LOCUS CP093367 15159 bp DNA circular BCT 19-SEP-2022
DEFINITION Bombilactobacillus folatiphilus strain SG4_D2 plasmid p1unnamed,
complete sequence.
ACCESSION CP093367
VERSION CP093367.1
DBLINK BioProject: PRJNA812348
BioSample: SAMN26370965
KEYWORDS .
SOURCE Bombilactobacillus folatiphilus
ORGANISM Bombilactobacillus folatiphilus
Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
Bombilactobacillus.
REFERENCE 1 (bases 1 to 15159)
AUTHORS Oliphant,S.A., Watson-Haigh,N.S., Sumby,K.M., Gardner,J., Groom,S.
and Jiranek,V.
TITLE Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov.
sp. nov., Bombilactobacillus folatiphilus sp. nov. and
Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid
bacterial isolates from stingless bees Tetragonula carbonaria and
Austroplebeia australis
JOURNAL Int J Syst Evol Microbiol 72 (9) (2022)
PUBMED 36094463
REFERENCE 2 (bases 1 to 15159)
AUTHORS Oliphant,S.A., Sumby,K.M., Gardner,J.M., Watson-Haigh,N.S. and
Jiranek,V.
TITLE Direct Submission
JOURNAL Submitted (11-MAR-2022) Wine Science, The University of Adelaide,
PMB 1, Glen Osmond, South Australia 5064, Australia
COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (PGAP). Information about PGAP can be found here:
https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
##Genome-Assembly-Data-START##
Assembly Date :: NOV-2020
Assembly Method :: Smrtlink v. 9.0
Genome Representation :: Full
Expected Final Version :: Yes
Genome Coverage :: 7404x
Sequencing Technology :: PacBio Sequel II
##Genome-Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Date :: 03/14/2022 11:45:56
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline (PGAP)
Annotation Method :: Best-placed reference protein
set; GeneMarkS-2+
Annotation Software revision :: 6.0
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes (total) :: 1,623
CDSs (total) :: 1,550
Genes (coding) :: 1,527
CDSs (with protein) :: 1,527
Genes (RNA) :: 73
rRNAs :: 4, 4, 4 (5S, 16S, 23S)
complete rRNAs :: 4, 4, 4 (5S, 16S, 23S)
tRNAs :: 58
ncRNAs :: 3
Pseudo Genes (total) :: 23
CDSs (without protein) :: 23
Pseudo Genes (ambiguous residues) :: 0 of 23
Pseudo Genes (frameshifted) :: 12 of 23
Pseudo Genes (incomplete) :: 9 of 23
Pseudo Genes (internal stop) :: 10 of 23
Pseudo Genes (multiple problems) :: 7 of 23
CRISPR Arrays :: 3
##Genome-Annotation-Data-END##
FEATURES Location/Qualifiers
source 1..15159
/organism="Bombilactobacillus folatiphilus"
/mol_type="genomic DNA"
/strain="SG4_D2"
/isolation_source="Bee"
/host="Tetragonula carbonaria"
/type_material="type strain of Bombilactobacillus
folatiphilus"
/db_xref="taxon:2923362"
/plasmid="p1unnamed"
/country="Australia: Brisbane"
/lat_lon="27.4810 S 153.0121 E"
/collection_date="2020-03-20"
gene 52..330
/locus_tag="MOO45_08050"
CDS 52..330
/locus_tag="MOO45_08050"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="UQS82947.1"
/translation="MNGDIIILSRQDHQFRDTINRYNDQIIELIMDILMITNPLIKEK
PKVKAMLVMNLIENSGPAFFKFDSAAQQEAYIDELVMTIYKICFGKES"
gene 533..2329
/locus_tag="MOO45_08055"
CDS 533..2329
/locus_tag="MOO45_08055"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_003712353.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology.
GO_component: GO:0005887 - integral component of plasma
membrane [Evidence IEA];
GO_process: GO:0006855 - xenobiotic transmembrane
transport [Evidence IEA];
GO_process: GO:0009410 - response to xenobiotic stimulus
[Evidence IEA]"
/codon_start=1
/transl_table=11
