LOCUS AP019719 6059 bp DNA circular BCT 12-JUN-2019
DEFINITION Clostridium butyricum NBRC 13949 plasmid pCBU3 DNA, complete
genome.
ACCESSION AP019719
VERSION AP019719.1
DBLINK BioProject:PRJDB8042
BioSample:SAMD00169824
KEYWORDS .
SOURCE Clostridium butyricum
ORGANISM Clostridium butyricum
Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
Clostridium.
REFERENCE 1 (bases 1 to 6059)
AUTHORS Miura,T., Furukawa,M., Shimamura,M., Ohyama,Y., Yamazoe,A. and
Kawasaki,H.
TITLE Direct Submission
JOURNAL Submitted (10-JUN-2019) to the DDBJ/EMBL/GenBank databases.
Contact:Director-General Biological Resource Center
National Institute of Technology and Evaluation, Biological
Resource Center, NBRC; 2-5-8 Kazusakamatari, Kisarazu, Chiba
292-0818, Japan
URL :https://www.nite.go.jp/en/nbrc/
REFERENCE 2
AUTHORS Miura,T., Furukawa,M., Shimamura,M., Ohyama,Y., Yamazoe,A. and
Kawasaki,H.
TITLE Complete genome sequence of Clostridium butyricum NBRC 13949
JOURNAL Unpublished (2019)
COMMENT Annotated at DFAST https://dfast.nig.ac.jp/
##Genome-Assembly-Data-START##
Assembly Method :: Unicycler v. 0.4.4
Genome Coverage :: 315x
Sequencing Technology :: Illumina MiSeq; ONT GridION
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6059
/culture_collection="NBRC:13949"
/db_xref="taxon:1492"
/mol_type="genomic DNA"
/organism="Clostridium butyricum"
/plasmid="pCBU3"
/strain="NBRC 13949"
/type_material="type strain of Clostridium butyricum"
CDS 270..581
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42440"
/product="hypothetical protein"
/protein_id="BBK79236.1"
/transl_table=11
/translation="MKQDALITGVKKYNFKNNDGENVKGAKISYLIDESDTDGENITG
YLPRQASIKDMSLISGLVELPGVYEINFKTVSGANNKEELKIIGIDFQKSCDFKNVFV
G"
CDS 587..766
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42450"
/product="hypothetical protein"
/protein_id="BBK79237.1"
/transl_table=11
/translation="MDSDFFELDSEQEVTTGSAVDISLKLDKVNDLLLIQNIFILSIL
VFFMLKYVMGGYLKK"
CDS 763..912
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42460"
/product="hypothetical protein"
/protein_id="BBK79238.1"
/transl_table=11
/translation="MISNVEFFSYVFPRLVQSAFVMGISMFSLAFIFRFGIDLLLSVF
KIIAK"
CDS 931..1191
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42470"
/product="hypothetical protein"
/protein_id="BBK79239.1"
/transl_table=11
/translation="MTKLKKILYCGGTGVIASALTTVSAFAAEGSDSSTVITGITDGL
TSGKTEFVTALAAISGIAIGFFVVKFVVKQGIKYFSTIANKG"
CDS 1280..2584
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42480"
/product="hypothetical protein"
/protein_id="BBK79240.1"
/transl_table=11
/translation="MKKIMKRFLILFLCFSLLSGIYTKKANAIAVVDDAFYIVAGVVV
TGATIYALVDHTLSMGSSSHTANDSANISSSLSNTQKLSLYLTVKTLENGSKFLTWTK
SGLDWLTNFIDTTINTSYVNNYGSIQISQSNTVTSPLVLSSQFTDQSCVFRYDIGYGQ
TSRSLYYGSFAGPYYVGAFFYNYNYYIGVFNANENVYSLPVSSVVNSFEVEFPERSCD
VKDYSPDVVDYNFFNPEPEPDEDNSYKPKIGFPLREYDKNKDGYEPVINPDTGEEYEP
YETPYTGDDIKENPDILVIPDGSTNPDPNPEPTPDPNPEPTPDPDPNPDTGNDEDKLP
ENDTPVLDFSPLYGATRKFPFCLPWDIYDCIKVFKSEEEPFKYEFKETKYSDTEIIII
PNFEIDFSKIPYIDLMRNILKLFLYYQFIRFLMISIRKVMWS"
CDS 2584..2844
