LOCUS UQS85013.1 455 aa PRT BCT 19-SEP-2022 DEFINITION Apilactobacillus apisilvae tRNA lysidine(34) synthetase TilS protein. ACCESSION CP093362-1366 PROTEIN_ID UQS85013.1 SOURCE Apilactobacillus apisilvae ORGANISM Apilactobacillus apisilvae Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Apilactobacillus. REFERENCE 1 (bases 1 to 1469670) AUTHORS Oliphant,S.A., Watson-Haigh,N.S., Sumby,K.M., Gardner,J., Groom,S. and Jiranek,V. TITLE Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov. sp. nov., Bombilactobacillus folatiphilus sp. nov. and Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid bacterial isolates from stingless bees Tetragonula carbonaria and Austroplebeia australis JOURNAL Int J Syst Evol Microbiol 72 (9) (2022) PUBMED 36094463 REFERENCE 2 (bases 1 to 1469670) AUTHORS Oliphant,S.A., Sumby,K.M., Gardner,J.M., Watson-Haigh,N.S. and Jiranek,V. TITLE Direct Submission JOURNAL Submitted (11-MAR-2022) Wine Science, The University of Adelaide, PMB 1, Glen Osmond, South Australia 5064, Australia COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: NOV-2020 Assembly Method :: Smrtlink v. 9.0 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 11482x Sequencing Technology :: PacBio Sequel II ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 03/14/2022 11:52:50 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.0 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 1,575 CDSs (total) :: 1,494 Genes (coding) :: 1,480 CDSs (with protein) :: 1,480 Genes (RNA) :: 81 rRNAs :: 5, 5, 5 (5S, 16S, 23S) complete rRNAs :: 5, 5, 5 (5S, 16S, 23S) tRNAs :: 63 ncRNAs :: 3 Pseudo Genes (total) :: 14 CDSs (without protein) :: 14 Pseudo Genes (ambiguous residues) :: 0 of 14 Pseudo Genes (frameshifted) :: 5 of 14 Pseudo Genes (incomplete) :: 11 of 14 Pseudo Genes (internal stop) :: 1 of 14 Pseudo Genes (multiple problems) :: 3 of 14 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Apilactobacillus apisilvae" /mol_type="genomic DNA" /strain="SG5_A10" /isolation_source="Bee" /host="Austroplebeia australis" /type_material="type strain of Apilactobacillus apisilvae" /db_xref="taxon:2923364" /country="Australia: Brisbane" /lat_lon="27.4810 S 153.0121 E" /collection_date="2020-04-30" protein /gene="tilS" /locus_tag="MOO46_07180" /EC_number="6.3.4.19" /inference="COORDINATES: protein motif:HMM:TIGR02432.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology. GO_function: GO:0016879 - ligase activity, forming carbon-nitrogen bonds [Evidence IEA]; GO_process: GO:0006400 - tRNA modification [Evidence IEA]" /transl_table=11 BEGIN 1 MQDGETLSTQ FFNLITNNEW FSPLDKVVIG VSAGVDSMVL LYLMTHLPDN VRPKIIVAHI 61 NHKLRLQSEK EQSYIESFCK NNDLQCEIGI WEQKNHPTNG IENFARQYRY SFFKKIMTKY 121 SANYLLTAHH LNDQSETILM KLIRGSYVKQ ISGIQRIRKF NNGFLLRPLL DFKKSELKQY 181 AIEKHIKWFE DSTNQELDVQ RNRVRHQIIP MMEKENPQVI KHLGNFSKQL NQIIDTNNDL 241 IFRLLDQLKL PDGGYNLELF NGYSKNVKFS ILETILNPIN NPALANNNNM YNIIEILSAD 301 SKPQLKIDLS NEYQIYKSYN KFGLLNKNNN VNLLKSDNFV VPLNQWFTTL DGKSVLFTDK 361 EIKSVDKYSR VSIFSLSADD FPLSVRLTDK KDKITLKSGG HQFAKRIFIN KKVPNDLRKT 421 AQTLLTAKGK VLSILGYSES TILNHSNKRY NLIIK //