LOCUS       SIU02559.1               329 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 CONSERVED
            HYPOTHETICAL PROTEIN protein.
ACCESSION   LT708304-3973
PROTEIN_ID  SIU02559.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /locus_tag="BQ2027_MB3927C"
                     /note="Mb3927c, -, len: 329 aa. Equivalent to Rv3898c and
                     Rv3897c, len: 110 aa and 210 aa, from Mycobacterium
                     tuberculosis strain H37Rv, (100.0% identity in 110 aa
                     overlap and 98.8% identity in 168 aa overlap). Conserved
                     hypothetical proteins. Highly similar, but in part, to
                     Q10691|YK83_MYCTU|Rv2083|MT2145|MTCY49.22 HYPOTHETICAL
                     30.8 KDA PROTEIN from Mycobacterium tuberculosis (314 aa)
                     FASTA scores: opt: 204, E(): 0.00042, (50.6% identity in
                     81 aa overlap) and FASTA scores: opt: 815, E(): 4.7e-26,
                     (73.05% identity in 167 aa overlap). Similarity suggests
                     it should be in frame with next ORF and that the stop
                     codon could be read through, the sequence appears to be
                     correct. Homology lost upstream at 15138 gatc sequence may
                     suggest discontinuity due to chimerism in cY15F10 or cY49.
                     Similarity to MTCY49.22 suggests that this is a
                     continuation of MTCY15F10.14. There is a frameshift
                     mutation near 3'-end with respect to this sequence as
                     well, similarity to MTCY49.22 continues in an overlapping
                     ORF. Sequence appears to be correct.
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis, Rv3898c and Rv3897c exist as 2 genes. In
                     Mycobacterium bovis, a single base transition (t-c) leads
                     to a single product. Protein product from Mb3927c detected
                     using SWATH mass spectrometry and 0. Mb3927c found to be
                     expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y5K4"
BEGIN
        1 MTGDQNPAPG PAPGVPIKVT PEILLQVLTT PPASGPAPFP AVPVDLPAPA DIANGALFAA
       61 GNSGVPGDVE SSGLEDLDRR AHAADAVQKF SANEADAAQQ FQGVGAQAEA QGATQMMQQA
      121 VSGITGALGG AVGGVMGPLT QLPQQAMQAG QGAMQPLMSA LQQTYGAEGL DVADGARLVD
      181 SIEGEPGLGG EPGAGDVGAG GGGGGTTPTG YLGPPPVPTS SPPTTPAGAP AKSVTPDPVS
      241 GTPRASGPAG MTGMPMVPPG ALGAGAEGAN KDKPVEKRVT APAVPNGQPV KGRLTVPPSV
      301 PVKSADDKPV VTKSTRRILV VPNDDKVKE
//