LOCUS UQS85292.1 803 aa PRT BCT 19-SEP-2022 DEFINITION Apilactobacillus apisilvae phenylalanine--tRNA ligase subunit beta protein. ACCESSION CP093362-280 PROTEIN_ID UQS85292.1 SOURCE Apilactobacillus apisilvae ORGANISM Apilactobacillus apisilvae Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Apilactobacillus. REFERENCE 1 (bases 1 to 1469670) AUTHORS Oliphant,S.A., Watson-Haigh,N.S., Sumby,K.M., Gardner,J., Groom,S. and Jiranek,V. TITLE Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov. sp. nov., Bombilactobacillus folatiphilus sp. nov. and Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid bacterial isolates from stingless bees Tetragonula carbonaria and Austroplebeia australis JOURNAL Int J Syst Evol Microbiol 72 (9) (2022) PUBMED 36094463 REFERENCE 2 (bases 1 to 1469670) AUTHORS Oliphant,S.A., Sumby,K.M., Gardner,J.M., Watson-Haigh,N.S. and Jiranek,V. TITLE Direct Submission JOURNAL Submitted (11-MAR-2022) Wine Science, The University of Adelaide, PMB 1, Glen Osmond, South Australia 5064, Australia COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: NOV-2020 Assembly Method :: Smrtlink v. 9.0 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 11482x Sequencing Technology :: PacBio Sequel II ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 03/14/2022 11:52:50 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.0 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 1,575 CDSs (total) :: 1,494 Genes (coding) :: 1,480 CDSs (with protein) :: 1,480 Genes (RNA) :: 81 rRNAs :: 5, 5, 5 (5S, 16S, 23S) complete rRNAs :: 5, 5, 5 (5S, 16S, 23S) tRNAs :: 63 ncRNAs :: 3 Pseudo Genes (total) :: 14 CDSs (without protein) :: 14 Pseudo Genes (ambiguous residues) :: 0 of 14 Pseudo Genes (frameshifted) :: 5 of 14 Pseudo Genes (incomplete) :: 11 of 14 Pseudo Genes (internal stop) :: 1 of 14 Pseudo Genes (multiple problems) :: 3 of 14 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Apilactobacillus apisilvae" /mol_type="genomic DNA" /strain="SG5_A10" /isolation_source="Bee" /host="Austroplebeia australis" /type_material="type strain of Apilactobacillus apisilvae" /db_xref="taxon:2923364" /country="Australia: Brisbane" /lat_lon="27.4810 S 153.0121 E" /collection_date="2020-04-30" protein /gene="pheT" /locus_tag="MOO46_01500" /EC_number="6.1.1.20" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019252476.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology. GO_component: GO:0009328 - phenylalanine-tRNA ligase complex [Evidence IEA]; GO_function: GO:0004826 - phenylalanine-tRNA ligase activity [Evidence IEA]; GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation [Evidence IEA]" /transl_table=11 BEGIN 1 MKVSYKWLKE YIDLDIPAKD LAEKIERSSV EVDSVDKPSE GLKKIVVGQI LSLEDHPDSD 61 HLHVCQIDVG EDEPIQIVCG APNVEKGKKV IVALSGARIA DNIKIKKSKM RGIQSNGMLC 121 ALDEIGFPKD VVPKEWADGI YFLPDDAKNG DDVYSYIGMD DELIDLDVTP NRGDMLSIYG 181 TLHDLSAIYN KHVNYDDVTV KETSGKKTSD QVNSDVDENI APIYKNRIVN NVNIKSSPLW 241 LQIRLWNAGI KPINNIIDIT NYVMLKFGQP MHAYDLDKLN GNKVTVRNAK KGEKITTLGE 301 DNLNLDEKDI IIADDRPIAL AGVIGGLDTS ITKETKNVLF ECAVFDPIKV RKMARKHVIH 361 TEASQRFERG VNHSGLDEAM ENACQMSNEL ANGDVCDDIL DGTEYKISPV VVEITLTKIN 421 HILGTHLSYE QVQDIFERLG FIVHRNDDSF SVTVPVRRGD IHIDADLIEE VARIYGYDNL 481 PSTLPTTTMT TGKLQPKQQL MRDSRHILEG LGLTHAISYA LTTSEKANMF MMQNSFETNL 541 SYPMTNDHET LRMNLLTGLL DDIAYNKARN VSDVALYEQG RVFYKDTKDQ VKPSEIEHIA 601 GAISGSLISS NWDENNKEVD FYQIKGIVNK YLETIGFNGQ IDYVSNAEYP EMHPGRTADI 661 MIHGHYVGFV GEVHPNIAKQ FKINDTYVFD LNLDSLNDLP KSDQSYESIS KYPSISRDIA 721 LLVDDNITNS DIVKVIEKRG GAFLKEVNIF DVYNGEHVPE GKKSMAYNLV FQNKRDTLVD 781 DDVNKVMDKI QRNLEKEFDV EVR //