/product="DHA2 family efflux MFS transporter permease
subunit"
/protein_id="UQS82948.1"
/translation="MSNTKKLISFVGLTIAMFMGTLDSTIINIALPSIMDQFHASLND
TSWVTTIYTLALAVFMITGSKLADRYGRKKLMLIGLVLFGGFSAACMFAPSLLALIVF
RFFQGIGGAIITPIVLPMGIEIFGKENTSKISSVVGAITALAAAGGPPIGGVILQYGS
WRGVFGINLPLSILALLIVVFFIQESYDNTLSGKIDWFGMLALSVSLGGIVFGLLEGR
QLGWHSVIIVTSLVIGVLALLVFYLIEKRVAEPLIELDLIKEKTFTASSLVYFVTGFS
LVCPMLIINYFLQDLLNYSALHAALIIIPVSLTVVVAMPLGTKIFDAMGAKWVTGIGL
LLIAGSLGLLSLIKMNTPTIIIVVFLIINGFGFGFSSVSLVASVQYLPKEKTGIGSGI
VNSMRQIGTCLGMALLVTVLNNNVLNAKNEIRSDAVQTIQTHRLAPQVQNVAEKEVKR
LFKTNSKGKNIKTKTSGLQKKIKRVAQQTNNLPEPAKKSSLGIIYQKQKMMTDGTRTL
NTGLTKLVKDSNNPLLTATGGLVKGQQKMLTGIKLLAQKNEIRTTLRDIKKQKNKKLT
AAFSKTYLLAAIIVLLCSPIALLTDYQKKKVA"
gene 2635..5652
/locus_tag="MOO45_08060"
CDS 2635..5652
/locus_tag="MOO45_08060"
/inference="COORDINATES: protein motif:HMM:NF015347.2"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DUF285 domain-containing protein"
/protein_id="UQS82949.1"
/translation="MFKKVVKVVLLLIFVGSVFLFKRFILSQAANVQPTVMINTKNKD
VVNFLQKLQQLSENTIQDQEATDQPDQHQLISTKVSADIPATGVSGTDGTCAWTLDAA
GNLTINSGTLNQSNDRGIGWDSDHSGFYAYRTNIKQIDFAGPVTAPVNSQYLFQEMSN
LVSFTNLSNFITNQTVDTSYMFYDCRGLIKLDVSNFDTSNVTNMSGMFSSCNNVTHLD
VSNFDTSNVTNMSGMFSSCNNVTHLDVSGFDTSKVTNMSNMFSNCRNTDLDVSNFDTS
NVTSMSGMFSSCNNVTHLDVSGFDTSNVTSMSGMFSSCNNVTHLDVSNFDTSNVTNMS
GMFSSCNNVTHLDVSGFDTSKVTNMSNMFDGCRNTDLDVNGFDTSNVTNMSGMFSSCN
NVTHLDVSGFDTSKVTNMSYMFSICSHLTSLDLSNFDTSKVTNMSYMFQDCSKLPKLD
VGNFDTGKVNYMSGMFSMCYGLTKLDLNNFDTSKVTNMSSMFTACSGLTKLDLSNFDT
SKVTNMSDMFDSCTRLTKLDLNNFDTSKVTNMSDMFDFCPSLWGIQFGSQFIIPSNVS
PSLAMPTIGNTFIDNDKTYLVTAAAWQDVGNGSDHKPTGPVYDNNQSLVSSLSSPHAP
FTYVWQNELQDVKPTLSNVSVTDASTNQTGTTTSALTYCYDSAVAELPSLTVRLGITD
TDSTKFDVYYGIDPVTAITDAKDIVNNANFHKLPASDLNPGSYSITDQTDLCQVAQPK
SGGHRLVFYAVTKDAQGEAVSPGKISQPMTITTKENLYARLHYKNSNGVVIKSAQPVI
FGTAGDVIKTDGSDDLIPPFISHRNTRYQLSTANSFPKIDATTSAGISKNNPKDLDVL
YDVEGAVTVNAPSLDFGKGVPLVKKQYHLQKDSAEKLIVTDTTGAPCWQLSLAITPFS
SDKQNQSIDGIMNYQPVGLIGASPILISQFNETDANNVQTGSECITNISNGWWQDSQQ
TSGPMLDISNQSAEIIPGNYQATATWSVVDSLN"
gene 6101..6373
/locus_tag="MOO45_08065"
CDS 6101..6373
/locus_tag="MOO45_08065"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="UQS82950.1"
/translation="MNQDLAEKADLLVIGLFQDFTENKILELLNFADQKDINIYFLLG
RDMSSLSWLVAKQFLRKKNVQQLPNGLFSHKKMNSYQDQTSWQILH"
gene 6355..7005
/locus_tag="MOO45_08070"
CDS 6355..7005
/locus_tag="MOO45_08070"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="UQS82951.1"
/translation="MADFTLSGLNAALPYNGPFAPSVGHAGQSFFKDASKAIKTSEIQ
CQTFFFLSCNNLIFNDTKLYGPRYNLVLNAIDGYASTIVAAIGVQSADNPELDLVMKH
AGNKNISRMLNKKLSDVQPFVSIIHVGLPENSEVDGTTSAAVRLTPTTKIILSRLSSY
ISSMMLGQEHLIYKLAKNILFDFMQRTRRGTFGVSDAEAKDFELSLINRVNPFSKK"
gene 7014..7205
/locus_tag="MOO45_08075"
CDS 7014..7205
/locus_tag="MOO45_08075"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="UQS82952.1"
/translation="MMNNSVDSLQEFDSYNVYRSVLDKMSIQKMRCECGNVATFCQYL
PEVNGIFPVAIVVVIRQQE"
gene 7211..7543
/locus_tag="MOO45_08080"
CDS 7211..7543
/locus_tag="MOO45_08080"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="UQS82953.1"
/translation="MSNLKFSCDSYNNDDLTIHYRIEITPSEKGDIYYGIQLPSYVEQ
YSTTQFKVTRIKMKTPQTKIITGEIAFSEQTQLQSYYMKLIIVQNGGIQISRSFFNLI
EPNKAKLI"
gene 7617..8156
/locus_tag="MOO45_08085"
CDS 7617..8156
/locus_tag="MOO45_08085"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="UQS82940.1"
/translation="MLSFGRKPLYRPLMVSFVISAVCGLFVGSVFRYDLGFLTFIICF
ILAILNYASKIDILDGFLKIKRPKIYYYDMSTLTKRILLVIFPFRRPQNIDLSSIADY
QLQGDYNKFEDIKLPLTMTTAYGLFFPAILSIKNSMVFNLYLKNSKVIVINLSMDYVY
YPDEFRQKIDSLQSYLPRR"
gene 8294..8839
