LOCUS AP011120 2773 bp DNA circular BCT 07-OCT-2016
DEFINITION Rhodococcus opacus B4 plasmid pKNR02 DNA, complete sequence.
ACCESSION AP011120 AB193160
VERSION AP011120.1
DBLINK BioProject:PRJDA34839
BioSample:SAMD00060964
KEYWORDS .
SOURCE Rhodococcus opacus B4
ORGANISM Rhodococcus opacus B4
Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
Nocardiaceae; Rhodococcus.
REFERENCE 1 (bases 1 to 2773)
AUTHORS Sekine,M., Hosoyama,A., Kosugi,H., Yamada,R., Matsuo,Y.,
Tsukatani,N., Fujisawa,T., Takarada,H., Omata,S., Kishi,E.,
Shimizu,A., Koga,C., Saito,S., Nakazawa,H., Sekigawa,T.,
Nishiko,R., Takeyama,M., Tanikawa,S., Tago,S., Fujita,N., Kato,J.,
Na,K.S., Ohtake,H., Nagayasu,K. and Harayama,S.
TITLE Direct Submission
JOURNAL Submitted (30-MAR-2009) to the DDBJ/EMBL/GenBank databases.
Contact:Director-General Department of Biotechnology
National Institute of Technology and Evaluation, NITE Genome
Analysis Center (NGAC), Department of Biotechnology; 2-49-10
Nishihara, Shibuya-ku, Tokyo 151-0066, Japan
URL :http://www.bio.nite.go.jp/
REFERENCE 2
AUTHORS Takarada,H., Sekine,M., Hosoyama,A., Yamada,R., Fujisawa,T.,
Omata,S., Shimizu,A., Tsukatani,N., Tanikawa,S., Fujita,N. and
Harayama,S.
TITLE Comparison of the complete genome sequences of Rhodococcus
erythropolis PR4 and Rhodococcus opacus B4
JOURNAL Unpublished (2009)
REFERENCE 3
AUTHORS Na,K.S., Nagayasu,K., Kuroda,A., Takiguchi,N., Ikeda,T., Ohtake,H.
and Kato,J.
TITLE Development of a genetic transformation system for
benzene-tolerant Rhodococcus opacus strains.
JOURNAL J. Biosci. Bioeng. 99, 408-414 (2005)
COMMENT Kato,J., Na,K., Ohtake,H. and Nagayasu,K. are at Department of
Molecular Biotechnology, Hiroshima University.
Rhodococcus opacus B-4 strain was isolated as a benzene-tolerant
bacterium (Kyung-su Na et al 2005). The complete genome analysis
of R. opacus B-4 was carried out by NITE. The genome analysis
indicated that this strain has one linear chromosome, two linear
plasmids pROB01, pROB02, and three circular plasmids pKNR, pKNR01,
pKNR02. The nucleotide sequence of the linear plasmid pKNR02 was
previously determined independently by Kato et al and was
registered with the accession number AB193160, which is now merged
with this record with the permission of Dr. Kato.
The differences between the entry AB193160 and this record are as
follows.
'C' at position 1965 replaced 'T'.
'G' was deleted between positions 2576 and 2577.
This work was supported by New Energy and Industrial Technology
Development Organization (NEDO).
Please visit our web site.
URL:http://www.bio.nite.go.jp/
DOGAN ; Database of Genomes Analyzed at NITE
The database contains genome sequence data, physical maps and the
genomic and proteomic data of the microorganisms analyzed.
URL:http://www.bio.nite.go.jp/dogan/Top
The microbial genomic DNA clones used for the sequencing project
are available through the NBRC.
URL:http://www.nbrc.nite.go.jp/e/index.html
FEATURES Location/Qualifiers
source 1..2773
/db_xref="taxon:632772"
/mol_type="genomic DNA"
/organism="Rhodococcus opacus B4"
/plasmid="pKNR02"
/strain="B4"
CDS 630..1244
/codon_start=1
/locus_tag="ROP_pKNR02-00010"
/product="hypothetical protein"
/protein_id="BAD95584.1"
/transl_table=11
/translation="MREGAREQSAPPKRQNVDLEQGSRVVSCRRGVRRCVLCGRSVGR
VATPRSGITRAPGPGCSVACRGSRIVAPPYRSARCRCPGLPSPPPRPIGYALWSHYTR
GPAGGGLEGSSCITPPERHTPNAFALPEFSSTLVTAVSIVLKKPPDANPIGGFFYART
TFPVPRQNTGNATRSISHDCEESRLVYLPNTRGMKKAGTYFGPA"
CDS 1374..1964
/codon_start=1
/locus_tag="ROP_pKNR02-00020"
/product="hypothetical protein"
/protein_id="BAD95585.1"