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42490"
/product="hypothetical protein"
/protein_id="BBK79241.1"
/transl_table=11
/translation="MYSFIIKTINALIRGLGAVLTFIFKVLPDSPFQKHVLENEVIMP
YLKMINYFVPVAEILITFQALLIAIGVYYIYQVVARWVKIIS"
CDS 2857..3558
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42500"
/product="hypothetical protein"
/protein_id="BBK79242.1"
/transl_table=11
/translation="MISFYSGTPGSGKSLKMAKEVVMWLKTFKKNVIANVEIDRDYIL
KGKQGGEFFYLANEEFSPDYFYEYAIKYHDIGKEHQTLIVVDEAQTIFSPSAVKLFSQ
KEPRYRQNWLDFFTQHRHLGFDIILISQFDRLIDPQVRCLFEYNFVHRKANNFGLLGQ
IFTLMHISLFVQIQKWYGMNEVCSKTFYTYSKKYAKIYNSYAFREKVIEILTQKYGSK
KMEELMGFKNAKKVS"
CDS 3749..3898
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42510"
/product="hypothetical protein"
/protein_id="BBK79243.1"
/transl_table=11
/translation="MRVNISIPDEVKQFFEDYSKKTGVPQSSLMALALSEYKDKIERS
LSNDK"
CDS 3888..4583
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42520"
/product="hypothetical protein"
/protein_id="BBK79244.1"
/transl_table=11
/translation="MTNNEIIDANKLSQLTSSIGSIKLEMRTCAMSFFRIGEKLFNIQ
ESEVYKIKGYKSFVSFCKGEFGISKVQSYRFVGIYKKFSSERYSSFTYSQLIEMLSLK
DSDIDKVNPGMTVKEIRTLKKDLKESKEKEDIQFGEQTVVDIQESEVVEVPEKTDKEI
IEEKNLRISNQELLIQRLYERLRIESKEKDKKIEKLSEELKETLNELKALKEKKNREI
HKLRTKLKEQSNN"
CDS 4810..5898
/codon_start=1
/gene="repB"
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/inference="similar to AA sequence:RefSeq:WP_010965177.1"
/locus_tag="Cbu04g_42530"
/product="replication initiation protein"
/protein_id="BBK79245.1"
/transl_table=11
/translation="MSLKDSLKKAEIDKDLLKDIINNIEYNKTIIKYYERFSKESSIV
FKNTILENKRDSISFCNKYWLLDKYEKHKIKDFKKTNLCHDKFCSNCKKVKQASRMAK
YIPQLEQYKGQLYHLTLTLPNCSGNDLRLTIKHMSKCFLRLVQFLDGRKKIRGINFGQ
AFQGAIRSLEVTFKNDNYHPHYHVALVLNNFKMTDKKYKNKYSYNNKHGIKELTRLFS
AEEILIQKIWYLLINNITVTRQNIEALKDGYSCCMEKFSENDYAELFKYMTKVTGEDG
FTLTYENFVALYYGLYRIKQIQGYGVLYNITDDGDLEKLEEEYENYIEQLKQKESPVA
SYEAPQDLIKDTEYMLISRKSFFKYLNE"
BASE COUNT 2201 a 684 c 967 g 2207 t
ORIGIN
1 tttccttgct aattcttctt gagttaatcc tttcttaatt ctcatttctt taagtgtcat
61 tttctttctc cttaaaatct aattttaccc ctaatcaaat tttaatttaa ttataatgga
121 tattgtaaaa atgtccagtt acttcaatac tagtaaaaaa atgatggtaa ttgtctacta
181 gtttttattt actagtacaa aattactagt tataataagt atgtaaataa gtaacgacat
241 cgttactaaa aaaaatggag gtattattta tgaaacaaga tgctttaata actggggtta
301 aaaagtacaa ttttaaaaac aatgatggtg aaaatgttaa gggtgctaaa ataagttatc
361 taattgatga atcagacacc gatggtgaaa atattactgg ttacttacct cgtcaagcaa
421 gtattaaaga tatgtcttta atttctggtt tagttgaact tccaggagtt tatgaaatta
481 attttaaaac tgtttcagga gctaacaaca aagaagaatt aaaaataatt ggtatagatt
541 ttcagaaatc ttgtgatttt aaaaatgttt ttgttggtta attaaaatgg atagcgattt
601 ttttgaatta gattctgaac aagaagtaac tacaggttca gcagttgaca ttagtttaaa
661 gttggacaag gtcaatgatc tattgttaat tcaaaatatt tttatcttat cgattttagt
721 attttttatg cttaaatacg taatgggagg atatttaaaa aaatgatatc taatgttgaa
781 tttttttctt atgtttttcc tagacttgtt caaagtgctt ttgtaatggg tattagtatg
841 ttttctttag cgtttatatt ccgttttgga attgacttac ttttatctgt ttttaaaata
901 atagcaaaat aaaaaattgg aggtaatgta atgactaaat taaagaaaat tttatattgt
