LOCUS CK909750 299 bp mRNA linear EST 11-MAR-2004 DEFINITION hasp018xa01 Heterobasidion annosum - Scots pine infection stage subtraction cDNA library (hasp) Pinus sylvestris/Heterobasidion annosum mixed EST library cDNA clone hasp018xa01, mRNA sequence. ACCESSION CK909750 VERSION CK909750.1 DBLINK BioSample: SAMN00166031 KEYWORDS EST. SOURCE Pinus sylvestris/Heterobasidion annosum mixed EST library ORGANISM Pinus sylvestris/Heterobasidion annosum mixed EST library Eukaryota; mixed EST libraries. REFERENCE 1 (bases 1 to 299) AUTHORS Asiegbu,F.O. and Li,G. TITLE Expressed sequence tags of randomly selected cDNA clones from the interaction of the root rot fungus (Heterobasidion annosum, P-type) with seedling roots of Scots pine (Pinus sylvestris) JOURNAL Unpublished COMMENT Contact: Fred O. Asiegbu Dept. of Forest Mycology & Pathology Swedish University of Agriculture, Box 7026,S-750 07, Uppsala, Sweden Tel: +46 18 67 15 98 Fax: +46 18 30 92 45 Email: Fred.Asiegbu@mykopat.slu.se Seq primer: T7 primer. FEATURES Location/Qualifiers source 1..299 /organism="Pinus sylvestris/Heterobasidion annosum mixed EST library" /mol_type="mRNA" /db_xref="taxon:169015" /clone="hasp018xa01" /clone_lib="SAMN00166031 Heterobasidion annosum - Scots pine infection stage subtraction cDNA library (hasp)" /dev_stage="Seedling roots of scots pine were infected for 6 days with H. annosum" /note="Vector: pT-Adv; Site_1: EcoRI; The subtractive hybridization cDNA library was constructed from scots pine roots infected for 6-days with mycelia of Heterobasidon annosum (FP5)." BASE COUNT 68 a 70 c 88 g 73 t ORIGIN 1 tcgagcggcc gcccgggcag gtacgcgggg gaagcaggct gctgcgttat ctgataactt 61 cataacttcc cagtggagta gtttgttctt ctcttttctt catttttgtt gccaatatgt 121 cgagcgattg cgcaaattgt gactgcgctg acaaaaccca gtgccccaag aagaagggat 181 tccagatgga tggtattgtg gagaccagct atgagatggg gcatggtgga aatgcatttc 241 tggaaaatga ctgcaagtgc ggccccaact gcaagtgcgg tacctcggcc gcgaccacg //