/transl_table=11
/translation="MPASPPSNNPSSGTPASMPGRPRWSASEAARRCGVGRSTIQRAL
VAGRIPDAVETEKGWSIPLDGLLAAGFTPDRPSPPDPTLTSPPNPARGHDRAPTNNDG
EHARRIAELELALERERARAELEHARRVAAEQLAAERAERVADLRHTLRMIEAPLAEQ
LHGAPEESPVEPPVAPATSTEQAPTLFGLLGRLLNR"
BASE COUNT 525 a 920 c 850 g 478 t
ORIGIN
1 agatctcgat ggcgcggaca attcgaggct gaacggagtc agacatggct tggttccttc
61 ccgagaccga gcgggcagca tgaagcgcac gactacgcgc actgcccttc tgtctcatgg
121 ggacgaggaa gccgccggag gtgcatcacc cgcggcggct tcagtgaatg gatatgaggg
181 gcagagcgtc gagctcgcgg catgcaaaag gccggcgccg aggacggggc cggccgagta
241 ggttgccgct gatcgtttag ttgtggttgt agatcggtcg cggaagagat cgcatcgacg
301 gcggcgcgtg cgcgtagtag ccgaacatca ggcccccttc acagaagtcg aagtgactcg
361 gtctaccgca ccgcgtgccg tacccgactt accttccgca gtctgagact cagtcccgtt
421 ccccagtgct tgccttgctg tggtccaggt ccaaggcagc accgacaaga ccgaacacac
481 ctcttgcact aacagtgcgt cctgggagtt ctcaccggtt ggtgaggtct gaaagaagcg
541 tcctgtttgg tcactgggct caagcaaagg gcctcgcttt gctcggactg ttctctcaag
601 cgctctgctc aacgtcttgt ccgaccctca tgcgggaggg agcgagggag cagagcgcgc
661 cccccaaacg gcaaaacgtg gatctcgaac agggttcgag ggtggtgtct tgccgtcggg
721 gggtgcgtag gtgtgtgctc tgcggtcggt cggtcggtcg cgtcgctacg ccgcgctcgg
781 ggattactcg agcgccaggg ccggggtgtt cggtcgcgtg ccgggggagc cgcatagtcg
841 cgcccccgta ccgctctgcg cgttgtaggt gtccgggcct gccttcacca ccgccgcgac
901 cgatcgggta tgccctttgg tctcactaca cccgggggcc agccggtggg ggtctggaag
961 ggagttcgtg tatcacgcca cctgagcgac acacccccaa cgctttcgcg ctgccagagt
1021 tctccagtac cctggtcact gcagtttcga tcgttttgaa aaagcccccg gacgccaatc
1081 caataggggg ctttttctat gcccggacca cctttccagt tcccagacaa aacaccggta
1141 acgccacgcg ctcgatttcg cacgactgcg aagagtcccg gctggtgtat ctcccaaaca
1201 cccggggcat gaaaaaggcc ggcacctatt tcggtccggc ctgatctaac tttgcgcggg
1261 cacagttcat ccgcagtcat aactatgaca ggtcaacgta gtgcgacctt ttagacccgc
1321 cttttcggcg tgcctatctg ttgctgccgc cctggcccgg gcagggtaag tgcatgcccg
1381 cgagccctcc ttcgaacaac ccgtcttcgg gcacgcccgc gagcatgccc gggcggcctc
1441 gctggtccgc ttccgaggca gcccgccgct gtggagtcgg gcgctcgacc atccaacgag
1501 cgttggtcgc tggccggatc cccgatgcag ttgagacgga gaagggctgg tcgatcccat
1561 tggacgggct tctggctgcc ggcttcacac ctgaccgtcc cagtcctcca gatccaacac
1621 tgaccagccc gccgaacccc gcacgcgggc atgaccgagc acctacaaac aacgacggcg
1681 agcatgcccg ccggatcgcc gagctggagc tggcgctgga acgcgagcgt gcccgagccg
1741 agctggagca cgctcgccgg gtcgcggcgg aacaactcgc tgccgaacgc gccgaacgtg
1801 tcgccgacct ccgacacaca ctccgcatga tcgaagcacc tctcgccgaa caactgcacg
1861 gcgctccgga ggagtcgccg gtagagccac cagtagcgcc ggcgacatct accgagcaag
1921 caccaacgct gttcggcctg ctgggtcgac tgctcaaccg gtagccaccg cgcccggggc
1981 cggcgcgggc accaaagaac gacctcgcga catgaaaaag gccggcacct gttcgaaggt
2041 ccggcctgat tttgggcgca cgatctcgcc aacggcctca gcctatcagc ggcggtgtta
2101 caaaccctgt caagcacatc cacaccggcg tgtcgcgaga gcatttttcg ttggcaaggg
2161 gctgcgcagc ggcctcgcgg cagctgcacc cctcacgact accttgcccg gcaagatcgc
2221 cgaacgaggg agtagcagct cgccatgcca gacccgaaga aaacacccac gcgagcagaa
2281 accctgcgcg cctggatcac gacactgaca ccgatcgcgg tcgcgatcat caccgcagcc
2341 accgctgtga tcgtgcggtg aatcaggccc agcgcagcgc cccgacaggg acaccgcgaa
2401 acgcgggcac ggacttaggt cacctaagtc tccatcttgc tccgccggag caagacctcc
2461 tggtgcgcga ccgctcgcgc acccgcactc ctcacttacg tgaggtaagt gtctatcttg
2521 ctctccgcac aagcagccga gccgcgacca atccgatgcg accagaccgc ttggccgccg
2581 gtaacagctg ctgttaccgg cggccctccc aacagtctga ggttcagcaa gaggtgtgtc
2641 tgaaacttcg gtgtcgaagt aggcagccga cgctgcactg gtcactggaa aagctcccac
2701 acccgggaga ccaacagcgc ggcttgcagt gcgcggtaaa cgatcatcaa gatcggtgcc
2761 gctcgtcggt agg
//