/locus_tag="MOO45_08090"
CDS 8294..8839
/locus_tag="MOO45_08090"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="UQS82941.1"
/translation="MAKLDPRVRKTKSRIQHELVLMLSYQDINDISITKLCNQAQINR
STFYKHYDSTLEVLEEIESEVIQKFEEYLRQQRRKIDVSNREQMWTMYLQLLQFYQSN
PDMIQALFVYPINKTFKADLLNFSIQYNTYTDSYYQQAYTIAGGLEMIELWVKRDFDL
SPEHLCRLMMEMGPFHDDSSK"
gene 8910..9290
/locus_tag="MOO45_08095"
CDS 8910..9290
/locus_tag="MOO45_08095"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="UQS82942.1"
/translation="MEIGSLAEWITGIAEVAAVVVALFLPSYQARKARKVSQQRIMNL
VRSLLNDLLSKVKATPDMDIAASDEYSDFQKLVSILAVTDADLVNIDILRQIEGLLTD
SDRGDTTEQLVVKVENLMNNYLSK"
gene 9706..10506
/locus_tag="MOO45_08100"
CDS 9706..10506
/locus_tag="MOO45_08100"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_007746911.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="2-keto-4-pentenoate hydratase"
/protein_id="UQS82943.1"
/translation="MTDFNDLTDDQMSLAENLYQAYENHQPLDQAKYAKVLDDEDTAY
QVQQYLMQLKNEPIGGYKVSLTSEETQKMFDSQNPLYGVQVVSHFVPSPSHFRTSQLM
DPLAEVELMFTATQDLQPNDSLEDLMRKTTIAPDIEVPDSRFSDWFPDLSKYLVVADA
AVGGYVVYGRQVPTTQAFASVDAVTQVTCELLHDGQTLKTGAATEVLSNPLLSLQWLV
QKLAAQGVQFLAGQRVSSGTFLLPERVTTGQWQAEFNHDLGDVELTVQ"
gene complement(10617..11255)
/locus_tag="MOO45_08105"
CDS complement(10617..11255)
/locus_tag="MOO45_08105"
/inference="COORDINATES: protein motif:HMM:NF028039.2"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="DUF5067 domain-containing protein"
/protein_id="UQS82944.1"
/translation="MNKGHKLTIITLLSAILLSGCDSKFEIGGMKFDIGGNISTDQNH
PSATTFQNGIFENRQTKIKILKTQVGHSNKTQSDGLIVTFWIQNKSKHRIKPSALLNY
LNFHQSNSSSNYDLRPVFNVAAALFPTINSDGSSTADASTNIGKATDYNQKYVKPLNA
QLLPGKSIKVVRGYELNNKKHPVFATAKKDMHNVDSKSVGTSYKINLQDYKN"
gene complement(11274..12452)
/locus_tag="MOO45_08110"
CDS complement(11274..12452)
/locus_tag="MOO45_08110"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_008856054.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="MFS transporter"
/protein_id="UQS82945.1"
/translation="MKKFYLQSWALILLSFVLGFSEFIIIGILDDLAHQFNVTVATAG
SLVTIFALVYAFSTPVITSLLKNNLYHALLVLIGIFILGNLVTVIAPNYSWLVISRII
VALVSGSLVSLAMTFAAKIAPMEKKAWLVSWIFSGFSIASVFGVPLGTWISTTWGWRI
TFVTIVLFSIIALILIMKVLPHQLNQTQADSVWKQFIIFKDPRIFLSVGIIMFSLAGV
YVFYTYLRPILSQTLGFNHQTITFLLVAYGLMSLLSNQFSGKIANKHGLKIMPWYYAI
EFFVLLFMPKLLTNQLLGVIDILCVGFLMYLINSPIQLHIMGIAEKEYPQSLVLSSSI
NSIFSNLGISLGSAVGGIVTLQFKVQDIGAGGVVFTVVTFVLVLALNHVNRKQSSKVQ
"
gene 12591..13166
/locus_tag="MOO45_08115"
CDS 12591..13166
/locus_tag="MOO45_08115"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="UQS82946.1"
/translation="MVKVDARVCKTKLRIRHELVTMLQHQNINNISITKLCDQAQINR
STFYTHYNSTIEVLEKMESEVLQKSKEYLRQQRQTFDVRDHQKMWVMYLKLLNFYKAN
PDMTRALFVYPINKKFKTDLFNFSDQYNTYTDSYYQQAYTIAGGLEMIELWVKHDFDL
SPERLCKLMVEMGPSLTITVPLIIALMSGQE"
BASE COUNT 4888 a 2315 c 2867 g 5089 t
ORIGIN
1 tttaaagagg ccttaatcaa tggcattgat aaacaatggg aaaataattt tttgaatggt
61 gacatcatca tcttgtcccg tcaggatcat cagtttaggg acactattaa tcgttacaat
121 gaccaaatca ttgaactgat tatggatatt ttgatgatca ctaacccttt aataaaagaa
181 aaacctaaag tcaaagcaat gctggtcatg aatctaattg aaaattcggg ccccgcattc
241 tttaaatttg actctgctgc acaacaagaa gcttatattg atgagttagt tatgacgatc
301 tacaagattt gctttgggaa agagtcataa gcatgaccaa tatgttagaa ggtaaaactt
361 aagtaaaccg tatcaaacag gaaaatgcgc tctaactgaa agtattggta acagtgagct
421 tatatattgg agtattttta tcattgcttg gtgtgctcgg aaaaagagag ttaattagaa
481 aaattaatat taaaaatcat tttaaaaaat tatggattga aggaaagttg agatgtccaa
541 taccaaaaaa ttaattagtt ttgttggttt aacaattgca atgttcatgg gaacgttgga
