LOCUS AP011119 4367 bp DNA circular BCT 07-OCT-2016
DEFINITION Rhodococcus opacus B4 plasmid pKNR01 DNA, complete sequence.
ACCESSION AP011119 AB193159
VERSION AP011119.1
DBLINK BioProject:PRJDA34839
BioSample:SAMD00060964
KEYWORDS .
SOURCE Rhodococcus opacus B4
ORGANISM Rhodococcus opacus B4
Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
Nocardiaceae; Rhodococcus.
REFERENCE 1 (bases 1 to 4367)
AUTHORS Sekine,M., Hosoyama,A., Kosugi,H., Yamada,R., Matsuo,Y.,
Tsukatani,N., Fujisawa,T., Takarada,H., Omata,S., Kishi,E.,
Shimizu,A., Koga,C., Saito,S., Nakazawa,H., Sekigawa,T.,
Nishiko,R., Takeyama,M., Tanikawa,S., Tago,S., Fujita,N., Kato,J.,
Na,K.S., Ohtake,H., Nagayasu,K. and Harayama,S.
TITLE Direct Submission
JOURNAL Submitted (30-MAR-2009) to the DDBJ/EMBL/GenBank databases.
Contact:Director-General Department of Biotechnology
National Institute of Technology and Evaluation, NITE Genome
Analysis Center (NGAC), Department of Biotechnology; 2-49-10
Nishihara, Shibuya-ku, Tokyo 151-0066, Japan
URL :http://www.bio.nite.go.jp/
REFERENCE 2
AUTHORS Takarada,H., Sekine,M., Hosoyama,A., Yamada,R., Fujisawa,T.,
Omata,S., Shimizu,A., Tsukatani,N., Tanikawa,S., Fujita,N. and
Harayama,S.
TITLE Comparison of the complete genome sequences of Rhodococcus
erythropolis PR4 and Rhodococcus opacus B4
JOURNAL Unpublished (2009)
REFERENCE 3
AUTHORS Na,K.S., Nagayasu,K., Kuroda,A., Takiguchi,N., Ikeda,T., Ohtake,H.
and Kato,J.
TITLE Development of a genetic transformation system for
benzene-tolerant Rhodococcus opacus strains.
JOURNAL J. Biosci. Bioeng. 99, 408-414 (2005)
COMMENT Kato,J., Na,K., Ohtake,H. and Nagayasu,K. are at Department of
Molecular Biotechnology, Hiroshima University.
Rhodococcus opacus B-4 strain was isolated as a benzene-tolerant
bacterium (Kyung-su Na et al 2005). The complete genome analysis
of R. opacus B-4 was carried out by NITE. The genome analysis
indicated that this strain has one linear chromosome, two linear
plasmids pROB01, pROB02, and three circular plasmids pKNR, pKNR01,
pKNR02. The nucleotide sequence of the linear plasmid pKNR01 was
previously determined independently by Kato et al and was
registered with the accession number AB193159, which is now merged
with this record with the permission of Dr. Kato.
The differences between the entry AB193159 and this record are as
follows.
'A' was deleted between positions 2211 and 2212.
'T' was deleted between positions 2329 and 2330.
'G' at position 2719 replaced 'C'.
This work was supported by New Energy and Industrial Technology
Development Organization (NEDO).
Please visit our web site.
URL:http://www.bio.nite.go.jp/
DOGAN ; Database of Genomes Analyzed at NITE
The database contains genome sequence data, physical maps and the
genomic and proteomic data of the microorganisms analyzed.
URL:http://www.bio.nite.go.jp/dogan/Top
The microbial genomic DNA clones used for the sequencing project
are available through the NBRC.
