LOCUS SIU02386.1 346 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ATP-
dependent DNA ligase (EC protein.
ACCESSION LT708304-3800
PROTEIN_ID SIU02386.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/locus_tag="BQ2027_MB3757C"
/EC_number="6.5.1.1"
/note="Mb3757c, -, len: 346 aa. Equivalent to Rv3730c,
len: 346 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 346 aa overlap). Conserved
hypothetical protein, highly similar to Q9XAM1|SC4C6.19
HYPOTHETICAL 38.5 KDA PROTEIN from Streptomyces coelicolor
(341 aa), FASTA scores: opt: 1313, E(): 2.2e-75, (59.25%
identity in 336 aa overlap); and similar to C-terminal end
of PUTATIVE ATP-DEPENDENT DNA LIGASES e.g.
BAB49297|MLL2077 from Rhizobium loti (Mesorhizobium loti)
(833 aa), FASTA scores: opt: 550, E(): 5.3e-27, (31.3%
identity in 294 aa overlap); and BAB54816|MLL9625 from
Rhizobium loti (Mesorhizobium loti) plasmid pMLb (883 aa)
FASTA scores: opt: 492, E(): 2.5e-23, (33.7% identity in
291 aa overlap); etc. Also similar to the hypothetical
proteins e.g. Q9ZC15|SC1E6.07 HYPOTHETICAL 34.9 KDA
PROTEIN from Streptomyces coelicolor (319 aa) FASTA
scores: opt: 537, E(): 1.5e-26, (34.95% identity in 292 aa
overlap); Q9XAF7|SC6G9.25 HYPOTHETICAL 32.1 KDA PROTEIN
from Streptomyces coelicolor (293 aa), FASTA scores: opt:
474, E(): 1.3e-22, (33.75% identity in 302 aa overlap);
etc. Also highly similar to P95226|Rv0269c|MTCY06A4.13c
HYPOTHETICAL 44.0 KDA PROTEIN from Mycobacterium
tuberculosis (397 aa), FASTA scores: opt: 940, E():
7.7e-52, (50.3% identity in 312 aa overlap). Protein
product from Mb3757c detected using SWATH mass
spectrometry. Mb3757c found to be expressed during
exponential growth in Sauton's minimal media by
RNA-sequencing."
/db_xref="InterPro:IPR014145"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y5E6"
BEGIN
1 MAAAAEELDV DGIAVRLTSP DRMYFPKLGS HGTKRRLVEY YFAVAGGPML TALRDRPTHL
61 QRFPDGVDGE QIYQKRIPRH RPDYLQTCRV TFPSGRMADA LKVTHPAAIV WAAQMGTITL
121 HPWQVRCPDT EHPDELRIDL DPQPGTGFVE ARTVAVDVLR SVLDDLGLVG YPKTSGGRGI
181 HVFLRIATDW DFVEVRRAGI ALAREVERRA PDAVTTSWWK EERGARIFID FNQNARDRTM
241 ASAYSVRPTP IATVSMPLTW EELAGADPDD YTMTTVPELV KIRDDPWAGM DDVAQSIAPL
301 LDLAAADEER GLGDMPYPPN YPKMPGEPKR VQPSRDTDLK GGNTSK
//