601 tagtacaatt attaatattg ctttgcctag tattatggat caatttcatg ccagtctgaa
661 tgatacgagt tgggtgacca ccatttatac tttagcgttg gctgtgttta tgatcacagg
721 ttctaaatta gcggatcgtt acggtcgcaa aaagttaatg ctcattggct tggtgctctt
781 tggtggcttt tcagcggctt gtatgtttgc accctcatta ttggctttaa ttgtttttag
841 atttttccaa ggcattggcg gggcgattat cacaccaatt gtgttgccta tggggattga
901 aatttttggg aaagaaaata cgtctaaaat ttcgagtgtc gttggtgcca ttacagcgtt
961 ggcggctgct ggtggaccac cgattggtgg cgttatttta caatatggtt cctggcgcgg
1021 tgtgtttggg attaatttac cattatccat tcttgcccta ttaattgtgg tctttttcat
1081 tcaagaatca tatgacaaca cactatctgg caaaattgat tggtttggta tgctggcact
1141 gtctgtttca ctaggcggaa ttgtcttcgg cttgctggaa ggtcgacaat taggttggca
1201 ttcagtgatt attgtgacta gtttagtaat tggcgtttta gcgctattgg tcttttacct
1261 aattgaaaaa cgcgttgcgg aaccgttaat tgaactagat ctaatcaagg aaaagacttt
1321 tacagcatcc agcttagttt attttgtgac aggcttttca ttagtctgcc ctatgttaat
1381 tattaattat tttttacaag atttactcaa ttacagcgca ctgcatgccg cgttgattat
1441 tattccggta tcgttgacgg tggttgtagc gatgcctttg ggaacgaaaa tttttgatgc
1501 aatgggtgcc aaatgggtca caggaatcgg actattattg attgcgggta gtttaggctt
1561 gctatcatta atcaaaatga ataccccaac cattattatt gttgtttttc tgattattaa
1621 tggttttggc tttggttttt catcggtttc acttgttgct tcggtccaat atttgcctaa
1681 agaaaaaacg gggattggtt cgggaatcgt taattcgatg cgtcaaattg gtacgtgtct
1741 aggcatggcg ttattggtga cagttttaaa caacaatgtt ttaaatgcga aaaatgaaat
1801 tagaagtgat gcagttcaaa cgattcaaac ccatcgtttg gcgccacagg ttcagaatgt
1861 ggcagaaaaa gaagttaaac gtctttttaa aacgaattcc aagggtaaaa atattaaaac
1921 gaaaacttct ggtttacaga aaaaaatcaa acgggtagct cagcaaacta ataatttacc
1981 tgaacctgct aaaaagagtt ctttaggaat catttatcaa aaacaaaaaa tgatgactga
2041 tggcaccaga acgctcaata ctggtctcac taaattggtg aaagatagca ataatccttt
2101 gttgacggcg actggtggtt tagtgaaggg acaacagaag atgttgaccg gtattaagtt
2161 gttggcacaa aagaacgaaa tcagaaccac gttaagggac attaagaaac aaaaaaataa
2221 aaaactaacg gctgcattta gtaaaactta tttacttgcc gcgatcattg tcttgctgtg
2281 ttcgccaatt gcgttgttaa cggattatca aaagaaaaaa gtagcttaag tatttaaatt
2341 aacacttccc agagagctga tgaaaatcgg ctcttttttt ggttacaatt gttccacatc
2401 atacttatac ggagattgtt attgcttata tataatctgg aaattaatta aattgaagat
2461 aaattattaa gttaattgca aactttcagc tgagggttta agatgaagat gatttcgcaa
2521 acatattatt agctaaaagg ataattaata tgttaaaatg ataagttttg tttattgaaa
2581 atctgtaatc gtgatggcgt ctttggttaa cattagtgag aggacaagag cctgatgttt
2641 aaaaaagttg tgaaagtcgt tttactttta atttttgtcg gtagcgtttt cttatttaaa
2701 agattcattt tatcccaagc tgctaatgtt caaccaactg tcatgattaa taccaaaaat
2761 aaagatgtgg ttaatttttt acaaaaatta caacaattat ctgagaatac aatccaagat
2821 caggaagcaa cagatcagcc tgaccagcac caattgatta gtacgaaagt gtctgccgac
2881 attccagcta cgggtgtgag cggaactgat ggaacttgtg catggacttt agatgcagct
2941 ggtaatttaa cgattaatag cggcacttta aatcagagta atgatagagg tattggttgg
3001 gacagtgatc attctggctt ttatgcttat agaacgaata ttaaacaaat tgattttgcg
3061 ggtccagtca ctgcaccagt taatagtcaa tatttgtttc aggaaatgtc aaaccttgta
3121 tcatttacca atctgtctaa tttcataaca aatcagactg ttgatacgtc ttatatgttt
3181 tatgactgtc gggggctaat caaattggat gtaagtaatt ttgatactag taatgtaact
3241 aatatgtctg gtatgtttag tagttgcaac aacgtaactc atttggatgt aagtaatttt
3301 gatactagta atgtaactaa tatgtctggt atgtttagta gttgcaacaa cgtaactcat
3361 ttggatgtaa gtggttttga taccagtaaa gttacgaata tgtctaatat gtttagtaat
3421 tgccgcaata ctgatctgga tgtaagtaat tttgatacta gtaatgtaac tagtatgtct
3481 ggtatgttta gtagttgcaa caacgtaact catttggatg taagtggttt tgatactagt
