LOCUS       CD268968                 291 bp    mRNA    linear   EST 03-FEB-2011
DEFINITION  T143A04594F (FHIG:A) Ectomycorrhiza plate culture Betula
            pendula/Paxillus involutus mixed EST library cDNA 5', mRNA
            sequence.
ACCESSION   CD268968
VERSION     CD268968.1
DBLINK      BioSample: SAMN00173256
KEYWORDS    EST.
SOURCE      Betula pendula/Paxillus involutus mixed EST library
  ORGANISM  Betula pendula/Paxillus involutus mixed EST library
            Eukaryota; mixed EST libraries.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Johansson,T., Le Quere,A., Ahren,D., Soderstrom,B., Erlandsson,R.,
            Lundeberg,J., Uhlen,M. and Tunlid,A.
  TITLE     Transcriptional responses of Paxillus involutus and Betula pendula
            during formation of ectomycorrhizal root tissue
  JOURNAL   Mol. Plant Microbe Interact. 17 (2), 202-215 (2004)
   PUBMED   14964534
COMMENT     Contact: Johansson,T.
            Fungal-Host Interaction Group (FHIG)
            Microbial Ecology, Institution of Ecology
            Ecology Building, Lund University, SE-223 62 Lund, Sweden
            Tel: +46 46 222 45 49
            Fax: +46 46 222 41 58
            Email: tomas.johansson@mbioekol.lu.se
            PCR PRimers
            FORWARD: P104 (5'-GGGAAGCGCGCCATTGTGTT-3')
            BACKWARD: P105 (5'-AGTGAGCTCGAATTGCGGCC-3')
            Seq primer: P104.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Betula pendula/Paxillus involutus mixed EST
                     library"
                     /mol_type="mRNA"
                     /strain="ATCC200175(P.involutus);
                     Skuleskogen,Skogsforsk,Sweden(B.pendula)"
                     /db_xref="taxon:231415"
                     /tissue_type="Ectomycorrhizal root tissue"
                     /clone_lib="SAMN00173256 (FHIG:A) Ectomycorrhiza plate
                     culture"
                     /dev_stage="25 days of growth after transfer/synthesis"
                     /lab_host="Escherichia coli BM25.8"
                     /note="Vector: pTriplEx2; Site_1: SfiI; Site_2: SfiI; This
                     EST clone is originating from one of three cDNA libraries,
                     constructed for transcript profiling of the mycorrhizal
                     interaction between the basidiomycete Paxillus involutus
                     and Betula pendula (birch). One library represents the
                     developed and functional mycorrhizal root tissue
                     ('(FHIG:A) Ectomycorrhiza plate culture'), a second
                     library represents axenically grown fungus ('(FHIG:B)
                     Axenic plate culture') and a third library represents
                     axenically grown plants ('(FHIG:C) Axenic plate culture').
                     Libraries were analyzed in parallel and 3555 (FHIG:A),
                     3964 (FHIG:B), and 2532 (FHIG:C) high-quality (PHRED 20)
                     ESTs of >99bp have been deposited. The cDNA libraries were
                     constructed from total RNA using the SMART cDNA library
                     Construction kit (#K1051-1, Clontech, Palo Alto, CA, USA)
                     according to the manufacturer's instructions. Full-length
                     cDNAs were trimmed by SfiI, fractionated and directionally
                     ligated into (lambda)TriplEx2 arms. The lambda library was
                     converted to a plasmid library via site-specific
                     recombination at loxP sites in a Cre+ strain (E. coli
                     BM25.8). Plasmid clones were randomly collected and
                     analysed by DNA sequencing using a plasmid-specific
                     forward primer (P104)."
BASE COUNT           78 a           58 c           79 g           76 t
ORIGIN      
        1 ctttttggac tgcccatcag cttcagtgtt gttcgtaacg ctttcttcgg tggttgggcc
       61 gagaccattg agctcgacat tcctcctcat cgcagcagta gcggataggg agagatcgtt
      121 ggcaatgacg aatcttgagg attgtcagaa atggaaaggg tggtgtactt cgggaacaaa
      181 cctcacaaga ggtattccct tggcataccg gatggagtcg taactccagt tgctgaaagt
      241 gcctcgagaa ggacgaattt atatggttga tactttggaa aaaaaaacaa a
//