LOCUS       SIU02414.1               315 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            OSMOPROTECTANT (GLYCINE BETAINE/CARNITINE/CHOLINE/L-PROLINE)
            BINDING LIPOPROTEIN PROX protein.
ACCESSION   LT708304-3828
PROTEIN_ID  SIU02414.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="proX"
                     /locus_tag="BQ2027_MB3785C"
                     /note="Mb3785c, proX, len: 315 aa. Equivalent to Rv3759c,
                     len: 315 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.4% identity in 315 aa overlap). Possible proX,
                     osmoprotectant-binding lipoprotein component of
                     osmoprotectant transport system (see citation below),
                     similar to osmoprotection proteins (proX) involved in
                     glycine betaine/L-proline/choline transport, e.g.
                     AAK79442|CAC1474 PROLINE/GLYCINE BETAINE ABC TRANSPORT
                     SYSTEM PERIPLASMIC COMPONENT from Clostridium
                     acetobutylicum (303 aa), FASTA scores: opt: 308, E():
                     1.2e-11, (27.4% identity in 314 aa overlap);
                     Q9X4J2|PROXL|SCE19A.33 PROXL PROTEIN from Streptomyces
                     coelicolor (322 aa), FASTA scores: opt: 302, E(): 3e-11,
                     (27.2% identity in 327 aa overlap); O29280|AF0982
                     OSMOPROTECTION PROTEIN (PROX) from Archaeoglobus fulgidus
                     (292 aa), FASTA scores: opt: 235, E(): 3.4e-07, (23.15%
                     identity in 285 aa overlap); etc. Also similar to
                     MTV006_16 HYPOTHETICAL PROTEIN from Mycobacterium
                     tuberculosis, and MLU15180_43 HYPOTHETICAL PROTEIN from
                     Mycobacterium leprae. Equivalent to AAK48230 from
                     Mycobacterium tuberculosis strain CDC1551 (343 aa) but
                     shorter 28 aa. Contains probable N-terminal signal
                     sequence. Protein product from Mb3785c detected using
                     SWATH mass spectrometry. Mb3785c found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y542"
                     /db_xref="InterPro:IPR007210"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y542"
BEGIN
        1 MRMLRRLRRA TVAAAVWLAT VCLVASCANA DPLGSATGSV KSIVVGSGDF PESQVIAEIY
       61 AQVLQANGFD VGRRLGIGSR ETYIPALKDH SIDLVPEYIG NLLLYFQPDA TVTMLDAVEL
      121 ELYKRLPGDL SILTPSPASD TDTVTVTAAT AARWNLKTIA DLAPHSADVK FAAPSVFQTR
      181 PSGLPGLRHK YSLDIAPGNF VTINDGGGAV TVRALVEGTA TAANLFSTSA AIPQNHLVVL
      241 EDPEHNFLAG NIVPLVNSRK KSDHLKDVLD AVSAKLTTAG LAELNAAVSG NSGVDPDQAA
      301 RKWVRDNGFD HPVRQ
//