LOCUS AP019718 8060 bp DNA circular BCT 12-JUN-2019
DEFINITION Clostridium butyricum NBRC 13949 plasmid pCBU2 DNA, complete
genome.
ACCESSION AP019718
VERSION AP019718.1
DBLINK BioProject:PRJDB8042
BioSample:SAMD00169824
KEYWORDS .
SOURCE Clostridium butyricum
ORGANISM Clostridium butyricum
Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
Clostridium.
REFERENCE 1 (bases 1 to 8060)
AUTHORS Miura,T., Furukawa,M., Shimamura,M., Ohyama,Y., Yamazoe,A. and
Kawasaki,H.
TITLE Direct Submission
JOURNAL Submitted (10-JUN-2019) to the DDBJ/EMBL/GenBank databases.
Contact:Director-General Biological Resource Center
National Institute of Technology and Evaluation, Biological
Resource Center, NBRC; 2-5-8 Kazusakamatari, Kisarazu, Chiba
292-0818, Japan
URL :https://www.nite.go.jp/en/nbrc/
REFERENCE 2
AUTHORS Miura,T., Furukawa,M., Shimamura,M., Ohyama,Y., Yamazoe,A. and
Kawasaki,H.
TITLE Complete genome sequence of Clostridium butyricum NBRC 13949
JOURNAL Unpublished (2019)
COMMENT Annotated at DFAST https://dfast.nig.ac.jp/
##Genome-Assembly-Data-START##
Assembly Method :: Unicycler v. 0.4.4
Genome Coverage :: 315x
Sequencing Technology :: Illumina MiSeq; ONT GridION
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..8060
/culture_collection="NBRC:13949"
/db_xref="taxon:1492"
/mol_type="genomic DNA"
/organism="Clostridium butyricum"
/plasmid="pCBU2"
/strain="NBRC 13949"
/type_material="type strain of Clostridium butyricum"
CDS complement(210..494)
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42360"
/product="hypothetical protein"
/protein_id="BBK79228.1"
/transl_table=11
/translation="MKKESSIKNLIKANNEQNKNANNVSNIVITTYIILLIFLYYLLK
HNISYELFSILFVGQLSKSFYKYRISKNIIYLYESIGCIIAIVGLIVLMI"
CDS complement(523..1476)
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42370"
/product="hypothetical protein"
/protein_id="BBK79229.1"
/transl_table=11
/translation="MIKKISKITSYILFAALTTSLLPSINANAAQSNESSIINYIEST
TGTDDISSNIEETKNYIAVSDGENSTIKIPRDSSNPITLTPAYNEDQSFGIKLPLESS
DSNISSNGTVLYTSDDSSVSFGAQPIHADGLEGVRTLVTINDENASKNYTFSFDLEEG
SKLVTDAEYLGKEYSTGEIFIVNNQNIITGIIDKPWAYDANGHKVSTKYKIKDENKLV
QTINFSKNTTFPVVADPSAWQITKCAGSIAWALGSGIFAGAKLLKIKKYIKALGGVKE
AAALLLGATTWAEKMEAGGSALVNLAAEISGVKDIKENCFS"
CDS complement(1478..1957)
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/inference="similar to AA sequence:RefSeq:WP_003723013.1"
/locus_tag="Cbu04g_42380"
/product="thiol reductase thioredoxin"
/protein_id="BBK79230.1"
/transl_table=11
/translation="MSFKQVKIKYLIIPFIILFLLTGLFVVNNYYKNTLESNSKISSF
SCKNLLLDISQDALNEKILNKESFFIYIGRPTCPDCKTFMPKLENILSETNKSIFYYN
TEATASKKQEIRDYLHLLNVKSVPSILLFSNGNIEQIYDCQDKNQVENFLEQFKGDF"
CDS complement(2134..2367)
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42390"
/product="hypothetical protein"
/protein_id="BBK79231.1"
/transl_table=11
/translation="MVDNKEKITKLDEKIKQLQAQKNSLIAREKEKERKARTKRLIEI