961 ggtggtactg gtgttattgc aagtgcatta acaactgttt cagcttttgc agcagaggga
1021 agtgatagct caacagttat aactggaata actgatggtt taacttctgg aaaaactgaa
1081 tttgtaactg ctcttgctgc tatttctgga attgctattg gattctttgt tgtaaagttt
1141 gttgtaaaac aaggtattaa gtacttttct actattgcaa ataaaggcta ggattaatta
1201 taaaaaaaga ttttcggcat attggcgcag gcctttaaat gtgccgaaaa tcttttttta
1261 ttttaaatag aggtgttata tgaaaaaaat tatgaaaaga tttttaattt tatttttatg
1321 ttttagttta ttaagtggaa tttatacaaa aaaagcaaat gctatagccg tagttgatga
1381 tgctttttat atagttgccg gtgttgtagt tactggtgct acaatttatg ctttagttga
1441 ccatactctg tctatgggtt caagttctca tactgcaaat gattctgcaa atatatcttc
1501 ttctttatct aatacacaaa agttatcact ttatttaact gttaaaacat tagaaaatgg
1561 ttctaaattt cttacttgga ctaaaagtgg actagattgg cttactaact ttatagatac
1621 tacaataaat acatcttatg ttaataatta tggttctatt caaatttcac aatctaatac
1681 tgttacttct ccacttgtgt taagttctca atttacagac caaagttgtg tatttagata
1741 tgatattggt tatggtcaga cttccagaag tctttactat ggttcttttg ctggacctta
1801 ttatgttggc gctttttttt ataattacaa ttattatatt ggtgttttta atgcaaatga
1861 aaatgtttat tcacttcctg tttcaagcgt tgttaattca tttgaggttg aatttccaga
1921 gcgttcatgt gacgttaaag attattctcc agacgttgtt gattataatt tttttaatcc
1981 agagcctgaa ccagatgaag ataatagtta taaacctaaa atcggttttc cattacgtga
2041 atatgataaa aataaagatg gatatgagcc agttattaat ccagatactg gcgaagaata
2101 cgaaccatat gaaactcctt ataccggtga tgatataaaa gaaaatccgg atattttagt
2161 gattccagat ggttctacta atccagatcc aaatccagaa cctacaccag acccaaatcc
2221 agaacctaca ccagacccag acccaaatcc agatacggga aatgatgaag ataaacttcc
2281 tgaaaatgat actccagttt tagatttttc tccactttat ggagctactc gaaaatttcc
2341 tttttgttta ccatgggata tttatgattg tattaaagtt ttcaaaagtg aagaagaacc
2401 atttaaatac gaatttaaag aaactaaata ttcagacacg gaaattatta taattccaaa
2461 ttttgaaata gatttttcaa agatacctta tattgattta atgagaaata ttttaaaatt
2521 attcctttat tatcaattta tacgtttctt aatgataagc ataagaaagg tgatgtggtc
2581 ttaatgtatt cttttataat aaaaactata aatgctttaa tacgtggatt aggagctgta
2641 ttaactttta tttttaaagt tttaccagat tcaccttttc aaaagcatgt tttagaaaat
2701 gaagttatta tgccttattt aaaaatgatt aattattttg tccctgtcgc tgaaatatta
2761 ataacttttc aggctctttt aattgctatt ggtgtttatt atatttatca agttgttgct
2821 cgttgggtaa aaattattag ttaggagttt ttttatatga taagttttta tagtggtacg
2881 cctggaagtg gtaaatcttt aaaaatggca aaagaagttg ttatgtggtt aaagacattt
2941 aaaaagaatg taatagcaaa cgttgagata gatagagatt atattttaaa aggtaagcag
3001 ggtggagaat ttttctacct tgctaatgaa gaattttctc cagattattt ttatgagtat
3061 gcaattaaat atcatgatat tggtaaagaa catcaaacac ttattgtcgt tgatgaagca
3121 cagactattt tttcccctag tgcagtaaaa ttatttagtc aaaaagaacc tagatatcgt
3181 caaaattggt tggatttttt tacgcagcat agacatcttg ggttcgatat aattttaatt
3241 agtcaattcg atagattaat agatccacaa gtaagatgtt tatttgaata taattttgta
3301 cacagaaaag cgaataattt tggattgctc ggacaaattt ttactcttat gcatattagt
3361 ttatttgttc aaattcaaaa atggtacggt atgaatgaag tatgtagtaa aacattttat
3421 acttattcta agaaatacgc taagatatac aattcatatg cttttagaga gaaagtaatc
