LOCUS SIU02387.1 358 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 possible atp-dependent dna ligase ligc (polydeoxyribonucleotide synthase [atp]) (polynucleotide ligase [atp]) (sealase) (dna repair protein) (dna joinase) protein. ACCESSION LT708304-3801 PROTEIN_ID SIU02387.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ligC" /locus_tag="BQ2027_MB3758" /note="Mb3758, ligC, len: 358 aa. Equivalent to Rv3731, len: 358 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 358 aa overlap). Possible ligC, DNA ligase ATP-dependent (EC 6.5.1.1), similar to numerous archaebacterial and eukaryotic polynucleotide DNA ligases e.g. Q9XAM3|SC4C6.17c from Streptomyces coelicolor (355 aa), FASTA scores: opt: 1429, E(): 1.7e-82, (60.4% identity in 361 aa overlap); BAB54870|MLL9685 from Rhizobium loti (Mesorhizobium loti) plasmid pMLb (337 aa), FASTA scores: opt: 667, E(): 1.2e-34, (40.35% identity in 347 aa overlap); Q9HH07|DNLI_THEFM|LIG from Thermococcus fumicolans (559 aa), FASTA scores: opt: 335, E(): 1.4e-13, (27.25% identity in 330 aa overlap); O59288|DNLI_PYRHO from Pyrococcus horikoshii (559 aa), FASTA scores: opt: 307, E(): 8e-12, (26.85% identity in 272 aa overlap); etc. Also similar to Rv3062|MTCY22D7_19c|LIGB PROBABLE DNA LIGASE from Mycobacterium tuberculosis (507 aa), FASTA score: (30.3% identity in 356 aa overlap). SEEMS TO BELONG TO THE ATP-DEPENDENT DNA LIGASE FAMILY. Protein product from Mb3758 detected using SWATH mass spectrometry. Mb3758 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y525" /db_xref="InterPro:IPR012309" /db_xref="InterPro:IPR012310" /db_xref="InterPro:IPR012340" /db_xref="UniProtKB/TrEMBL:A0A1R3Y525" BEGIN 1 MQLPVMPPVS PMLAKSVTAI PPDASYEPKW DGFRSICFRD GDQVELGSRN ERPMTRYFPE 61 LVAAIRAELP HRCVIDGEII IATDHGLDFE ALQQRIHPAE SRVRMLADRT PASFIAFDLL 121 ALGDDDYTGR PFSERRAALV DAVTGSGADA DLSIHVTPAT TDMATAQRWF SEFEGAGLDG 181 VIAKPPHITY QPDKRVMFKI KHLRTADCVV AGYRVHKSGS DAIGSLLLGL YQEDGQLASV 241 GVIGAFPMAE RRRLLTELQP LVTSFDDHPW NWAAHVAGQR TPRKNEFSRW NVGKDLSFVP 301 LRPERVVEVR YDHMEGARFR HTAQFNRWRP DRDPRSCSYA QLERPLTVSL SDIVPGLR //