LOCUS USG64314.1 421 aa PRT BCT 16-JUN-2022 DEFINITION Brevibacillus sp. BB3-R1 group II intron reverse transcriptase/ maturase protein. ACCESSION CP098755-3765 PROTEIN_ID USG64314.1 SOURCE Brevibacillus sp. BB3-R1 ORGANISM Brevibacillus sp. BB3-R1 Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus. REFERENCE 1 (bases 1 to 5627247) AUTHORS Heo,J., Lee,D., Won,M., Han,B.-H., Hong,S.-B. and Kwon,S.-W. TITLE Genome sequencing of Brevibacillus sp. BB3-R1 JOURNAL Unpublished REFERENCE 2 (bases 1 to 5627247) AUTHORS Heo,J., Lee,D., Won,M., Han,B.-H., Hong,S.-B. and Kwon,S.-W. TITLE Direct Submission JOURNAL Submitted (09-JUN-2022) Agricultural Mircrobiology Division, National Institute of Agricultural Sciences, 166 Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, South Korea COMMENT Bacteria and source DNA available from KACC. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: MAY-2022 Assembly Method :: Microbial Assembly Application of SMRTLink v. 10.1 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 811.0x Sequencing Technology :: PacBio Sequel; Illumina HiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 06/10/2022 13:07:44 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.1 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,522 CDSs (total) :: 5,372 Genes (coding) :: 5,285 CDSs (with protein) :: 5,285 Genes (RNA) :: 150 rRNAs :: 9, 10, 10 (5S, 16S, 23S) complete rRNAs :: 9, 10, 10 (5S, 16S, 23S) tRNAs :: 116 ncRNAs :: 5 Pseudo Genes (total) :: 87 CDSs (without protein) :: 87 Pseudo Genes (ambiguous residues) :: 0 of 87 Pseudo Genes (frameshifted) :: 28 of 87 Pseudo Genes (incomplete) :: 62 of 87 Pseudo Genes (internal stop) :: 15 of 87 Pseudo Genes (multiple problems) :: 18 of 87 CRISPR Arrays :: 2 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Brevibacillus sp. BB3-R1" /mol_type="genomic DNA" /strain="BB3-R1" /isolation_source="Cow fecal" /culture_collection="KACC:22663" /db_xref="taxon:2950604" /country="South Korea: Yuseong-gu, Daejeon-si" /collection_date="2022-03-23" /collected_by="Jun Heo, Daseul Lee" /identified_by="Jun Heo" protein /gene="ltrA" /locus_tag="NDK47_19445" /EC_number="2.7.7.49" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_012684507.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology. GO_function: GO:0003964 - RNA-directed DNA polymerase activity [Evidence IEA]; GO_process: GO:0000373 - Group II intron splicing [Evidence IEA]" /transl_table=11 BEGIN 1 MDLLEKVLSR ENLRMALQRV EANKGVGGVD GVSTEQLRDY LHEHWQTIRE ELERGTYRPS 61 PVRRVEIPKP DGGVRLLGIP TVVDRFIQQA ILQVLTPIFD PTFSESSYGF RPKRSAHMAV 121 RKAQAYIREG YRFVVDIDLE KFFDRVNHDI LMSRVARKIQ DKRLLKLIRS YLNAGVMIKG 181 ICTTSSEGTP QGGPLSPLLA NILLDDLDKE LEKRGHRFCR YADDCNVYVK TRRAGERVKK 241 SMKDYLEKVL RLKVNEEKSA VDRPWKRKFL GFSFTFVKQT TVRIHPKSLL KLKEKIRTIT 301 NPVWSISLEE RVERLNQYLM GWIGYFALAD AKGILQSIEE WTRRRLRLCL WTQWKRVRTR 361 YRELRSLGIS HTKAIEIANT RKGAWRTTKT PHIHKALGIA YWQQQGLKSL TQRYFDIRQA 421 W //