3541 aatgtaacta gtatgtctgg tatgtttagt agttgcaaca acgtaactca tttggatgta
3601 agtaattttg atactagtaa tgtaactaat atgtctggta tgtttagtag ttgcaacaac
3661 gtaactcatt tggatgtaag tggttttgat accagtaaag ttacgaatat gtctaatatg
3721 tttgatggtt gccgcaatac tgatctggat gtaaatggtt ttgatactag taatgtaact
3781 aatatgtctg gtatgtttag tagttgcaac aacgtaactc atttggatgt aagtggtttt
3841 gataccagta aagtgactaa tatgtcttat atgtttagta tttgtagtca cttaactagt
3901 ttagatttga gtaattttga tactagtaaa gtgactaata tgtcttatat gtttcaggat
3961 tgtagcaaat tgcccaagtt agacgtcggc aattttgata ctggtaaagt gaactatatg
4021 tctggtatgt tttctatgtg ttatggttta actaaattgg atttgaataa ttttgatact
4081 agtaaagtga ctaatatgtc tagtatgttt acagcttgca gtggtttaac taaattggat
4141 ttgagtaatt ttgataccag taaagttacg aatatgtctg atatgtttga tagttgcact
4201 agattaacta aattggattt gaataatttc gatactagta aagtgactaa tatgtctgat
4261 atgtttgatt tttgtcctag tctttggggg attcaatttg gttcgcaatt tataattcca
4321 tccaatgtat caccttcttt agctatgcct acaataggta atacttttat tgataatgac
4381 aaaacctatc ttgtcactgc tgctgcatgg caagatgtcg gcaatggcag tgaccataag
4441 cctacgggtc ctgtctatga taataatcaa tcacttgtat catctttaag ttcgccacac
4501 gcaccgttta cttatgtttg gcaaaatgag ttgcaggatg tgaagccaac attatccaat
4561 gtatcggtta cagatgcaag tacgaatcaa actgggacaa caacttccgc attaacttat
4621 tgttatgatt cagcggttgc tgagttgcct agtttaactg tgagactcgg gattaccgat
4681 accgatagca ccaaatttga tgtttactat ggtattgatc ctgtaaccgc tattacggat
4741 gctaaagata tcgtgaataa tgccaatttt cataagcttc ctgcgtctga tttgaaccct
4801 ggtagttatt cgatcaccga tcaaactgat ttatgccaag tagctcaacc taagtctggt
4861 ggccatcggt tagtctttta tgctgtgacc aaagatgccc aaggcgaagc tgttagcccg
4921 gggaagattt cacagccgat gacgattacg accaaagaga atctttacgc tagattgcat
4981 tataagaatt caaacggtgt tgtcattaaa agtgctcaac ctgttatttt cggaactgcg
5041 ggtgatgtta tcaaaactga cggttctgac gatttaatcc ctccttttat tagtcatagg
5101 aacactcgtt atcaactcag cactgctaat tcattcccta aaattgatgc tacgacctca
5161 gctggcattt ctaaaaataa tcctaaagat ttagatgtcc tttatgacgt tgagggtgcg
5221 gtcacagtta atgcgccgag tcttgatttt ggtaaggggg tgcctttggt taaaaagcaa
5281 tatcacttgc agaaagattc cgccgaaaag ttaattgtga cagataccac gggagctcct
5341 tgttggcagc tatcgttagc gatcacacct ttttcaagtg ataagcaaaa tcaaagtatt
5401 gatggaatta tgaattatca gcctgtgggc ttaattggcg ccagtccgat tctgattagt
5461 caatttaatg aaacggatgc taataatgta cagacgggtt ctgaatgtat taccaatatt
5521 tcgaatggtt ggtggcaaga tagtcagcaa acgagtggtc cgatgctaga tatttcgaat
5581 caatctgccg agattatacc tggtaactat caagctacgg cgacttggtc ggtggtcgat
5641 tcattaaatt gatggaagca tcacgcatta tcatcgtgtt gttcggatta attatagaat
5701 ctgtggaaat cataagcctt ttgagcaggc taaatctggt ctgcttaaaa ggcttattta
5761 gtacggcttt ttagttaata aaattatttt ttaatttatt ttaacactaa ctttaataaa
5821 cgaatgattt aattctttta aaaggagaat acgattttaa tgatctggat agatattttt
5881 atgaaataaa ccaaatagat agattcgaaa atgaaatgcc agtttatata aatgataaac
5941 gtagggattt gttagaaaag atagctctaa gtaatacaac agatttagaa tttgtgcgcc
6001 ctatcaaagc tgatttagga gtaatccttt ctaaggaagc gctattatat gaaacaaatc
6061 agttgcttgc taacaagtgg actactgatc cagaaaccgt atgaaccaag atttggctga
6121 gaaagctgat ctgttagtta tcggtttgtt ccaagatttt actgagaata aaattttaga
6181 gctgcttaat tttgctgacc agaaagatat aaatatttac tttttgttag gtcgagatat
6241 gtcgtctttg tcttggctag tagccaaaca gtttttgaga aagaaaaatg ttcagcaatt
6301 gcctaatgga ttgttttcac ataaaaaaat gaattcttat caggatcaaa catcttggca
6361 gattttacac taagcggctt aaatgcagcg ctaccatata atggtccttt tgctccttca
6421 gttggtcatg caggacaatc cttttttaaa gatgccagta aagccatcaa aacctcagag