3481 gaaatattga cacagaaata tggttctaaa aaaatggaag agttaatggg gtttaaaaat
3541 gctaaaaagg tatcttaata aatatgagtt ttatggttta gttattgata tattcttaat
3601 actgtatgat ttttgtctta tagtaaaata tttctaaaaa catttgacat ttcagcatca
3661 ttattatatc atataaatat caagttgatg tttcacgtga aacatttaat agtaacgacg
3721 tcgttacaaa atatttggag gtatttatat gagagttaat atttcaatcc cagatgaggt
3781 taaacaattt tttgaagatt atagtaaaaa aacaggagtt ccacaaagtt cacttatggc
3841 tttagctctt tctgagtata aagataagat agaaaggagt ttatctaatg acaaataatg
3901 agattattga tgctaataag cttagtcagt taactagttc tataggttct attaaattag
3961 aaatgagaac atgtgctatg tctttttttc gcataggaga aaaactcttt aatattcaag
4021 aaagtgaagt ttataagatt aaaggatata aaagtttcgt ttctttttgc aaaggtgagt
4081 ttggaatttc taaagttcaa tcttatcgtt ttgttggtat ttataaaaaa tttagttctg
4141 aaagatactc tagttttact tattcacaat tgatagaaat gctttctctt aaagattctg
4201 atatagataa agttaatcca ggtatgactg ttaaggaaat acgaacatta aaaaaagatt
4261 taaaagaatc taaagaaaaa gaagatattc aatttggaga acaaactgtt gttgacattc
4321 aagaaagtga agttgttgaa gttcctgaaa aaactgataa agaaattata gaagaaaaaa
4381 atctcagaat ttcaaatcaa gaacttttaa tacaaagatt atatgagcga ttaagaattg
4441 agagtaaaga gaaagataaa aaaatagaaa aattatctga agaacttaaa gaaactctaa
4501 atgaactgaa agctcttaaa gagaagaaaa acagagaaat acataagcta agaactaagc
4561 ttaaagaaca aagtaataat taaagattac aactcttctt tccctaaaag attacacttt
4621 ggcagacctt gtgtcggcaa tgcttatgct tataagttaa cgaatagtgg aatatatgtt
4681 gttagtggta cgggcatcgc agatagtagc gtagcgtgcc attaactttt taaaattttc
4741 tttctatctt gatatattag aaataagtta acccataaaa aaaatttact ttagaaaagt
4801 ggtgataatt tgagtttaaa ggattctttg aaaaaagctg aaatagataa agatttatta
4861 aaagatatta ttaataatat tgaatataat aagacaataa ttaaatatta tgaacgtttt
4921 tctaaggagt caagtatagt atttaaaaat actatattag aaaataaaag agattctatt
4981 tctttctgta ataaatattg gcttttagat aaatacgaaa agcataagat aaaagatttt
5041 aagaagacta atttatgtca tgataaattt tgtagtaatt gcaaaaaggt taaacaagct
5101 tccagaatgg caaaatatat cccgcaactt gaacagtata aaggtcagtt gtatcattta
5161 actttaacat tacctaattg ttctggaaat gatttaaggt taactataaa acatatgtct
5221 aaatgttttt taagacttgt acagttttta gatggtagga agaagatacg tggtataaat
5281 tttggtcaag cttttcaagg agcaataagg tctttagaag ttacgtttaa aaatgataac
5341 tatcatccac attatcacgt tgcattagta ttgaataatt ttaaaatgac tgataaaaag
5401 tataagaata aatattctta taataataaa catggtatta aagagttgac taggcttttt
5461 tcagcagaag aaattttaat tcaaaagata tggtatttat taataaataa tataactgtt
5521 actagacaaa acattgaagc tcttaaagat ggatattctt gttgcatgga aaaattttca
5581 gaaaatgatt atgcagagct ttttaaatat atgacaaagg ttactggtga agatggtttt
5641 acattaactt atgaaaattt tgttgcttta tattatggac tttatagaat taaacagatt
5701 caaggatatg gagttttgta taatataact gatgatggag atttagaaaa attagaagaa
5761 gagtatgaaa attacattga acagttaaaa caaaaagaaa gcccagtggc aagttatgaa
5821 gctccacagg acttaataaa agatactgaa tacatgttaa tttctcgaaa gtcatttttc
5881 aagtatctca atgaataatt ttattataat acaaggggta gaaaatttct atctctttta
5941 ttttaaaaga aaatttcttc aatagttttt tcgagtgcat tagctatttt tcgtgctgta
6001 tctaaattac aagtattttt tctttctgct tttactatag tgcttaatga tagttctgt
//