LOCUS SIU02569.1 480 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable poly(a) polymerase pcna (polynucleotide adenylyltransferase) (ntp polymerase) (rna adenylating enzyme) (poly(a) polymerase) protein. ACCESSION LT708304-3983 PROTEIN_ID SIU02569.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pcnA" /locus_tag="BQ2027_MB3937C" /note="Mb3937c, pcnA, len: 480 aa. Equivalent to Rv3907c, len: 480 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 480 aa overlap). Probable pcnA, polynucleotide polymerase (EC 2.7.7.19), equivalent to Q9CCY1|PCNA|ML2697 PCNA PROTEIN from Mycobacterium leprae (486 aa), FASTA scores: opt: 2713, E(): 4.3e-160, (84.1% identity in 478 aa overlap); and Q59534|PCNB POLYA POLYMERASE from Mycobacterium leprae (411 aa) FASTA scores: opt: 2077, E(): 7.1e-121, (82.55% identity in 373 aa overlap). Also highly similar to many e.g. Q9X8T2|SCH24.18 PUTATIVE RNA NUCLEOTIDYLTRANSFERASE from Streptomyces coelicolor (483 aa), FASTA scores: opt: 1856, E(): 3.7e-107, (61.55% identity in 455 aa overlap); Q9ZN65 POLYA POLYMERASE from Prevotella ruminicola (Bacteroides ruminicola) (479 aa), FASTA scores: opt: 830, E(): 8.5e-44, (34.85% identity in 445 aa overlap); P42977|PAPS_BACSU POLY(A) POLYMERASE from Bacillus subtilis (397 aa), FASTA scores: opt: 479, E(): 3.5e-22, (29.35% identity in 450 aa overlap); etc. Contains: PS00017 ATP/GTP-binding site motif A (P-loop), PS00018 EF-hand calcium-binding domain, and probably less significant a PS00237 G-protein coupled receptor signature, and PS00639 Eukaryotic thiol (cysteine) proteases histidine active site. BELONGS TO THE TRNA NUCLEOTIDYLTRANSFERASE / POLY(A) POLYMERASE FAMILY. Protein product from Mb3937c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3937c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y5L0" /db_xref="InterPro:IPR002646" /db_xref="InterPro:IPR003607" /db_xref="InterPro:IPR006674" /db_xref="InterPro:IPR006675" /db_xref="InterPro:IPR014065" /db_xref="InterPro:IPR032828" /db_xref="UniProtKB/TrEMBL:A0A1R3Y5L0" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MPEAVQEADL LTAAAVALNR HAALLRELGS VFAAAGHELY LVGGSVRDAL LGRLSPDLDF 61 TTDARPERVQ EIVRPWADAV WDTGIEFGTV GVGKSDHRME ITTFRADSYD RVSRHPEVRF 121 GDCLEGDLVR RDFTTNAMAV RVTATGPGEF LDPLGGLAAL RAKVLDTPAA PSGSFGDDPL 181 RMLRAARFVS QLGFAVAPRV RAAIEEMAPQ LARISAERVA AELDKLLVGE DPAAGIDLMV 241 QSGMGAVVLP EIGGMRMAID EHHQHKDVYQ HSLTVLRQAI ALEDDGPDLV LRWAALLHDI 301 GKPATRRHEP DGGVSFHHHE VVGAKMVRKR MRALKYSKQM IDDISQLVYL HLRFHGYGDG 361 KWTDSAVRRY VTDAGALLPR LHKLVRADCT TRNKRRAARL QASYDRLEER IAELAAQEDL 421 DRVRPDLDGN QIMAVLDIPA GPQVGEAWRY LKELRLERGP LSTEEATTEL LSWWKSRGNR //