LOCUS       SIU02569.1               480 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 probable
            poly(a) polymerase pcna (polynucleotide adenylyltransferase)
            (ntp polymerase) (rna adenylating enzyme) (poly(a) polymerase)
            protein.
ACCESSION   LT708304-3983
PROTEIN_ID  SIU02569.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="pcnA"
                     /locus_tag="BQ2027_MB3937C"
                     /note="Mb3937c, pcnA, len: 480 aa. Equivalent to Rv3907c,
                     len: 480 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 480 aa overlap). Probable pcnA,
                     polynucleotide polymerase (EC 2.7.7.19), equivalent to
                     Q9CCY1|PCNA|ML2697 PCNA PROTEIN from Mycobacterium leprae
                     (486 aa), FASTA scores: opt: 2713, E(): 4.3e-160, (84.1%
                     identity in 478 aa overlap); and Q59534|PCNB POLYA
                     POLYMERASE from Mycobacterium leprae (411 aa) FASTA
                     scores: opt: 2077, E(): 7.1e-121, (82.55% identity in 373
                     aa overlap). Also highly similar to many e.g.
                     Q9X8T2|SCH24.18 PUTATIVE RNA NUCLEOTIDYLTRANSFERASE from
                     Streptomyces coelicolor (483 aa), FASTA scores: opt: 1856,
                     E(): 3.7e-107, (61.55% identity in 455 aa overlap); Q9ZN65
                     POLYA POLYMERASE from Prevotella ruminicola (Bacteroides
                     ruminicola) (479 aa), FASTA scores: opt: 830, E():
                     8.5e-44, (34.85% identity in 445 aa overlap);
                     P42977|PAPS_BACSU POLY(A) POLYMERASE from Bacillus
                     subtilis (397 aa), FASTA scores: opt: 479, E(): 3.5e-22,
                     (29.35% identity in 450 aa overlap); etc. Contains:
                     PS00017 ATP/GTP-binding site motif A (P-loop), PS00018
                     EF-hand calcium-binding domain, and probably less
                     significant a PS00237 G-protein coupled receptor
                     signature, and PS00639 Eukaryotic thiol (cysteine)
                     proteases histidine active site. BELONGS TO THE TRNA
                     NUCLEOTIDYLTRANSFERASE / POLY(A) POLYMERASE FAMILY.
                     Protein product from Mb3937c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb3937c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y5L0"
                     /db_xref="InterPro:IPR002646"
                     /db_xref="InterPro:IPR003607"
                     /db_xref="InterPro:IPR006674"
                     /db_xref="InterPro:IPR006675"
                     /db_xref="InterPro:IPR014065"
                     /db_xref="InterPro:IPR032828"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y5L0"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MPEAVQEADL LTAAAVALNR HAALLRELGS VFAAAGHELY LVGGSVRDAL LGRLSPDLDF
       61 TTDARPERVQ EIVRPWADAV WDTGIEFGTV GVGKSDHRME ITTFRADSYD RVSRHPEVRF
      121 GDCLEGDLVR RDFTTNAMAV RVTATGPGEF LDPLGGLAAL RAKVLDTPAA PSGSFGDDPL
      181 RMLRAARFVS QLGFAVAPRV RAAIEEMAPQ LARISAERVA AELDKLLVGE DPAAGIDLMV
      241 QSGMGAVVLP EIGGMRMAID EHHQHKDVYQ HSLTVLRQAI ALEDDGPDLV LRWAALLHDI
      301 GKPATRRHEP DGGVSFHHHE VVGAKMVRKR MRALKYSKQM IDDISQLVYL HLRFHGYGDG
      361 KWTDSAVRRY VTDAGALLPR LHKLVRADCT TRNKRRAARL QASYDRLEER IAELAAQEDL
      421 DRVRPDLDGN QIMAVLDIPA GPQVGEAWRY LKELRLERGP LSTEEATTEL LSWWKSRGNR
//