LOCUS SIU02531.1 480 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 esx conserved component eccb1. esx-1 type vii secretion system protein. possible membrane protein. protein. ACCESSION LT708304-3945 PROTEIN_ID SIU02531.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="eccb1" /locus_tag="BQ2027_MB3899" /note="Mb3899, -, len: 480 aa. Equivalent to Rv3869, len: 480 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 480 aa overlap). Possible conserved membrane protein (has hydrophobic stretch near N-terminus), equivalent to O33088|ML0054|MLCB628.17c PUTATIVE MEMBRANE PROTEIN from Mycobacterium leprae (481 aa), FASTA scores: opt: 2489, E(): 8.3e-136, (75.75% identity in 478 aa overlap); and similar to others e.g. Q9Z5I3|ML1544|MLCB596.27 CONSERVED MEMBRANE PROTEIN from Mycobacterium leprae (506 aa), FASTA scores: opt: 739, E(): 3.9e-35, (33.65% identity in 490 aa overlap). Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. O05449|Rv3895c|MTCY15F10.17 (495 aa), FASTA scores: opt: 795, E(): 2.3e-38, (35.8% identity in 486 aa overlap); O53933|Rv1782|MTV049.04 (506 aa), FASTA scores: opt: 763, E(): 1.6e-36, (34.7% identity in 490 aa overlap); O06317|Rv3450c|MTCY13E12.03c (470 aa) FASTA scores: opt: 717, E(): 6.7e-34, (32.55% identity in 479 aa overlap); etc. Protein product from Mb3899 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3899 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y5K0" /db_xref="InterPro:IPR007795" /db_xref="InterPro:IPR042485" /db_xref="UniProtKB/TrEMBL:A0A1R3Y5K0" BEGIN 1 MGLRLTTKVQ VSGWRFLLRR LEHAIVRRDT RMFDDPLQFY SRSIALGIVV AVLILAGAAL 61 LAYFKPQGKL GGTSLFTDRA TNQLYVLLSG QLHPVYNLTS ARLVLGNPAN PATVKSSELS 121 KLPMGQTVGI PGAPYATPVS AGSTSIWTLC DTVARADSTS PVVQTAVIAM PLEIDASIDP 181 LQSHEAVLVS YQGETWIVTT KGRHAIDLTD RALTSSMGIP VTARPTPISE GMFNALPDMG 241 PWQLPPIPAA GAPNSLGLPD DLVIGSVFQI HTDKGPQYYV VLPDGIAQVN ATTAAALRAT 301 QAHGLVAPPA MVPSLVVRIA ERVYPSPLPD EPLKIVSRPQ DPALCWSWQR SAGDQSPQST 361 VLSGRHLPIS PSAMNMGIKQ IHGTATVYLD GGKFVALQSP DPRYTESMYY IDPQGVRYGV 421 PNAETAKSLG LSSPQNAPWE IVRLLVDGPV LSKDAALLEH DTLPADPSPR KVPAGASGAP //