LOCUS SIU02439.1 273 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE O-ANTIGEN/LIPOPOLYSACCHARIDE TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER RFBE protein. ACCESSION LT708304-3853 PROTEIN_ID SIU02439.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="rfbE" /locus_tag="BQ2027_MB3810" /note="Mb3810, rbfE, len: 273 aa. Equivalent to Rv3781, len: 273 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 273 aa overlap). Probable rfbE, polysaccharide-transport ATP-binding protein ABC transporter (see citation below), involved in O-antigen/lipopolysaccharides (LPS) transport, equivalent to Q9CDA0|ML0114 PUTATIVE ABC TRANSPORTER ATP-BINDING COMPONENT from Mycobacterium leprae (272 aa), FASTA scores: opt: 1581, E(): 3e-83, (91.4% identity in 267 aa overlap). Also highly similar to AAK71283 LPS/O-ANTIGEN EXPORT PERMEASE from Coxiella burnetii (258 aa), FASTA scores: opt: 793, E(): 2.5e-38, (45.45% identity in 253 aa overlap); Q9PAF0|XF2568 ABC TRANSPORTER ATP-BINDING PROTEIN from Xylella fastidiosa (246 aa), FASTA scores: opt: 758, E(): 2.4e-36, (47.75% identity in 243 aa overlap); Q56903|RFBE_YEREN O-ANTIGEN EXPORT SYSTEM ATP-BINDING PROTEIN from Yersinia enterocolitica (239 aa), FASTA scores: opt: 697, E(): 7e-33, (48.65% identity in 224 aa overlap); Q50863|RFBB_MYXXA O-ANTIGEN EXPORT SYSTEM ATP-BINDING from Myxococcus xanthus (437 aa), FASTA scores: opt: 605, E(): 2e-27, (42.05% identity in 207 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). BELONGS TO THE ATP-BINDING TRANSPORT PROTEIN FAMILY (ABC TRANSPORTERS). Protein product from Mb3810 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3810 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y583" /db_xref="InterPro:IPR003439" /db_xref="InterPro:IPR003593" /db_xref="InterPro:IPR027417" /db_xref="UniProtKB/TrEMBL:A0A1R3Y583" BEGIN 1 MSDPHHPHIQ THNAWVEFPI FDAKSRSLKK AVLGKAGGTI GRNNSNVVVI EALRDITMEL 61 NLGDRVGLVG HNGAGKSTLL RLLSGIYEPT RGWAKVTGRV APVFDLGIGM DPEISGYENI 121 IIRGLFLGQT RKQMQAKVDE IAEFTELGEY LSMPLRTYST GMRVRLAMGV VTSIDPEILL 181 LDEGIGAVDA DFLRKAQSRL QNLVERSGIL VFASHSNEFL ARLCKTAIWI DHGVIRLAGG 241 IEEVVRAYEG EDAARHVREV LAETQADRQN VQG //