GAIFASIGFDTVEKANAFKSQFNNENVKTESFD"
CDS 2635..4278
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42400"
/product="hypothetical protein"
/protein_id="BBK79232.1"
/transl_table=11
/translation="MSIYHFGQMKVISRGTGRSVIASSAYISGEKLYNEYDGLTHDYT
RKQGVVFSEVMLPENAKDEWKNRQILWNEVEKIEKSKVSQLARSFEVGLQTEFTLEEN
IKLIKEYVKDNFIDKGMCADICIHDKSDGNPHAHVMLTMRKIDEQGKFLPKAEKQYLC
RNDKGDEKYLNSNDLKDDRDFGKVYKCRYKNDYKELTNRELKMEEYKNYKKISKYPLD
KKIDMCSDWNNTNNVELWREDWARINNRLFDEKGLNIRIDHRSYERQDINRVPTIHEG
YGARLRAKNGKECDRIEINRYIANLNEKIKGYENDIHKLKNEMIELNRDMDVKMKAGR
EEISLERPKSSYKTTDSDIKEILKKLKEKLSDKANKHLGVDRALKYIDKRKIKTNNDV
KVIKSQLATIRNNYGNRIDVIDDYINRMNSIYDNILRKSREVNSLEKEVGELGIFKFK
QKKYLNERIEELKNEINGFKKSEFYQKDIKYTDKIEELKGQRKECLEVLKKCDNENSI
LYDIETLSKDKNKLLGNLNKDRNNDINFKVKRHSNELEL"
CDS 5134..5433
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42410"
/product="hypothetical protein"
/protein_id="BBK79233.1"
/transl_table=11
/translation="MKTCKNCKEFIKDTEICKIHSLMIHDKTVATYCSKYNESRCLHK
GKVQCINCSKMNRYGWCAIKMRCFTEEEQKKERTCIKYYARSFKKAHVKKSKKKK"
CDS 5998..6588
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/locus_tag="Cbu04g_42420"
/product="hypothetical protein"
/protein_id="BBK79234.1"
/transl_table=11
/translation="MDYKLMELDDLAEYINLQLEKGRNFTEVAGEDFSCNESTLRKRL
TAKKLYKRIGNKYVRQCQTIDDRSKSSDGNNNVRQSVRQDVTDGNIAEITGDIKSQSI
TVSPSDKEKFLGLISNYELIMNMLDNYKNNEDKFNRANGLVVELPVEKKKDFRITLRL
NDVVYEEFKEFADKNKQFTVKELVSQALKEFIQKYK"
CDS 6681..6935
/codon_start=1
/inference="COORDINATES:ab initio
prediction:MetaGeneAnnotator"
/inference="similar to AA sequence:RefSeq:WP_011860873.1"
/locus_tag="Cbu04g_42430"
/product="hypothetical protein"
/protein_id="BBK79235.1"
/transl_table=11
/translation="MDKEMLEALRAMFKEELKAELEPIKADIKEIKNKMDSVYDQTAD
LTEFRTETKQGISDIQRNLNTMELVTSKNWNDIAQLKSIK"
BASE COUNT 3336 a 872 c 1339 g 2513 t
ORIGIN
1 taaaaaatga aatagaacag ttaaagagta aaccgaaggc tacaagaaaa acagaaaagt
61 agaaatttaa cttaattatt ataaatacta aacaaaagca acgattttaa tgcttataaa
121 tggcttaaaa tcgttgcttt atttttagtt atttcgctaa ataatatttt tacgaaattt
181 ttgataaaat aggattggta tataataaat taaatcatta atactattaa tccaactatt
241 gcaatgatac aaccaataga ttcgtataaa taaatgatat ttttacttat tcgatattta
301 taaaatgatt tagaaagttg accaacaaat agtatactga ataactcata tgatatatta
361 tgttttaata gataatatag aaaaatcaat aatataatat atgttgttat aacaatgtta
421 ctaacattgt tagcgttttt attttgttca ttattagctt ttattagatt ttttattgaa
481 gattcttttt tcatatatta ctcctaaatt atatttagat atttaagaaa aacaattttc
541 tttaatatct ttaacacctg agatttcagc agccaaatta accaatgcac ttccacccgc
601 ttccattttc tctgcccagg ttgtggcacc gagtaataat gcagcagctt ctttgacccc
661 acctaatgct ttaatatact ttttaatttt taataattta gctccagcaa agatacctga
721 accaagcgcc catgcaatag aaccagcaca ttttgttatt tgccatgctg atgggtcagc
781 aactacaggg aaagtagtat ttttgctgaa attaatggtt tgcactaatt tattttcatc
841 ttttatttta tattttgtag agactttatg accatttgca tcatatgccc aaggcttgtc