URL:http://www.nbrc.nite.go.jp/e/index.html
FEATURES Location/Qualifiers
source 1..4367
/db_xref="taxon:632772"
/mol_type="genomic DNA"
/organism="Rhodococcus opacus B4"
/plasmid="pKNR01"
/strain="B4"
CDS complement(367..648)
/codon_start=1
/locus_tag="ROP_pKNR01-00010"
/product="hypothetical protein"
/protein_id="BAH47256.1"
/transl_table=11
/translation="MKPRDGRRDDAQFDLFNHLRKWTYSAHQSFEGDGQSWLDEVTEV
AEEFHDKLLSCRLDPMPTQLIHDLAADCAVWTWETMHTGIVTSAENSSS"
CDS complement(912..1178)
/codon_start=1
/gene="repB"
/locus_tag="ROP_pKNR01-00020"
/product="replication protein RepB"
/protein_id="BAD95582.1"
/transl_table=11
/translation="MGAAERVMRRTQTAQELADKFGVSKSTIKRLVAEDRGDYIARAQ
ERRKKAVELRKQGLKYRQIADQMNISTGSVGTLIRQARIHGEMD"
CDS complement(1178..2092)
/codon_start=1
/gene="repA"
/locus_tag="ROP_pKNR01-00030"
/product="replication protein RepA"
/protein_id="BAD95583.1"
/transl_table=11
/translation="MTAAVDAWLDRWRFKYPHATDDYVRDGQWPQPRAEALTRRYIAA
RTPSIRLGLVLDVDDDQAQWAARNADLPDPTVMTINPATEHAHLWWLLESPVYTLQAA
RQGPLRLYDRVNQGLIASVVGADQGYAQQLTQNPIHPHWTTVWGPSDPYSLKGLAKAL
GDRFPAPPDYGRKTQVRTETGRNVELFDRTREWAYREIRKQNYPSITVWRQMVTDFTA
LTNFTLDANDGIGPMSRAEAEQIGKSVAHWTHRTFTAAEFSKIQARRGKVSSSAKTEA
VRKNATAPRPKLKKVDRSALFAAAEGTD"
CDS 3257..3841
/codon_start=1
/locus_tag="ROP_pKNR01-00040"
/product="hypothetical protein"
/protein_id="BAH47257.1"
/transl_table=11
/translation="MTTPRCPRCDREIESNTSAVHGRGRPRKWCSDECRKLASEERRA
AERGIVAVELRERVVEVEKVVHRPATTAASIDRVLDDDQALQKLLNTLRHRIKEGTYR
PNRDPGTAMWMLQRLTAPVADLLAQIHSSAGTTPPMDPAQAVRTVLHSPRSCRAVLEG
LERMARSGELDGGQHTATLIALDRLVARTRRQSR"
CDS 3936..4118
/codon_start=1
/locus_tag="ROP_pKNR01-00050"
/product="hypothetical protein"
/protein_id="BAH47258.1"
/transl_table=11
/translation="MMDLDAQVAVLRDQLNALGRQVREVTEAAGEQDKPDVVDALADV
RMHLNDAAAVLGDVIV"
CDS complement(join(4254..4367,1..285))
/codon_start=1
/locus_tag="ROP_pKNR01-00060"
/product="hypothetical protein"
/protein_id="BAH47259.1"
/transl_table=11
/translation="MGDAAGDRHRRVGRSGRGFEHPEHNGLGLDDPLTFGSDLFSGSK
LNMRECQFSFEFGDPTGGLGRPLHMVMNRVVNRVGRGGSVRVQSSSQQVVDPNRPALL
RFDRTGQLACLNAAEQGCPTDPCTPSRLGQ"
BASE COUNT 756 a 1404 c 1414 g 793 t
ORIGIN
1 gtcgacgacc tgctggctgc tggactgcac ccggacagac ccgccccgcc cgaccctgtt
61 cacgaccctg ttcatgacca tgtgcagcgg ccgcccgagg ccgcccgtcg gatcaccgaa
121 ctcgaacgag aactggcact cgcgcatgtt cagcttgctg ccgctgaaca ggtcgctgcc
181 gaacgtgagc gggtcatcca agcccaggcc gttgtgctcc ggatgctcga agccgcgccc
