LOCUS SIU02468.1 399 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 UDP- GALACTOPYRANOSE MUTASE GLF (UDP-GALP MUTASE) (NAD+-FLAVIN ADENINE DINUCLEOTIDE-REQUIRING ENZYME) protein. ACCESSION LT708304-3882 PROTEIN_ID SIU02468.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="glf" /locus_tag="BQ2027_MB3839C" /standard_name="ceoA" /note="Mb3839c, glf, len: 399 aa. Equivalent to Rv3809c, len: 399 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 399 aa overlap). glf (alternate gene name: ceoA), UDP-galactopyranose mutase (EC 5.4.99.9) (see citations below), identical to previously sequenced gene, and equivalent to Q9CDB8|GLF|ML0092 PUTATIVE UDP-GALACTOPYRANOSE MUTASE from Mycobacterium leprae (413 aa), FASTA scores: opt: 2347, E(): 1.3e-140, (86.6% identity in 396 aa overlap). Also highly similar to others e.g. AAK61905|EPSJ UDP-GALACTOPYRANOSE MUTASE (PROTEIN INVOLVED IN EXOPOLYSACCHARIDES BIOSYNTHESIS) from Streptococcus thermophilus (365 aa), FASTA scores: opt: 972, E(): 5.9e-54, (45.85% identity in 375 aa overlap); P37747|GLF_ECOLI|B2036 UDP-GALACTOPYRANOSE MUTASE from Escherichia coli strain K12 (367 aa), FASTA scores: opt: 958, E(): 4.5e-53, (43.55% identity in 379 aa overlap); O86897|CAP33FN from Streptococcus pneumoniae (369 aa) FASTA scores: opt: 954, E(): 8.1e-53, (44.8% identity in 375 aa overlap); etc. COFACTOR: FAD (BY SIMILARITY). N-TERMINAL SHOWS SIMILARITY TO FAD OR NAD CONTAINING PROTEINS. Protein product from Mb3839c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3839c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y5B9" /db_xref="InterPro:IPR004379" /db_xref="InterPro:IPR015899" /db_xref="UniProtKB/TrEMBL:A0A1R3Y5B9" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MQPMTARFDL FVVGSGFFGL TIAERVATQL DKRVLVLERR PHIGGNAYSE AEPQTGIEVH 61 KYGAHLFHTS NKRVWDYVRQ FTDFTDYRHR VFAMHNGQAY QFPMGLGLVS QFFGKYFTPE 121 QARQLIAEQA AEIDTADAQN LEEKAISLIG RPLYEAFVKG YTAKQWQTDP KELPAANITR 181 LPVRYTFDNR YFSDTYEGLP TDGYTAWLQN MAADHRIEVR LNTDWFDVRG QLRPGSPAAP 241 VVYTGPLDRY FDYAEGRLGW RTLDFEVEVL PIGDFQGTAV MNYNDLDVPY TRIHEFRHFH 301 PERDYPTDKT VIMREYSRFA EDDDEPYYPI NTEADRALLA TYRARAKSET ASSKVLFGGR 361 LGTYQYLDMH MAIASALNMY DNVLAPHLRD GVPLLQDGA //