LOCUS SIU02433.1 415 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable lipase lipe protein. ACCESSION LT708304-3847 PROTEIN_ID SIU02433.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="lipE" /locus_tag="BQ2027_MB3804" /note="Mb3804, lipE, len: 415 aa. Equivalent to Rv3775, len: 415 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 415 aa overlap). Probable lipE, hydrolase lipase (EC 3.1.-.-), equivalent to Q9CD95|LIPE|ML0119 PROBABLE HYDROLASE from Mycobacterium leprae (411 aa), FASTA scores: opt: 2418, E(): 6.4e-144, (84.75% identity in 406 aa overlap). Also similar to other esterases e.g. Q9ABH2|CC0255 ESTERASE A from Caulobacter crescentus (374 aa), FASTA scores: opt: 427, E(): 2.4e-19, (28.9% identity in 391 aa overlap); O87861|ESTA ESTERASE A from Streptomyces chrysomallus (389 aa), FASTA scores: opt: 417, E(): 1e-18, (31.0% identity in 361 aa overlap); Q9RK50|SCF12.08 PUTATIVE ESTERASE from Streptomyces coelicolor (376 aa), FASTA scores: opt: 385, E(): 1e-16, (31.35% identity in 373 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. P71778|Rv1497|MTCY277.19 HYPOTHETICAL 45.8 KDA PROTEIN (429 aa), FASTA scores: opt: 457, E(): 3.5e-21, (30.4% identity in 395 aa overlap). Protein product from Mb3804 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3804 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR001466" /db_xref="InterPro:IPR012338" /db_xref="UniProtKB/TrEMBL:A0A1R3Y568" BEGIN 1 MRAGDGKIRV PADLDAVTAT GEEDHSEIDG AAVDRIWRAA RHWYRAGMHP AIQLCIRHHG 61 RVVLNRAIGH GWGNAPTDEA DAEKIPVTTD TPFCVYSAAK AITATVVHML VERGHFALDD 121 RVCEYLPSYT SHGKHRTTIR HVLTHSAGVP FPTGPRPDVR RADDHEYAVE RLGELRPLYR 181 PGLVHIYHAL TWGPLMREIV YAATGKEIRE ILATEILDPL GFRWTNFGVA ERDVPLVAPS 241 HATGRQLPPV IAAVFRKAIG GTVHEIIPYT NTPFFLSTIL PSSNTVSTAN ELSRFMEILR 301 RGGELDGVRV LSPETLRGAV TECRRLRPDF ATGLMPLRWG TGFMLGSAKY GPFGRNAPAA 361 FGHLGLVNIA VWADPERALS GGLISSGKPG RDPEAGRYGA LLNAITAEIP RASSG //