241 agaccggccg actcgccgat gccggtcgcc ggcggcgtcg cccacaccaa cccaccgccg
301 gctgaagaac cgccggccac cctttcaccc gatcggcggg cggaaccggg caatcccctg
361 acctggctac gaagacgaat tctcggctga ggtcacgatt cccgtgtgca tcgtttccca
421 cgtccacact gcgcagtcgg ccgcgaggtc atggatcagt tgggtaggca tcgggtcgag
481 gcggcacgaa agaagcttgt cgtggaattc ctccgccacc tcggtgacct catcgagcca
541 gctctgtccg tcgccctcga agctttgatg cgcggagtac gtccactttc tcaggtggtt
601 gaacagatca aactgcgcgt cgtcccggcg gccgtcacgt ggtttcaccg gccgatgctt
661 acgaggttcc ggtgttgcgg caggtggttt cggtttcgcg ggtggtgtct tccggggtgg
721 aggtgtccgg gcgggggcat gccaccggca cggccccttg cctccggttg cgataatgca
781 ttgtgtccca tcatctttga gcgccccgca ttggcgttcc ggagcatgtg tcgggcacac
841 atcgcggcct gccaaggctg gatgaccgca taggtcacca gttctggtct gtccgatgca
901 ttgcaacggt ttcagtccat ctcgccgtgt atgcgggcct gacggatcaa cgttcccacc
961 gagccagtcg agatgttcat ctggtcggcg atctgccggt acttcaaacc ttgtttgcgc
1021 agttccacag ccttcttgcg gcgttcctgc gcacgagcga tgtagtcgcc tcggtcttcg
1081 gcgacgagcc gtttgatggt gcttttcgag acgccgaact tgtcagccaa ctcctgcgcg
1141 gtctgcgtgc gacgcatcac gcgttctgca gcacccatca gtccgtcccc tctgctgctg
1201 cgaacagtgc cgatcgatcg accttcttga gcttcggccg cggcgcggtg gcgttcttcc
1261 gtaccgcttc cgtttttgcg ctgctgctca ctttgccgcg gcgtgcctgg attttcgaga
1321 actcggcggc ggtgaaggtg cggtgggtcc agtgggcgac tgatttgccg atctgctcgg
1381 cctcggcccg actcatgggg ccgatcccgt cgttggcgtc gagggtgaag ttggtcaggg
1441 cggtgaagtc ggtgaccatc tgccgccaca cagtgatcga cgggtagttc tgtttccgga
1501 tctcgcggta ggcccattcc cgggtgcggt cgaacagttc gacgttccgg cccgtttcgg
1561 tcctgacctg tgtcttgcgg ccgtagtccg gtggggcggg gaaacggtca ccgagcgctt
1621 ttgcgaggcc tttgagcgag tacggatccg agggacccca gaccgtcgtc cagtgcgggt
1681 ggatcgggtt ctgggtgagc tgctgcgcgt agccctgatc ggcgccgacc accgaggcga
1741 tcagcccctg gttcacccgg tcgtagagcc gcagcgggcc ctgtcgggct gcctggaggg
1801 tgtagaccgg gctttcgagc agccaccaca ggtgcgcgtg ctcggtcgcg ggattgatcg
1861 tcatcacggt cggatcgggc agatccgcgt tacgtgcggc ccactgcgcc tggtcgtcgt
1921 ccacgtcgag caccaagccc aacctgatcg acggggtgcg ggccgcaatg tagcggcggg
1981 tgagcgcctc cgcgcgcggc tgcggccact gcccgtcccg gacgtagtca tccgtcgcgt
2041 gcgggtattt gaaccgccag cggtccaacc aggcgtcaac agcagcggtc atgaccgcca
2101 agctagggcc ggatctgtac cgatcggggg aggcgcgccg caaattattt aagagtctcg
2161 ctagcaaacc atgtcaggtg ttgcggtggg ttccgggtaa acctccaccc gaattattta
2221 agagtctcgc tagctaagcc ctatctgatg ctgcgcgggg ggtccttcgc actgaatctc
2281 aaaggtggcc ggctgaattt cgtcgcgcga aaacctccct ggacagttct ggaattcagc
