LOCUS UQS82890.1 1387 aa PRT BCT 19-SEP-2022
DEFINITION Bombilactobacillus folatiphilus type II CRISPR RNA-guided
endonuclease Cas9 protein.
ACCESSION CP093366-751
PROTEIN_ID UQS82890.1
SOURCE Bombilactobacillus folatiphilus
ORGANISM Bombilactobacillus folatiphilus
Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
Bombilactobacillus.
REFERENCE 1 (bases 1 to 1622785)
AUTHORS Oliphant,S.A., Watson-Haigh,N.S., Sumby,K.M., Gardner,J., Groom,S.
and Jiranek,V.
TITLE Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov.
sp. nov., Bombilactobacillus folatiphilus sp. nov. and
Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid
bacterial isolates from stingless bees Tetragonula carbonaria and
Austroplebeia australis
JOURNAL Int J Syst Evol Microbiol 72 (9) (2022)
PUBMED 36094463
REFERENCE 2 (bases 1 to 1622785)
AUTHORS Oliphant,S.A., Sumby,K.M., Gardner,J.M., Watson-Haigh,N.S. and
Jiranek,V.
TITLE Direct Submission
JOURNAL Submitted (11-MAR-2022) Wine Science, The University of Adelaide,
PMB 1, Glen Osmond, South Australia 5064, Australia
COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (PGAP). Information about PGAP can be found here:
https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
##Genome-Assembly-Data-START##
Assembly Date :: NOV-2020
Assembly Method :: Smrtlink v. 9.0
Genome Representation :: Full
Expected Final Version :: Yes
Genome Coverage :: 7404x
Sequencing Technology :: PacBio Sequel II
##Genome-Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Date :: 03/14/2022 11:45:56
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline (PGAP)
Annotation Method :: Best-placed reference protein
set; GeneMarkS-2+
Annotation Software revision :: 6.0
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes (total) :: 1,623
CDSs (total) :: 1,550
Genes (coding) :: 1,527
CDSs (with protein) :: 1,527
Genes (RNA) :: 73
rRNAs :: 4, 4, 4 (5S, 16S, 23S)
complete rRNAs :: 4, 4, 4 (5S, 16S, 23S)
tRNAs :: 58
ncRNAs :: 3
Pseudo Genes (total) :: 23
CDSs (without protein) :: 23
Pseudo Genes (ambiguous residues) :: 0 of 23
Pseudo Genes (frameshifted) :: 12 of 23
Pseudo Genes (incomplete) :: 9 of 23
Pseudo Genes (internal stop) :: 10 of 23
Pseudo Genes (multiple problems) :: 7 of 23
CRISPR Arrays :: 3
##Genome-Annotation-Data-END##
FEATURES Qualifiers
source /organism="Bombilactobacillus folatiphilus"
/mol_type="genomic DNA"
/strain="SG4_D2"
/isolation_source="Bee"
/host="Tetragonula carbonaria"
/type_material="type strain of Bombilactobacillus
folatiphilus"
/db_xref="taxon:2923362"
/country="Australia: Brisbane"
/lat_lon="27.4810 S 153.0121 E"
/collection_date="2020-03-20"
protein /gene="cas9"
/locus_tag="MOO45_03980"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_009557865.1"
/note="Cas9, originally named Csn1, is the large,
multifunctional signature protein of type II CRISPR/Cas
systems. It is well known even to general audiences
because its RNA-guided endonuclease activity has made it a
popular tool for custom editing of eukaryotic genomes;
Derived by automated computational analysis using gene
prediction method: Protein Homology. GO_component:
GO:0005575 - cellular_component [Evidence IEA];
GO_function: GO:0004520 - endodeoxyribonuclease activity
[Evidence IEA]; GO_process: GO:0043571 - maintenance of
CRISPR repeat elements [Evidence IEA]"
/transl_table=11
BEGIN
1 MVCLDIGTNS CGFAAMDMKN QLLHLQGKTA IGARLFEEGK SAAERRGFRT TRRRLKRRKW
61 RLRLLEEFFD DEMSQVDPYF FARMRESGLS PLDHQKTAQA IVFPTPNEDH AFYCDYPTIY
121 HLRKALMTQD KKFDLRLVYL AIHHIVKYRG NFLQKDGVDN FNASKIEVGK VLKKLNYFFA
181 EINPDHPIQL AIQNSAEIEA VLRDVKKSKT DKVKSIGELL VSDSNHDKNT KAIASQIAKA
241 IMGYKTQFET ILSQEIDSDS KSEWQFKLSD SDADDKLAAI TDQVDETGQE IIEVIQSLFG
301 AITLSGIVDE GKSLSESMVR KYDDHKKDLK LLKQVIKQHP DRDKAQNLQL AYDLYVNNRH
361 GQLLKAKNKF SAKKVMSKEE FYKTIEKNLD DSSEVNAILE KIALDTFMPK QRTSANGVIP
421 FQLHQIELDQ IIKNQSKYYP FLAQRNPIIE HQKQAAYKLD ELIRFRVPYY VGPMITKENQ
481 IKTSGTEFAW MIRNQNDPKP NEAITPWNFD EKVDRMATAN QFIKRMTTKD TYLLGEDVLP
541 ANSLLYQKFT VLNELNNLRV NGQHLKAATK QDVYENLFKQ NKTVSKKCLN AYLCQSYQMA
601 SVKIEGLADE HKFNSSLKTY NQFKKFIPLS ILDNADYQAD LEKIIEWSTI FEDRHIYQAK
661 LEQAQTEQIS WLTGKQIGCL LKLRHQGWGS LSYKLLINLH DDNGQNIIER LWDSQLNFMQ
721 IVKEPAFKSV IDQANSSLVK DNQENAVEDV LADAYTSPAN KKAIRQVVKV VADIVKAAGG
781 KIPAKFAIEF TREPQKNPQL SKQRGKQLKE AYKEIANQLV EQGVKDELDS AIQSKQLVRD
841 KYYLYFMQGG RDAYTGQTIN IDDITTKYQI DHILPQSFIK DDSLNNRVLT ASALNNAKSD
901 DVPFKHFANK LVPDLKISVS EMWKQWQKAG MISKFKLNNL QLDPDNLDKY KRAGFVNRQL
961 VETSQVIKLV TIILQTKYPE AEIITVKASY NHALRKRLDL YKSREVNDYH HAIDAYLSAI
1021 CGNYLYQMYP NLRQFFVYGK FKKMNADSDR NHAAIKELNN FNFIGLLLQK DRPGHSTVEK
1081 IYRPHTDELL FEKHPDIFDP LRHAYSFKHM LISRETYTQD QEMFGMTLYP RLERDTKKTR
1141 TLVPKSKNLD PNIYGGYSSN TNAYLAIIKI NKASESTYKV VSVPMRILGK LNQTQNVIEH
1201 DNLLKEYLAP TILDKRGVRD FSIVKGKVHY KQVVWDGNRK YMLGSATYLY NAKQLTLSTE
1261 AMRVVTGDFK TNDDESLLLD QVFDEILTKV DQYLPLFDVG KAREKLHHGR TKFYDLSVID
1321 KKYVVHQLLI GLHDNPAQGD TLKIGFSNGM KLGLMKLGSG ITLSPNTKLI YRSPTGLFEK
1381 RIKITDL
//