LOCUS       UQS85548.1               460 aa    PRT              BCT 19-SEP-2022
DEFINITION  Apilactobacillus apisilvae UDP-N-acetylmuramoyl-L-alanine-
            -D-glutamate ligase protein.
ACCESSION   CP093362-557
PROTEIN_ID  UQS85548.1
SOURCE      Apilactobacillus apisilvae
  ORGANISM  Apilactobacillus apisilvae
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
            Apilactobacillus.
REFERENCE   1  (bases 1 to 1469670)
  AUTHORS   Oliphant,S.A., Watson-Haigh,N.S., Sumby,K.M., Gardner,J., Groom,S.
            and Jiranek,V.
  TITLE     Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov.
            sp. nov., Bombilactobacillus folatiphilus sp. nov. and
            Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid
            bacterial isolates from stingless bees Tetragonula carbonaria and
            Austroplebeia australis
  JOURNAL   Int J Syst Evol Microbiol 72 (9) (2022)
   PUBMED   36094463
REFERENCE   2  (bases 1 to 1469670)
  AUTHORS   Oliphant,S.A., Sumby,K.M., Gardner,J.M., Watson-Haigh,N.S. and
            Jiranek,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2022) Wine Science, The University of Adelaide,
            PMB 1, Glen Osmond, South Australia 5064, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2020
            Assembly Method        :: Smrtlink v. 9.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 11482x
            Sequencing Technology  :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/14/2022 11:52:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.0
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,494
            Genes (coding)                    :: 1,480
            CDSs (with protein)               :: 1,480
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Apilactobacillus apisilvae"
                     /mol_type="genomic DNA"
                     /strain="SG5_A10"
                     /isolation_source="Bee"
                     /host="Austroplebeia australis"
                     /type_material="type strain of Apilactobacillus apisilvae"
                     /db_xref="taxon:2923364"
                     /country="Australia: Brisbane"
                     /lat_lon="27.4810 S 153.0121 E"
                     /collection_date="2020-04-30"
     protein         /gene="murD"
                     /locus_tag="MOO46_02900"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010620116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008764 - UDP-N-acetylmuramoylalanine-D-glutamate
                     ligase activity [Evidence IEA]; GO_process: GO:0009252 -
                     peptidoglycan biosynthetic process [Evidence IEA]"
                     /transl_table=11
BEGIN
        1 MKNINKYTNK NILVLGAGKS GTNAAKLLIK LGAKVVLSDS KTEEQITDKA LLESIGVKVL
       61 AGKQSPDILA SNDFDLVVKN PGIPYDTPIV KKAQELKLPI IVEVELASEI TDAELIGVTG
      121 SNGKTTTTTM INKMLNMERT SGESRVAGNI GVPASNVAEK SNSDDDIVME LSSFMLLGIT
      181 ELHPHIAVLT NIFSNHLDYH KTRQNYVNAK MRITMNQNKN DFFIVNFDNE EWRSLSKKSN
      241 AQVIPFSRKG NSTKGAYEKD GKLYFKDEYI MDAQNIRIPG EQNIENALAA IAVAKIKQRS
      301 NVAIKHVLET FGGVRHRVQY VLESEDRKFY NDSKATDIEA TQMALRGFNK PVVLLAGGLD
      361 RGYKFDQLVP DLKKHVSGIV LFGETRYLLE DAAKQANIKN IKLVNNLDEA VPLAYKLSNQ
      421 GDIILLSPAN ASWDQFSTFE VRGDQFIKDV ENLTGKKEEK
//