LOCUS       SIU02442.1               326 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 possible
            dtdp-glucose 4,6-dehydratase protein.
ACCESSION   LT708304-3856
PROTEIN_ID  SIU02442.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="rfbB"
                     /locus_tag="BQ2027_MB3813"
                     /note="Mb3813, rfbB, len: 326 aa. Equivalent to Rv3784,
                     len: 326 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 326 aa overlap). Possible rfbB,
                     dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), similar to
                     others e.g. Q9YCT1|APE1180 LONG HYPOTHETICAL DTDP-GLUCOSE
                     4,6-DEHYDRATASE from Aeropyrum pernix (330 aa) FASTA
                     scores: opt: 598, E(): 3.7e-30, (34.9% identity in 315 aa
                     overlap); O27817|MTH1789 DTDP-GLUCOSE 4,6-DEHYDRATASE from
                     Methanothermobacter thermautotrophicus (336 aa) FASTA
                     scores: opt: 587, E(): 1.8e-29, (34.9% identity in 315 aa
                     overlap); Q9X5W0|GRSE TDP-GLUCOSE-4,6-DEHYDRATASE HOMOLOG
                     from Streptomyces griseus (324 aa), FASTA scores: opt:
                     583, E(): 3.2e-29, (35.7% identity in 325 aa overlap);
                     Q9K7J7|SPSJ|BH3364 SPORE COAT POLYSACCHARIDE SYNTHESIS
                     (DTDP GLUCOSE 4, 6-DEHYDRATASE) from Bacillus halodurans
                     (321 aa), FASTA scores: opt: 562, E(): 6.5e-28, (33.0%
                     identity in 318 aa overlap); Q9UZH2|RFBB|PAB0785
                     DTDP-GLUCOSE 4,6-DEHYDRATASE from Pyrococcus abyssi (333
                     aa), FASTA scores: opt: 552, E(): 2.8e-27, (33.95%
                     identity in 318 aa overlap); P27830|RFFG_ECOLI|B3788
                     DTDP-GLUCOSE 4,6-DEHYDRATASE from Escherichia coli strain
                     K12 (355 aa), FASTA scores: opt: 401, E(): 7.5e-28, (31.3%
                     identity in 348 aa overlap); etc. But also similar to
                     several UDP-glucose 4-epimerases (EC 5.1.3.2) and other
                     proteins e.g. O59375|PH1742 LONG HYPOTHETICAL UDP-GLUCOSE
                     4-EPIMERASE from Pyrococcus horikoshii (306 aa) FASTA
                     scores: opt: 600, E(): 2.6e-30, (34.5% identity in 313 aa
                     overlap); Q9ZGC7|LANH14 NDP-HEXOSE 4,6-DEHYDRATASE
                     HOMOLOGfrom Streptomyces cyanogenus (326 aa), FASTA
                     scores: opt: 593, E(): 7.6e-30, (36.45% identity in 321 aa
                     overlap); Q57664|GALE_METJA|MJ0211 PUTATIVE UDP-GLUCOSE
                     4-EPIMERASE from Methanococcus jannaschii (305 aa) FASTA
                     scores: opt: 575, E(): 9.6e-29, (32.6% identity in 313 aa
                     overlap); etc. SEEMS TO BELONG TO THE SUGAR EPIMERASE
                     FAMILY, DTDP-GLUCOSE DEHYDRATASE SUBFAMILY. Note that
                     previously known as epiB. Protein product from Mb3813
                     detected using SWATH mass spectrometry. Mb3813 found to be
                     expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR036291"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y576"
BEGIN
        1 MEILVTGGAG FQGSHLTESL LANGHWVTVL DKSSRNAVRN MQGFRSHDRA AFISGSVTDG
       61 QTIDRAVRDH HVVFHLAAHV NVDQSLGDPE SFLETNVMGT YRVLEAVRRY RNRLIYVSTC
      121 EVYGDGHNLK EGERLDEHAE LKPNSPYGAS KAAADRLCYS YFRSYGLDVT IVRPFNIFGV
      181 RQKAGRFGAL IPRLVRQGIN GEGLTIFGAG SATRDYLYVS DIVGAYNLVL RTPTLRGQAI
      241 NFASGKDTRV RDIVEYVADK FGARIEHRDA RPGEVQRFPA DISLAKSIGF QPQVEIWDGI
      301 DRYINWAKDQ PQYPYEQDGF SGSSVL
//