LOCUS CD268968 291 bp mRNA linear EST 03-FEB-2011 DEFINITION T143A04594F (FHIG:A) Ectomycorrhiza plate culture Betula pendula/Paxillus involutus mixed EST library cDNA 5', mRNA sequence. ACCESSION CD268968 VERSION CD268968.1 DBLINK BioSample: SAMN00173256 KEYWORDS EST. SOURCE Betula pendula/Paxillus involutus mixed EST library ORGANISM Betula pendula/Paxillus involutus mixed EST library Eukaryota; mixed EST libraries. REFERENCE 1 (bases 1 to 291) AUTHORS Johansson,T., Le Quere,A., Ahren,D., Soderstrom,B., Erlandsson,R., Lundeberg,J., Uhlen,M. and Tunlid,A. TITLE Transcriptional responses of Paxillus involutus and Betula pendula during formation of ectomycorrhizal root tissue JOURNAL Mol. Plant Microbe Interact. 17 (2), 202-215 (2004) PUBMED 14964534 COMMENT Contact: Johansson,T. Fungal-Host Interaction Group (FHIG) Microbial Ecology, Institution of Ecology Ecology Building, Lund University, SE-223 62 Lund, Sweden Tel: +46 46 222 45 49 Fax: +46 46 222 41 58 Email: tomas.johansson@mbioekol.lu.se PCR PRimers FORWARD: P104 (5'-GGGAAGCGCGCCATTGTGTT-3') BACKWARD: P105 (5'-AGTGAGCTCGAATTGCGGCC-3') Seq primer: P104. FEATURES Location/Qualifiers source 1..291 /organism="Betula pendula/Paxillus involutus mixed EST library" /mol_type="mRNA" /strain="ATCC200175(P.involutus); Skuleskogen,Skogsforsk,Sweden(B.pendula)" /db_xref="taxon:231415" /tissue_type="Ectomycorrhizal root tissue" /clone_lib="SAMN00173256 (FHIG:A) Ectomycorrhiza plate culture" /dev_stage="25 days of growth after transfer/synthesis" /lab_host="Escherichia coli BM25.8" /note="Vector: pTriplEx2; Site_1: SfiI; Site_2: SfiI; This EST clone is originating from one of three cDNA libraries, constructed for transcript profiling of the mycorrhizal interaction between the basidiomycete Paxillus involutus and Betula pendula (birch). One library represents the developed and functional mycorrhizal root tissue ('(FHIG:A) Ectomycorrhiza plate culture'), a second library represents axenically grown fungus ('(FHIG:B) Axenic plate culture') and a third library represents axenically grown plants ('(FHIG:C) Axenic plate culture'). Libraries were analyzed in parallel and 3555 (FHIG:A), 3964 (FHIG:B), and 2532 (FHIG:C) high-quality (PHRED 20) ESTs of >99bp have been deposited. The cDNA libraries were constructed from total RNA using the SMART cDNA library Construction kit (#K1051-1, Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Full-length cDNAs were trimmed by SfiI, fractionated and directionally ligated into (lambda)TriplEx2 arms. The lambda library was converted to a plasmid library via site-specific recombination at loxP sites in a Cre+ strain (E. coli BM25.8). Plasmid clones were randomly collected and analysed by DNA sequencing using a plasmid-specific forward primer (P104)." BASE COUNT 78 a 58 c 79 g 76 t ORIGIN 1 ctttttggac tgcccatcag cttcagtgtt gttcgtaacg ctttcttcgg tggttgggcc 61 gagaccattg agctcgacat tcctcctcat cgcagcagta gcggataggg agagatcgtt 121 ggcaatgacg aatcttgagg attgtcagaa atggaaaggg tggtgtactt cgggaacaaa 181 cctcacaaga ggtattccct tggcataccg gatggagtcg taactccagt tgctgaaagt 241 gcctcgagaa ggacgaattt atatggttga tactttggaa aaaaaaacaa a //