LOCUS SIU02419.1 475 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 possible two component sensor kinase tcry protein. ACCESSION LT708304-3833 PROTEIN_ID SIU02419.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="tcry" /locus_tag="BQ2027_MB3790C" /note="Mb3790c, -, len: 475 aa. Equivalent to Rv3764c, len: 475 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 475 aa overlap). Possible histidine protein kinase (EC 2.7.3.-), part of a two-component regulatory system, similar to others e.g. Q9ADN6|2SC10A7.25 PUTATIVE TWO COMPONENT SYSTEM HISTIDINE KINASE from Streptomyces coelicolor (524 aa), FASTA scores: opt: 1332, E(): 5.4e-70, (49.9% identity in 477 aa overlap); Q9L3C1|KB|CAC42479 PUTATIVE HISTIDINE KINASE from Amycolatopsis mediterranei (469 aa), FASTA scores: opt: 515, E(): 1.4e-22, (36.1% identity in 313 aa overlap); P72560 HISTIDINE PROTEIN KINASE from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (438 aa), FASTA scores: opt: 480, E(): 1.4e-20, (40.1% identity in 232 aa overlap); P30847|P76401|BAES_ECOLI|B2078 SENSOR PROTEIN from Escherichia coli strain K12 (467 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. P96368|Rv1032c|MTCY10G2.17 (509 aa), FASTA scores: opt: 1007, E(): 4e-51, (43.5% identity in 416 aa overlap); and P71815|Rv0758|MTCY369.03 (485 aa), FASTA scores: opt: 738, E(): 1.6e-35, (28.6% identity in 438 aa overlap). Equivalent to AAK48235 from Mycobacterium tuberculosis strain CDC1551 (506 aa) but shorter 31 aa. Protein product from Mb3790c detected using SWATH mass spectrometry. Mb3790c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y574" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR003660" /db_xref="InterPro:IPR003661" /db_xref="InterPro:IPR004358" /db_xref="InterPro:IPR005467" /db_xref="InterPro:IPR036097" /db_xref="InterPro:IPR036890" /db_xref="UniProtKB/TrEMBL:A0A1R3Y574" BEGIN 1 MGITAATEMA LRRHLVAQLD NQLGGTSYRS VLMYPEKMPR PPWRRETHNY IRSGPGPRFL 61 DAPGQPAGMV AAVVSDGTTV AAGYLTGSGS RAALTSTGRS QLERIAGSRT PLTLDLDGLG 121 RYRVLAAPSR NGHDVIVTGL SMGNVDATML QMLIIFGIVT VIALVAATTA GIVIIKRALA 181 PLRRVAQTAS EVVDLPLDRG EVKLPVRVPE PDANPSTEVG QLGSALNRML DHIAAALSAR 241 QASETRVRQF VADASHELRT PLAAIRGYTE LTQRIGDDPE AVAHAMSRVA SETERITRLV 301 EDLLLLARLD SGRPLERGPV DMSRLAVDAV SDAHVAGPDH QWALDLPPEP VVIPGDAARL 361 HQVVTNLLAN ARVHTGPGTI VTTRLSTGPT HVVLQVIDNG PGIPAALQSE VFERFARGDT 421 SRSRQAGSTG LGLAIVSAVV KAHNGTITVS SSPGYTEFAV RLPLDGWQPL ESSPR //