901 aataatacct gtaataatat tttgattatt aacaataaaa atttcaccag ttgaatattc
961 tttacctaaa tattcagcat cagttactag tttagatcct tcttctaaat caaaagaaaa
1021 agtatagttt ttggaagcat tttcatcatt aatagtaact aaagttctaa ctccttctag
1081 cccatcagcg tgtattggtt gagcaccaaa tgaaactgaa gaatcatcac ttgtataaag
1141 tacagttcca ttactactaa tattggaatc agatgattct aatggtaatt ttataccaaa
1201 agattgatct tcattataag caggagtaag agtaattgga tttgatgaat ctctaggaat
1261 tttaatagtt gaattttcac catcagatac ggctatatag tttttggttt cttctatatt
1321 tgatgagata tcatcagttc cagtagtact ttcaatataa ttaatgattg atgattcatt
1381 agattgagct gcattagcgt ttatacttgg aagtaatgaa gttgttagag cagcaaataa
1441 aatatatgat gttatttttg aaattttttt tatcattcta aaagtctcct ttaaattgtt
1501 ctaagaaatt ttcaacttga tttttatctt gacagtcata tatttgttca atattaccat
1561 tactaaaaag caatatactt ggtacagatt taacattaag caaatgtaga taatctcgta
1621 tttcttgttt tttacttgct gttgcttcag tattataata aaatatcgat ttatttgttt
1681 cagatagtat attttctaat tttggcatga aagttttaca atcaggacaa gttggtctgc
1741 ctatataaat aaaaaagctc tctttattta atattttttc atttaaagca tcttgactta
1801 tatctaataa taaattttta catgaaaaac tagatatttt agaattagat tctaatgtgt
1861 ttttataata attatttaca acaaataatc ctgttaataa aaacaaaatt atgaacggaa
1921 ttattaaata cttgattttt acttgcttaa aactcaaaat ggaccctcat ttctattaca
1981 ttgttttttg ttaaggttga ctttacataa ttatccggat aataagagat attaaattaa
2041 gtctataaat gttctataca tccaatataa cataaaatga attaaatgta aaaaaaaata
2101 aaatattcca atttattgct atattttata aaatcaatca aatgattcag tttttacatt
2161 ttcattatta aattgtgatt taaaagcatt ggctttttca acagtatcaa atccaatact
2221 agcaaatata gcaccaattt caattaatct ttttgtacga gctttacgct ctttttcttt
2281 ttctctagca attaatgaat ttttttgggc ttgaagttgt tttatttttt catcaagttt
2341 agttatcttt tctttattat caaccatgta aatcacacct ttctataaat attataagta
2401 tgtaaaaata tgttttcaac tacaggaata ataagcttaa gaaaaatata taaatattgt
2461 aaaaatttta aattaacgat agaattatga gtatgggcaa tcccgtagtg aaaactgaag
2521 ggcgcactta taccccactt tgggaaagtg ggagtgcggt cttttcagac agaactcttg
2581 cagagggcaa acgaaaaatt aaaaattttt cttatatagt aaggtggtat agaaatgtca
2641 atttatcatt ttggacaaat gaaagtaata agcagaggaa caggaagaag tgtaattgca
2701 tcaagtgcat atataagtgg tgaaaaatta tacaatgaat atgatggact tacacatgat
2761 tatacaagaa aacaaggagt cgttttttca gaagtaatgt tacctgaaaa tgcaaaagat
2821 gaatggaaga atagacagat attatggaat gaagttgaga aaatagaaaa gagtaaagta
2881 agccagttag cacgaagttt tgaagttggt ttgcagacag aatttacact tgaagaaaat
2941 ataaagttaa ttaaagaata tgtgaaagat aattttatag ataagggaat gtgtgcagat
3001 atctgtatac atgataagag tgatggaaac cctcatgctc atgtaatgct gactatgaga
3061 aaaattgatg aacagggaaa atttttaccc aaggcagaaa agcaatatct atgtagaaat
3121 gataagggtg acgaaaaata tcttaattcc aatgatctta aagatgatag agattttgga
3181 aaagtatata agtgcagata taagaatgat tataaagagt taactaatag agaattgaaa
3241 atggaagagt ataagaatta taaaaagatt tctaaatatc cactagataa aaaaatagat
