LOCUS       SIU02394.1               118 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 ppe
            family protein ppe66 protein.
ACCESSION   LT708304-3808
PROTEIN_ID  SIU02394.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="PPE66"
                     /locus_tag="BQ2027_MB3765C"
                     /note="Mb3765c, PPE66, len: 118 aa. Equivalent to 3' end
                     of Rv3738c, len: 315 aa, from Mycobacterium tuberculosis
                     strain H37Rv, (98.1% identity in 105 aa overlap). Member
                     of the Mycobacterium tuberculosis PPE family, highly
                     similar to many e.g. O53265|Rv3018c|MTV012.32c (434 aa),
                     FASTA scores: opt: 464, E(): 2.2e-17, (47.05% identity in
                     338 aa overlap). Probably a continuation of the upstream
                     ORF MTV025.87c. At position 97470-72 a stop codon is
                     present which interrupts a possibly longer ORF, observed
                     in related ORFs MTV012_32 or MTCY21B4_4. The sequence has
                     been checked and no errors were detected. A similar
                     situation, but with a frameshift separating the ORFs is
                     found in MTV012_36/MTV012_35. Sequence similarity is also
                     seen with MTCY251_15; MTCY261_19; MLCB2492_30 from
                     Mycobacterium leprae; MTCY10G2_10; MTY21C12_9; MTCI125_26;
                     MTCY164_36; MTCY6A4_1. REMARK-M.bovis-M.tuberculosis: In
                     Mycobacterium bovis, a deletion of 1166 bp leads to the
                     loss of the NH2 part of PPE66 and of Rv3739c compared to
                     its homolog in Mycobacterium tuberculosis strain H37Rv.
                     Mb3765c found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y518"
BEGIN
        1 MTKASPVYLP GVKGRITADV APVAVRPAAA PPLRESAAVR PEARLVSAVA PAPAGTSASV
       61 LASDRGAGVL GFAGTAGKES VGRPAGLTTL AGGEFGGSPS VPMVPASWEQ LVGAGEAG
//