LOCUS UQS85548.1 460 aa PRT BCT 19-SEP-2022 DEFINITION Apilactobacillus apisilvae UDP-N-acetylmuramoyl-L-alanine- -D-glutamate ligase protein. ACCESSION CP093362-557 PROTEIN_ID UQS85548.1 SOURCE Apilactobacillus apisilvae ORGANISM Apilactobacillus apisilvae Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Apilactobacillus. REFERENCE 1 (bases 1 to 1469670) AUTHORS Oliphant,S.A., Watson-Haigh,N.S., Sumby,K.M., Gardner,J., Groom,S. and Jiranek,V. TITLE Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov. sp. nov., Bombilactobacillus folatiphilus sp. nov. and Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid bacterial isolates from stingless bees Tetragonula carbonaria and Austroplebeia australis JOURNAL Int J Syst Evol Microbiol 72 (9) (2022) PUBMED 36094463 REFERENCE 2 (bases 1 to 1469670) AUTHORS Oliphant,S.A., Sumby,K.M., Gardner,J.M., Watson-Haigh,N.S. and Jiranek,V. TITLE Direct Submission JOURNAL Submitted (11-MAR-2022) Wine Science, The University of Adelaide, PMB 1, Glen Osmond, South Australia 5064, Australia COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: NOV-2020 Assembly Method :: Smrtlink v. 9.0 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 11482x Sequencing Technology :: PacBio Sequel II ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 03/14/2022 11:52:50 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.0 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 1,575 CDSs (total) :: 1,494 Genes (coding) :: 1,480 CDSs (with protein) :: 1,480 Genes (RNA) :: 81 rRNAs :: 5, 5, 5 (5S, 16S, 23S) complete rRNAs :: 5, 5, 5 (5S, 16S, 23S) tRNAs :: 63 ncRNAs :: 3 Pseudo Genes (total) :: 14 CDSs (without protein) :: 14 Pseudo Genes (ambiguous residues) :: 0 of 14 Pseudo Genes (frameshifted) :: 5 of 14 Pseudo Genes (incomplete) :: 11 of 14 Pseudo Genes (internal stop) :: 1 of 14 Pseudo Genes (multiple problems) :: 3 of 14 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Apilactobacillus apisilvae" /mol_type="genomic DNA" /strain="SG5_A10" /isolation_source="Bee" /host="Austroplebeia australis" /type_material="type strain of Apilactobacillus apisilvae" /db_xref="taxon:2923364" /country="Australia: Brisbane" /lat_lon="27.4810 S 153.0121 E" /collection_date="2020-04-30" protein /gene="murD" /locus_tag="MOO46_02900" /EC_number="6.3.2.9" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_010620116.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology. GO_function: GO:0008764 - UDP-N-acetylmuramoylalanine-D-glutamate ligase activity [Evidence IEA]; GO_process: GO:0009252 - peptidoglycan biosynthetic process [Evidence IEA]" /transl_table=11 BEGIN 1 MKNINKYTNK NILVLGAGKS GTNAAKLLIK LGAKVVLSDS KTEEQITDKA LLESIGVKVL 61 AGKQSPDILA SNDFDLVVKN PGIPYDTPIV KKAQELKLPI IVEVELASEI TDAELIGVTG 121 SNGKTTTTTM INKMLNMERT SGESRVAGNI GVPASNVAEK SNSDDDIVME LSSFMLLGIT 181 ELHPHIAVLT NIFSNHLDYH KTRQNYVNAK MRITMNQNKN DFFIVNFDNE EWRSLSKKSN 241 AQVIPFSRKG NSTKGAYEKD GKLYFKDEYI MDAQNIRIPG EQNIENALAA IAVAKIKQRS 301 NVAIKHVLET FGGVRHRVQY VLESEDRKFY NDSKATDIEA TQMALRGFNK PVVLLAGGLD 361 RGYKFDQLVP DLKKHVSGIV LFGETRYLLE DAAKQANIKN IKLVNNLDEA VPLAYKLSNQ 421 GDIILLSPAN ASWDQFSTFE VRGDQFIKDV ENLTGKKEEK //