6481 attcaatgtc aaaccttttt ctttttatcc tgcaataatt taatttttaa tgatacgaaa
6541 ttatatggac caagatacaa tttagtactc aatgctattg atggttatgc ttcgaccata
6601 gttgccgcga taggtgttca atcagcagat aatccggaat tagatttagt catgaagcac
6661 gcgggaaata aaaatatatc acggatgtta aataaaaaat tgtccgatgt gcaacctttt
6721 gtatctatta ttcatgtggg actacctgaa aatagtgaag ttgatggaac aacatccgca
6781 gctgttaggt taactccaac cacaaaaatt attttatcta gattgtcgtc atatatttct
6841 tccatgatgc taggacaaga gcatttaatt tataaattag caaaaaacat tttgtttgac
6901 tttatgcaac gtactcggcg tggaactttt ggtgtttctg atgctgaagc taaagatttt
6961 gaattgtcgt tgatcaatcg tgtgaaccct ttttctaaaa aatagctgaa ttaatgatga
7021 ataattcagt agattcactg caagaattcg atagttataa tgtttatcgt tcggttctag
7081 ataagatgag tattcaaaaa atgcgctgtg aatgtgggaa tgtagcaact ttttgtcagt
7141 atttgccaga agttaatggg atatttccgg ttgctatcgt tgtggtgata agacaacagg
7201 aatgaaaaac atgtctaatt taaaatttag ttgtgattcc tataacaatg atgacttaac
7261 gatccattat aggattgaaa ttacccccag tgagaagggt gatatttatt atgggataca
7321 actgcctagt tatgtagaac aatattctac aacacagttt aaagtgactc gaatcaaaat
7381 gaaaacaccg cagactaaaa ttattactgg agagattgcc ttttctgagc aaacccagtt
7441 acaaagttat tatatgaaac taatcatcgt tcaaaatgga ggaatccaaa tcagtagaag
7501 cttttttaat ttgattgaac caaacaaagc taaactaatt tgatgtacaa tttattttgg
7561 acgttatggg taatgaataa tattgttaat atttgtgttg ggagtggtga tacattatgt
7621 tatcttttgg tagaaaacca ctatatcgac cgttaatggt tagttttgtt attagtgcag
7681 tttgtggttt gtttgttggt tccgtgttta gatacgattt gggcttttta acttttatca
7741 tttgctttat tttggctatt ttaaattatg ccagcaagat tgatatttta gatggttttt
7801 taaaaattaa gcgcccaaaa atttattact atgatatgag tactttgacg aaacgtattc
7861 tgttagtgat atttccgttt aggcggccgc aaaatatcga tctaagttct attgcagatt
7921 atcaattgca aggtgactat aataagtttg aggatatcaa attacccttg accatgacta
7981 ctgcttatgg cctctttttt ccggctatct tatcaattaa aaattctatg gttttcaatt
8041 tgtatcttaa gaattcaaaa gtgattgtta ttaatttatc aatggactat gtttactatc
8101 ctgatgaatt tagacaaaag atagattcac tacaatctta tttgccacgc agatagagtg
8161 gtgaaaaatt gatgacaaaa aaagttactt attaataata aattattccg aattcaattc
8221 agtattttat aaaaaaatgc tgtatcgtaa aagtatgtat tcaatatttt tttgttaagg
8281 agatagtgtt tcgatggcca agttagatcc ccgagtccgg aaaacgaaat caagaattca
8341 acacgagtta gtattgatgc tcagttatca agatattaat gatatttcta ttactaaatt
8401 gtgtaatcaa gcgcaaatta accgttcgac tttttacaag cattacgatt caacccttga
8461 agtcttggaa gaaattgaat ctgaagtgat tcaaaaattt gaagaatatt tacgtcaaca
8521 acgtcggaag attgatgtgt ccaatcgtga acaaatgtgg acgatgtatt tacagctgtt
8581 gcagttttat caatccaatc cagacatgat tcaagctttg tttgtgtatc cgatcaataa
8641 aactttcaaa gcagatttgc tcaatttttc gattcaatac aatacttata cggatagtta
8701 ttatcaacaa gcttatacaa ttgcgggtgg cttagagatg attgaattgt gggtcaagcg
8761 tgactttgat ttgtcaccgg agcatctttg tcggttaatg atggagatgg gaccatttca
8821 tgatgattct tccaagtaaa gtgcattctc aattattgtc tgctataatt ttcatttaaa
8881 ttataaattt ggtaaagggg tctagtctta tggaaatagg cagtttagcc gagtggatta
8941 ctggcattgc agaggtggcg gccgtggttg tagctctatt tttaccatct tatcaggctc
9001 gcaaagcgcg caaagttagt cagcagcgga taatgaattt agtgcgcagc ttgttgaatg
9061 acttattgtc taaagttaaa gcaactccgg atatggatat tgcagcgtct gatgaatata
9121 gtgattttca aaagctagtt tccatcttgg cagttaccga tgctgattta gtcaatattg
9181 atattttgcg acaaattgag ggcttattaa cagatagcga tcgtggagat acaaccgaac
9241 aacttgtcgt taaagtagaa aacctgatga ataattatct gtcaaaatag tgattcacat
9301 cgtgttgact gatcttttct tgacaaacac caaataatat tgtaaattgc tcataacaat