3301 atgtgttctg actggaataa taccaataat gtagagctat ggcgtgaaga ttgggcaaga
3361 ataaataata gactatttga tgaaaaagga ttaaatataa gaatagatca tagaagctat
3421 gagaggcagg acattaacag agtaccaaca attcatgaag gatatggggc aaggcttaga
3481 gctaagaatg gaaaagaatg tgacagaata gagatcaata gatacatagc aaatctaaat
3541 gagaagatta aagggtatga aaatgacatt cataaattaa aaaatgaaat gatcgaatta
3601 aatagagaca tggatgtcaa aatgaaggct ggcagagaag aaataagttt agaacgtcct
3661 aaaagctcat ataagacaac agatagtgat attaaagaaa tactaaagaa actaaaagaa
3721 aagctgtcag ataaagcaaa taagcattta ggagtagaca gagcattaaa gtacattgat
3781 aagagaaaaa taaaaacaaa taatgatgtg aaagtaatta aatctcaact agcgactata
3841 agaaacaatt atggaaaccg gattgatgta atagatgatt atattaatag aatgaattca
3901 atttatgata atattttaag aaagagtaga gaggtcaatt cattagaaaa ggaagttgga
3961 gagcttggaa tatttaagtt taaacagaaa aagtatttga atgaaagaat tgaagaatta
4021 aaaaatgaaa ttaatggatt taaaaaatca gagttttatc aaaaggatat aaaatatact
4081 gacaaaatag aagaactaaa aggacagaga aaagaatgtt tagaagtctt gaaaaaatgt
4141 gataatgaaa atagcatatt atatgatatt gaaacattga gtaaagataa gaataagctc
4201 cttggcaatt taaataagga tagaaataat gatattaatt ttaaggtgaa aagacattca
4261 aatgaactag agctttaatt tagaatatgc cttttgaggt cagaagtata aaaataattc
4321 atttcttgta aattgcaagg gctacgcctt caaaaaaata atggccgcag catgatcttc
4381 ttcgaagaga tgcagcagcc attatttttt tgaacaattg acaattcatt tcttattttt
4441 tattaagtga tagtcaaaag gcataacagt gctgaataga aagaaattta cagaaaagaa
4501 aattatagaa tttagtatga ttaattatac tcatttatga atgtttaatt gaatacaaaa
4561 aaaaatactt gttatgtatt caattacggg ttaaaatata gacaagttga aaaatttaat
4621 aaaaaaataa gtcctcagct cttatatatt aagctaccaa cttagtatat aagccaaaac
4681 ttaaatgtgc taccaacaca tcaagccgtt agagaactct atctatagca atatttcaaa
4741 tgtaccgaca tacaagagaa acattaacta tatatattca atttatgaga ttatcttaac
4801 agatataaat gtaaattgca ataagtaaga tttagaagtt tatagccttt gtgtattgga
4861 agcagtacgc aaaggctttt ttatttgata aaaattagaa gtatatttat tttttcataa
4921 ttaatttatg aaaatgaaag ggggtgagca aagtgacaga ggaaagcagt atcttatcaa
4981 ataacaaggt attagcaata tcattattga ctttagcagt aaacattatg acttttatag
5041 tgcttgtagc taagtagtac gaaaggggga gctttaaaaa gctccttgga atacatagaa
5101 ttcataaatt aatttatgaa aagaagggcg tatatgaaaa cttgtaaaaa ttgcaaagag
5161 tttattaaag atactgaaat atgcaaaata cattcgttga tgattcatga taaaacagta
5221 gcaacctatt gcagtaaata caatgagtca agatgtttac ataaagggaa agtccaatgt
5281 attaattgtt caaagatgaa ccgatatgga tggtgtgcca taaaaatgag atgttttaca
5341 gaggaagaac agaaaaaaga acgtacatgc attaaatatt atgcaaggag ctttaaaaaa
5401 gctcatgtaa agaagagtaa aaagaaaaaa taatttattt attaatttaa tattgagagt
5461 gccgacacag tatgcactaa aaaatatatc tgtggtgtag tgagccgata caaaaggata
5521 gtcactcgca ttttcataat acatcttatg ttatgattat gtgtcggtgg gacttcacga
5581 cgaaaaccca caataaaaaa agagttcggg gtagggttaa gcatagttga ggcaactaaa
