LOCUS UQS85597.1 751 aa PRT BCT 19-SEP-2022 DEFINITION Apilactobacillus apisilvae DNA helicase PcrA protein. ACCESSION CP093362-611 PROTEIN_ID UQS85597.1 SOURCE Apilactobacillus apisilvae ORGANISM Apilactobacillus apisilvae Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Apilactobacillus. REFERENCE 1 (bases 1 to 1469670) AUTHORS Oliphant,S.A., Watson-Haigh,N.S., Sumby,K.M., Gardner,J., Groom,S. and Jiranek,V. TITLE Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov. sp. nov., Bombilactobacillus folatiphilus sp. nov. and Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid bacterial isolates from stingless bees Tetragonula carbonaria and Austroplebeia australis JOURNAL Int J Syst Evol Microbiol 72 (9) (2022) PUBMED 36094463 REFERENCE 2 (bases 1 to 1469670) AUTHORS Oliphant,S.A., Sumby,K.M., Gardner,J.M., Watson-Haigh,N.S. and Jiranek,V. TITLE Direct Submission JOURNAL Submitted (11-MAR-2022) Wine Science, The University of Adelaide, PMB 1, Glen Osmond, South Australia 5064, Australia COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: NOV-2020 Assembly Method :: Smrtlink v. 9.0 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 11482x Sequencing Technology :: PacBio Sequel II ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 03/14/2022 11:52:50 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.0 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 1,575 CDSs (total) :: 1,494 Genes (coding) :: 1,480 CDSs (with protein) :: 1,480 Genes (RNA) :: 81 rRNAs :: 5, 5, 5 (5S, 16S, 23S) complete rRNAs :: 5, 5, 5 (5S, 16S, 23S) tRNAs :: 63 ncRNAs :: 3 Pseudo Genes (total) :: 14 CDSs (without protein) :: 14 Pseudo Genes (ambiguous residues) :: 0 of 14 Pseudo Genes (frameshifted) :: 5 of 14 Pseudo Genes (incomplete) :: 11 of 14 Pseudo Genes (internal stop) :: 1 of 14 Pseudo Genes (multiple problems) :: 3 of 14 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Apilactobacillus apisilvae" /mol_type="genomic DNA" /strain="SG5_A10" /isolation_source="Bee" /host="Austroplebeia australis" /type_material="type strain of Apilactobacillus apisilvae" /db_xref="taxon:2923364" /country="Australia: Brisbane" /lat_lon="27.4810 S 153.0121 E" /collection_date="2020-04-30" protein /gene="pcrA" /locus_tag="MOO46_03305" /EC_number="3.6.4.12" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_011673639.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology. GO_function: GO:0003678 - DNA helicase activity [Evidence IEA]; GO_process: GO:0006268 - DNA unwinding involved in DNA replication [Evidence IEA]; GO_process: GO:0006281 - DNA repair [Evidence IEA]" /transl_table=11 BEGIN 1 MSVNDVISGM NGKQKEAVLC TEGPLLIMAG AGSGKTRVLT HRIAYLIEEK QVLPWNILAI 61 TFTNKASKEM KERVSNLLDY GGNEVWISTF HALCVRILRS HIDKIGYNRA FTIAGSSEQR 121 TLVKRILKDL NIDPKKNDPR AILSAISNAK NDLKTPYEYQ ESLNHTSPFE ETVGKVYTEY 181 QKRLHNNQAL DFDDLIMMTI QLFEDVPDVL DYYQNKFHYI HVDEYQDTNE AQYKLVTMLA 241 KKYKNICVVG DADQSIYGWR GANMNNILNF QNDYKNANTV MLEQNYRSTK NILNAANDVI 301 KNNVSRKEKN LWTENKDGDK VSYYRGQSEV DEAHFVVTQI KKIIEEKNYS YNDFAILYRT 361 NAQSRIIEET FLKSNIPYMI VGGHKFYDRK EIQDVLAYLT LIANPSDSMS LERIINEPKR 421 GIGASSIEKL RVFAQENNWS MLEATKNIEL NTTLSNRART SIDQFGKMMT GLQKIAQENK 481 VTDVTEHILD DSGYLDNLKK SKSLESKARI ENIEEFISVT QQFDNNRTED EGDEPLTDFL 541 ADLALVSAQD DVDEQTPQVT LMTLHAAKGL EFPVVFLMGM EEGIFPLGRS LENEDELEEE 601 RRLAYVGITR AQKKLFITNA FSRMLYGRRQ TNPESRFIDE ISDNLIHSEN NFSKNNSLKT 661 PFDSSRGRRM QQFSTQRAYR QPSQKVQKPK GSGADKKSWI VGDKVQHKAW GIGTVVKING 721 SGEDTELDIA FPDQGLKRLL AAFAPINKVE K //