9361 taaaataaaa ttaaggctca tgaaggaaac tttatcttgt gggtacagtc agcgagttgt
9421 cggttggtgc aagacaatct gtcaaacaag attatgatca gcctaaaaga gtgcagtgct
9481 gaaatttagt aagcactgct ggtggacaat cattaacatg gatgcaaaat gaatacgttt
9541 tgtagagtgg tatttaagct gtttaaataa ctaaaataag gtggtaccgc gttaattgac
9601 gcccttagca aaaaatgcta agggcgtttt tttaatttaa ggaggtgagg caaaatttca
9661 gatcttgttg ggattagttt aggagccaat ttaggaggaa aaaggatgac cgattttaac
9721 gatttaacag acgaccaaat gagtttagca gagaatttat atcaagctta tgaaaatcat
9781 cagccgttag accaagctaa atatgcaaaa gttttggatg atgaggatac ggcttaccag
9841 gttcaacaat atttaatgca actaaaaaat gaaccgattg gtggctacaa ggtgtctttg
9901 acgagtgagg aaactcaaaa aatgtttgat tctcaaaatc cgctatatgg tgtgcaagta
9961 gttagtcatt tcgtaccatc accttcgcat tttcgaactt cacaactgat ggatccacta
10021 gccgaggtgg aattgatgtt tacagccacg caagatttac aaccaaatga tagcttagaa
10081 gatttaatgc gaaaaacgac gatagccccc gacattgagg ttcctgattc ccgtttttcg
10141 gattggtttc ccgatttgtc taagtactta gtggttgctg acgcagctgt gggcgggtac
10201 gttgtttatg gccgacaagt tcccaccact caagcttttg cctcagttga tgccgttacg
10261 caagtaactt gtgaattatt gcatgacggt caaacactca agacaggtgc tgcaacagaa
10321 gttttgagta acccattgtt gtctttacaa tggttggtac agaaactggc ggctcaaggt
10381 gttcaatttt tagcaggaca gcgtgtttcc agcggaactt ttttgttgcc ggaacgtgtc
10441 actacaggtc aatggcaggc cgaatttaat catgatttag gtgatgtaga gttgacagtg
10501 cagtaaatgc tagatactga tttgtcctgc gtttatgtct aaaggtttct acagtttaaa
10561 tcaaaaaaat attaaaggac taacttcgaa atttaatcga ttttagtcct tttttgttaa
10621 tttttataat cctgcaagtt gattttataa gaggttccta cagacttact atccacgtta
10681 tgcatatctt ttttggcagt agcaaaaacc ggatgcttct tattatttaa ttcatagccc
10741 cggactactt tgatgctttt accaggtaat aattgagcgt ttaaaggttt aacgtatttt
10801 tggttatagt cagtagcttt accaatattt gtactggcat ctgctgttga agatccatct
10861 gaattaatag tgggaaagag tgccgcggca acattaaaaa ctggccttaa gtcataattt
10921 gaactgctat tgctttggtg gaagttcaga tagtttaata aagctgacgg tttaattcga
10981 tgcttcgatt tgttttggat ccagaaagta acgattaaac cgtcgctttg agttttgttt
11041 gaatggccaa cttgggtttt taaaattttg attttagttt gcctattttc aaaaattcca
11101 ttctgaaaag tggttgcgga cggatggttt tgatcagtag atatattgcc gccaatgtca
11161 aatttcatac caccaatctc aaactttgaa tcacatcctg ataataatat ggctgataat
11221 aaagtaatga tggttaattt gtgaccctta ttcatatata tctttctcct taattattga
11281 acttttgagg attgtttcct gttcacgtga tttaacgcta agactaagac gaaggttacg
11341 actgtaaaaa ccactccgcc tgccccaata tcttggactt tgaattgtag tgtgacaatg
11401 ccccctacgg ctgaacccaa cgaaatacct aaatttgaaa aaatcgaatt aattgatgat
11461 gataaaacga ggctttgggg atattctttt tctgcaatgc ccattatgtg taattgaata
11521 ggcgaattaa ttaagtacat caaaaagcca acacataaaa tatcaatgac ccccagcaat
11581 tgattagtta ataacttcgg catgaatagt aaaacgaaaa attcgatagc gtaataccac
11641 ggcattattt ttaaaccgtg tttatttgca attttgccac tgaattgatt acttaataat
11701 gacatcaaac cataggcaac taataagaag gtgatcgttt gatgattaaa gcctagagtt
11761 tgggacaaga ttggccgtaa ataagtataa aaaacatata cccctgctaa gctaaacata
11821 ataattccaa cacttaaaaa aattcgagga tctttgaaaa ttataaattg tttccaaacg
11881 gaatctgctt gtgtttgatt taattgatga ggtaatactt tcataattaa aattagggcg
11941 ataatgctaa ataaaacaat cgtcacaaaa gttatgcgcc atccccaagt tgtactaatc
12001 caggtaccta gtggtacacc gaagactgat gcaatactga atccagaaaa aatccagctc
12061 acgagccaag cttttttctc catcggggca attttggctg cgaaagtcat ggctaaagaa
12121 actaaagaac cagaaactag tgctacaatg atacgagaaa taactaacca actataatta
12181 ggtgcgataa cggttactaa gttgcctaag ataaagatgc caattaaaac tagtaatgcg
12241 tggtagagat tgttctttaa taatgaagtt ataacgggcg tactaaaagc ataaaccaac