5641 caatcaagct aggatatgca gtagcagacc gtaaggtcgt tgtttaggtg tgttgtaata
5701 catacgctat taagatgtaa aaatacggat accaatgaag ggaaaagtat aatttttgga
5761 tgtagtttgt ttgttcatct atgggcaaac tacgtccaaa gccgtttcca aatctgctaa
5821 aaagtatatc ctttctaaaa tcaaagtcaa gtatgaaatc ataaataaag tttaattttg
5881 aagttattat gatattatgt ttttctatta aaataaatta agtatataga atagtttaat
5941 aatagtatat acttaatgtg ataagtgtct gacagtgtca cagaaaggat gattgttatg
6001 gattataagc ttatggagct agatgatcta gcagagtata ttaatttaca gttagaaaag
6061 ggaagaaact ttactgaagt agctggggaa gatttttcat gtaatgaaag tacacttaga
6121 aagagattaa cagcaaagaa attatataaa agaataggca ataagtatgt gagacaatgt
6181 cagactatag atgacagaag caaaagttca gatggaaata acaatgtcag acaaagcgtc
6241 agacaagatg tcacagacgg aaatattgca gaaattactg gtgatataaa gtcgcaaagt
6301 atcacagtat caccaagcga taaagaaaag tttttgggac tgataagtaa ttatgaattg
6361 ataatgaata tgttagataa ttataaaaat aatgaagata agtttaatag agctaatggg
6421 ttagttgtag agctgccagt agaaaagaaa aaagatttta gaattacatt aagattaaat
6481 gatgtagttt atgaggaatt taaagaattt gcagataaaa ataagcagtt tacagtgaaa
6541 gaattagtaa gccaggcttt aaaagaattt atacaaaagt ataagtagaa ataaggatta
6601 atttcaaaag aataatacat taagagaata aacaatgata taataaattt actcaatatg
6661 ataatagtga ggtgatataa ttggataaag aaatgttaga ggcattaaga gcaatgttca
6721 aagaagaatt aaaggcagag cttgagccaa taaaggcaga cattaaagaa attaaaaata
6781 aaatggattc tgtatatgac cagacggcag atttaacaga gtttagaaca gaaaccaaac
6841 aaggaataag tgacatacaa agaaatttaa atactatgga attagtgaca tctaagaatt
6901 ggaatgatat tgcacagtta aagtcaataa aatagtatct ttataatatt tgagtatcaa
6961 taatggtact ctttttttta catttttata tgtcatacat ataaaataca atgtattgta
7021 ttgcaaatac atattgaaaa aagaaaaata gaagcatata ataaatatat aaactataaa
7081 tacaaataca atacagtgaa atacaaatgc aatacataaa aaatacattt gtaatataaa
7141 tgtattacaa atgaaagggg tgctgaaaat ggcagatatt aaagcaacaa gtttcagagt
7201 cagtgaagat gacatagcaa aatttaagga atttgcagat aaggaaggtt ataaccaagc
7261 cgaggcattt aaaagcataa tgcagactgt tgaaatggct aaagctaaga atatgattaa
7321 ggatagagca aaggaaatag aagtatttca ggatactata aacaatctta tgagtatgtt
7381 tttaaacagt ctcaatgtta atcagacatc agaagaaaga ataagagagg agttaagcca
7441 agagctgcag actaaagata atacaataag taatttgcaa aaacaattaa cagagaatga
7501 aaatgatata aaacgagtaa aggagctatc cgcaaaccgt tatgaagaaa ttcaaaaatt
7561 gtcagcagca ggagttaaac aagaaaatgt aaatagagaa ttacaaaaga ctattgataa
7621 attaaacagt aacaatgatt tgttgcagga acagttgaaa gaatataagc agtataaaga
7681 tgattataaa aaattaaaca atcagttgga tcagttaaaa gcagagcatg aggaattaaa
7741 gaaaaataac aataaattaa ataatgataa tgaattatta aataataaaa ttagtgctaa
7801 tagtgatatg attgaatttt ataaaaatga aattgttagt aaagataagg atatcgataa
7861 ttataaaagt gatattaagg aatcagatat taagtataac agccaaataa aagaattaga
7921 ggctaaatat aatactaaga tagaagaagt taaagaggaa catgaaaagg cgttaaatga
7981 ccagttgaga aataatatag ataacttaaa tgctaaacat gagattgaat taagtaaaaa
8041 agatttagaa atacaaaagt
//