactacag gcatgcccca ctatgcctgg
       61 cttatttttt ttaattattt tttgtagaca tgagggtctc actgtgttgc ccaggctgct
      121 ctcaaacctc tggcctcaag acatcctccc a
//