LOCUS SIU02520.1 488 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable
nadh-dependent glutamate synthase (small subunit) gltd
(l-glutamate synthase) (l-glutamate synthetase) (nadh-
glutamate synthase) (glutamate synthase (nadh)) (glts
beta chain) (nadph-gogat) protein.
ACCESSION LT708304-3934
PROTEIN_ID SIU02520.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="gltD"
/locus_tag="BQ2027_MB3888C"
/note="Mb3888c, gltD, len: 488 aa. Equivalent to Rv3858c,
len: 488 aa, from Mycobacterium tuberculosis strain H37Rv,
(99.8% identity in 488 aa overlap). Probable gltD, small
subunit of NADH-dependent glutamate synthase (EC
1.4.1.13), equivalent to Q9CDD4|GLTD|ML0062 NADH-DEPENDENT
GLUTAMATE SYNTHASE SMALL SUBUNIT from Mycobacterium leprae
(488 aa), FASTA scores: opt: 2997, E(): 1e-166, (87.7%
identity in 488 aa overlap). Also highly similar to many
e.g. Q9S2Z0|SC3A3.03s from Streptomyces coelicolor (487
aa), FASTA scores: opt: 2152, E(): 1.2e-117, (63.85%
identity in 487 aa overlap); Q9KPJ3|VC2374 from Vibrio
cholerae (489 aa), FASTA scores: opt: 1699, E(): 2.5e-91,
(51.75% identity in 487 aa overlap); Q03460|GLSN_MEDSA
from Medicago sativa (Alfalfa) (2194 aa), FASTA scores:
opt: 1322, E(): 6.2e-69, (54.45% identity in 485 aa
overlap); P09832|GLTD_ECOLI from strain (471 aa) FASTA
scores: opt: 889, E() : 0, (37.4% identity in 473 aa
overlap); etc. SIMILAR TO OTHER GLUTAMATE SYNTHASES.
COFACTOR: FAD (BY SIMILARITY). Protein product from
Mb3888c detected using shotgun mass spectrometry and SWATH
mass spectrometry. Mb3888c found to be expressed during
exponential growth in Sauton's minimal media by
RNA-sequencing."
/db_xref="GOA:A0A1R3Y5G4"
/db_xref="InterPro:IPR006005"
/db_xref="InterPro:IPR009051"
/db_xref="InterPro:IPR023753"
/db_xref="InterPro:IPR028261"
/db_xref="InterPro:IPR036188"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y5G4"
BEGIN
1 MADPGGFLKY THRKLPKRRP VPLRLRDWRE VYEEFDNESL RQQATRCMDC GIPFCHNGCP
61 LGNLIPEWND LVRRGRWRDA IERLHATNNF PDFTGRLCPA PCEPACVLGI NQDPVTIKQI
121 ELEIIDKAFD EGWVQPRPPR KLTGQTVAVV GSGPAGLAAA QQLTRAGHTV TVFEREDRIG
181 GLLRYGIPEF KMEKRHLDRR LDQMRSEGTE FRPGVNVGVD ISAEKLRADF DAVVLAGGAT
241 AWRELPIPGR ELEGVHQAME FLPWANRVQE GDDVLDEDGQ PPITAKGKKV VIIGGGDTGA
301 DCLGTVHRQG AIAVHQFEIM PRPPDARAES TPWPTYPLMY RVSAAHEEGG ERVFSVNTEA
361 FVGTDGRVSA LRAHEVTMLD GKFVKVEGSD FELEADLVLL AMGFVGPERA GLLTDLGVKF
421 TEHGNVARGD DFDTSVPGVF VAGDMGRGQS LIVWAIAEGR AAAAAVDRYL MGSSALPAPV
481 KPTAAPLQ
//