2341 aagaggtgtg tctgaacttc ggtgtttttt tggggggtga ctccagcggg gtgggcacaa
2401 cgcgaacaga gaccttgtgt gtacgacggc gggaggtaag tcgggtacgg ctcggactgc
2461 ggtagagcaa ccgtcgaatc gatttcgagc agagcgagca gagcaagata ttccaaaact
2521 ccggggttcc tcggcggcct cccccgtctg tttgctcaac cgagggagac ctggcggtcc
2581 cgcgtttccg gacgcgcggg accgcctacc gctcgagcac cccgagagcg acggaggctc
2641 ccgggacccc gcccgtgcgg agagcaagat aggcacttac ctgacgtaag tgaggagtga
2701 cggtgcgcga gcggtcgcgc accgttggtg ttgtttcggg cggagcaaga tggagactta
2761 ggtgccctaa gtcgtcggag cccggttcgg ggcgctgcgc tgggccggtg gaacctggtg
2821 agcgcttaca gcgtcgggca gatgcgcgaa ctgtccgatc acctcccaca ggcaccggtg
2881 cagatctgcg gctgcccggg actcgggtgc ggtaacagtg tccgattccg gagaccacga
2941 gatctgtggg tcgatgtgag agacggcgcg gctcaatccg cgcgcccatt cccgcagacc
3001 ggtgtcatcg acgaacgggt cccaggcgcg gatccacgcc gacaacgccg gccaccgcag
3061 ttcgtcactc accaccgaac acctcaacac gtctccgact ttgctagaga tccgatctgt
3121 tgccgtcatc cgcgagtagt gaaagttccg agcaagcctc gttcgaacag gcacaaccag
3181 ccatacaccg acacacctct tgcactcagg agtgaataga gggctatttt cgcgcgacga
3241 aattaggatc gcggctatga cgacaccgcg atgcccccgc tgtgaccgcg agatcgagag
3301 caacaccagc gccgtgcacg gtcgcggccg gccgcgaaag tggtgctcgg acgagtgccg
3361 caaactcgcc tcggaggaac gccgcgcggc cgaacgcggc atcgtcgcgg tggagttgcg
3421 cgagcgggtg gtcgaggtcg agaaggtggt gcaccggccg gccacgacgg ctgcatccat
3481 cgaccgcgta ctcgacgacg accaggcgct gcagaaactg ttgaacacgc tgcggcaccg
3541 catcaaggaa ggcacctacc gacccaaccg tgaccctggg actgcaatgt ggatgctgca
3601 gagactcacg gctcccgtgg ccgatctgct cgcccagatc cactccagcg ccggcaccac
3661 cccgcccatg gatccggcgc aggccgtgcg aacggtgctc cactcgccac gctcatgccg
3721 cgcggtcctg gaaggcctcg aacggatggc ccgctccggt gagctcgacg gcggccagca
3781 caccgccact ctgatagctc tcgacaggct cgtagcccgc acccggcggc agagccggta
3841 gaaaacgggc tgccgcggca ccgctgcgcg ctgccttgcc accgaaaaca agttcgccga
3901 catgcccgca agcttcagac cgaaggagtg atcgcatgat ggatctagac gcacaggtag
3961 ccgtattgcg cgatcagttg aacgccctcg gccgccaggt ccgcgaagtc acagaggccg
4021 ccggcgagca ggacaagccc gacgtcgtcg acgccctggc cgacgtgcgc atgcacctca
4081 acgacgccgc cgcagtcctc ggggacgtga tcgtatgacc gcacaggtaa ccgaagatcg
4141 acacctgtgc gcgtggtgtg ggcagcacat gaacatgtgc gcccacactg atggccatgc
4201 ccccggacac ccctgaccac gacgcccgcc tgaacagcca acggccccgg tggtcactgt
4261 ccgaggcggc taggcgtaca ggggtcagtc gggcaaccct gctccgccgc attgaggcag
4321 gcaagctgcc cggtgcggtc aaaacggagg agagctggtc tattggg
//