LOCUS SIU02555.1 561 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
CONSERVED MEMBRANE PROTEIN [SECOND PART] protein.
ACCESSION LT708304-3969
PROTEIN_ID SIU02555.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="eccc2"
/locus_tag="BQ2027_MB3923C"
/note="Mb3923c, -, len: 561 aa. Equivalent to 3' end of
Rv3894c, len: 1396 aa, from Mycobacterium tuberculosis
strain H37Rv, (100.0% identity in 561 aa overlap).
Possible conserved membrane protein (possible
transmembrane segments from aa ~37-85), similar to
Q9CD30|ML2535 HYPOTHETICAL PROTEIN from Mycobacterium
leprae (1329 aa), FASTA scores: opt: 652, E(): 2.2e-30,
(27.85% identity in 1425 aa overlap); Q9CDD7|ML0052
HYPOTHETICAL PROTEIN from Mycobacterium leprae (597 aa),
FASTA scores: opt: 537, E(): 6.6e-24, (27.5% identity in
585 aa overlap) (similarity only with C-terminal end);
Q9Z5I2|ML1543|MLCB596.28 POSSIBLE SPOIIIE-FAMILY MEMBRANE
PROTEIN from Mycobacterium leprae (1345 aa), FASTA scores:
opt: 523, E(): 8.6e-23, (31.65% identity in 1412 aa
overlap). Also similar to various proteins e.g.
O86653|SC3C3.20c ATP/GTP BINDING PROTEIN from Streptomyces
coelicolor (1321 aa), FASTA scores: opt: 973, E():
2.8e-49, (28.1% identity in 1409 aa); Q9L0T6|SCD35.15c
PUTATIVE CELL DIVISION-RELATED PROTEIN from Streptomyces
coelicolor(1525 aa), FASTA scores: opt: 524, E(): 8.3e-23,
(24.95% identity in 1450 aa overlap); Q9KE81|BH0975
HYPOTHETICAL PROTEIN from Bacillus halodurans (1489 aa),
FASTA scores: opt: 444, E(): 4.1e-18, (22.5% identity in
1346 aa overlap); etc. Also similar to AAK46103|MT1833
FTSK/SPOIIIE FAMILY PROTEIN from Mycobacterium
tuberculosis strain CDC1551 (1391 aa), FASTA scores: opt:
769, E(): 2.9e-37, (30.6% identity in 1434 aa overlap);
and other hypothetical proteins from Mycobacterium
tuberculosis e.g. O53689|Rv0284|MTV035.12 (1330 aa), FASTA
scores: opt: 634, E(): 2.5e-29, (28.2% identity in 1443 aa
overlap); O06264|Rv3447c|MTCY77.19c (1236 aa), FASTA
scores: opt: 632, E(): 3.1e-29, (28.75% identity in 1391
aa overlap); O69736|R3871|MTV027.06 (591 aa), FASTA
scores: opt: 588, E(): 6.6e-27, (27.75% identity in 605 aa
overlap) (similarity only with C-terminal end); etc.
Contains two possible (PS00017) ATP/GTP-binding sites
(P-loop) in central portion.
REMARK-M.bovis-M.tuberculosis: In Mycobacterium
tuberculosis, Rv3894c exists as a single gene. In
Mycobacterium bovis, a frameshift due to a single base
insertion (*-c), splits Rv3894c into 2 parts, Mb3923c and
Mb3924c. Protein product from Mb3923c detected using SWATH
mass spectrometry. Mb3923c found to be expressed during
exponential growth in Sauton's minimal media by
RNA-sequencing."
/db_xref="GOA:A0A1R3Y6Y5"
/db_xref="InterPro:IPR002543"
/db_xref="InterPro:IPR023837"
/db_xref="InterPro:IPR027417"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y6Y5"
BEGIN
1 MAAYRGKPWH VDYGQNPGLM FPVGVMDIPE ESQQVVHAVD ALRSNIIVVG AKQRGKTTTL
61 MALMCSAATM YTPERVTFFC IGGATMAQIG SLPHVTDIVS PKDAEGIERI LSTMDALIDA
121 REEAFRRAKI DMDGFRERRF GIGGDGVGGT DPTDAFGDVF VVLDDYDDLY AKDTLLGDRI
181 ISLSSRGPEY GVHLMCSAGG WIHGQRQSLL QNVTARIQLR LADPGESQMG HLSIESREAA
241 RRTLNRPGFG LTESLHELRI GVPALADPGT GELVGITDVG ARIADVAGVT KHASLQRLPQ
301 RVELSAIVEH EAVHQGGDDL SIAFAIGERH ELGPVPIKLR ESPGLMILGR QGCGKTTALV
361 AIGEAVMNRF SPQQAQLTLI DPKTAPHGLR DLHAPGYVRA YAYDQDEIDE VITELAQQIL
421 LPRLPPKGLS QEELRALKPW EGPRHFVLID DVQDLRPAQS YPQKPPVGAA LWKLMERARQ
481 VGLHVFSTRN SANWATMPMD PWVKSQTSAK VAQLYMDNDP QNRINRSVRA QTLPPGRGLL
541 VGADGDVEGI LVGYPSVPGE Q
//