12301 gcaaagattg ttactaacga ccctgcagtt gccacggtaa cattgaattg atgtgctaaa
12361 tcatccaaaa tgccaataat gatgaattca ctgaacccta aaacaaatga taataaaatc
12421 aaagcccagc tttgtaagta gaattttttc atgctattcc tcgtcttttt actaatatta
12481 ggttataaaa aattaaaggt agttgtcaaa tacatttact aattatttga taaaatgttt
12541 aatatttatg ttcaaattat tgtattggtc agtaaggaga tacttctaca atggtaaaag
12601 ttgatgcgag agtatgtaaa actaaattaa gaattcgaca tgaattggta acgatgctac
12661 agcatcaaaa tattaataat atttctatta cgaaattatg tgatcaagcg cagattaatc
12721 gttccacttt ttatacacat tacaactcaa cgattgaggt tttggagaaa atggaatcag
12781 aagtccttca aaaatccaag gaatatttac gccagcagcg tcaaacgttt gatgtacgtg
12841 atcatcaaaa aatgtgggtc atgtatttga aacttttaaa tttttataag gctaatcctg
12901 atatgactcg ggctttgttt gtttatccta ttaataaaaa attcaagaca gatttgttta
12961 atttttcaga tcaatataac acttatacag atagttatta tcaacaggct tatacgattg
13021 caggtggctt agagatgatt gaattgtggg ttaagcatga ctttgatttg tctcctgagc
13081 gcttgtgcaa attgatggtg gagatgggcc cctctttaac gataacggta ccacttataa
13141 tcgctttaat gagcggtcaa gaataagggc ctgatctatc aagcgcccgt ttttattagc
13201 tatacggatt cgcatgatac ttttttcgat caagcattga ccaagttcaa taaaaataat
13261 aaatgtagaa tcagatgaat tggctaagtt taagcttttt gtgtttatct ggcttaaaaa
13321 agtataattt atcaacaaat attaaaacta tatagataga ttttataaat tactatgggc
13381 ttaagaacta agttaaaaaa tacgatttaa tgatagttgc tgatggtaat gtttacttta
13441 aggaaatcaa agtaatactt agaagtgaag aatcttatcg attgttataa aacagatttt
13501 aaacaaaaaa tatgtttgaa gtattgtcaa catatatcga tacgtgttgg caattaatta
13561 tgtcatgagg caagagatca catatcaaaa ttcaaaaatt agataaaaga ccggttatta
13621 ttctgcatgg cgaactatct tctaaagaga agctggatta aagtaaacgg cttttttgtg
13681 acttttaaag gggatatttt catgttagtc tttatcattt tccgcgattt tgataatagt
13741 gtagctcaag gagttaatga aaatgatttt atacggatgg cttcagatta ttttaaatcg
13801 aatctgacgg ataattatgt tagtcgattg ataaatttct aaactgttaa atttagtgtc
13861 tattttgtta agtaaaaaca atctatatga gaataaactt gattataata aaggtttagt
13921 atagtgattt agctttccga aagtattcgc tgattctttt ttagtgtaga ttataaaaaa
13981 agttaataaa actatagtct tattgctaaa aaagcggtga atcttttatt ttatctggaa
14041 tagttagaga tataggacaa taaaaaaaga cgtgtcaaaa taacacagta tatatatata
14101 tatactgtgt gtcataataa tacgaaaaat aagagtccta tgagctatag ctcataggac
14161 tcttattttt cgtattatta tgacacacag tatatatata tatatactgt gttattttga
14221 cacgtctttt tttattgtcc tatatctcta actattccag ataaaataaa agattcaccg
14281 cttttttagc aataagacta tagttttatt aacttttttt ataatctaca ctaaaaaaga
14341 atcagcgaat actttcggaa agctaaatca ctatactaaa cctttattat aatcaagttt
14401 attctcatat agattgtttt tacttaacaa aatagacact aaatttaaca gtttagaaat
14461 ttatcaatcg actaacataa ttatccgtca gattcgattt aaaataatct gaagccatcc
14521 gtataaaatc attttcatta actccttgag ctacactatt atcaaaatcg cggaaaatga
14581 taaagactaa catgaaaata tcccctttaa aagtcacaaa aaagccgttt actttaatcc
14641 agcttctctt tagaagatag ttcgccatgc agaataataa ccggtctttt atctaatttt
14701 tgaattttga tatgtgatct cttgcctcat gacataatta attgccaaca cgtatcgata
14761 tatgttgaca atacttcaaa catatttttt gtttaaaatc tgttttataa caatcgataa
14821 gattcttcac ttctaagtat tactttgatt tccttaaagt aaacattacc atcagcaact
14881 atcattaaat cgtatttttt aacttagttc ttaagcccat agtaatttat aaaatctatc
14941 tatatagttt taatatttgt tgataaatta tactttttta agccagataa acacaaaaag
15001 cttaaactta gccaattcat ctgattctac atttattatt tttattgaac ttggtcaatg
15061 cttgatcgaa aaaagtatca tgcgaatccg tatagctaat aaaaacgggc gcttgataga
15121 tcaggccctt attcttgacc gctcattaaa gcgattata
//