LOCUS SIU02381.1 333 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 possible oxidoreductase protein. ACCESSION LT708304-3795 PROTEIN_ID SIU02381.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3752" /note="Mb3752, -, len: 333 aa. Equivalent to Rv3725, len: 309 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 250 aa overlap). Possible reductase (EC 1.-.-.-), similar to various oxidoreductases and hypothetical proteins e.g. O34285|HPNA HPNA PROTEIN from Zymomonas mobilis (337 aa), FASTA scores: opt: 317, E(): 6.1e-11, (30.5% identity in 272 aa overlap); Q9SZB3|F17M5.120|AT4G33360|AAK49584 HYPOTHETICAL 37.9 KDA PROTEIN from Arabidopsis thaliana (Mouse-ear cress) (344 aa), FASTA scores: opt: 314, E(): 9.1e-11, (30.35% identity in 267 aa overlap); AAK59445|AT4G33360 PUTATIVE DIHYDROKAEMPFEROL 4-REDUCTASE from Arabidopsis thaliana (Mouse-ear cress) (332 aa), FASTA scores: opt: 313, E(): 1e-10, (30.8% identity in 263 aa overlap); Q9FSC6|CCR CINNAMOYL-COA REDUCTASE (EC 1.2.1.44) from Populus trichocarpa (Western balsam poplar) (338 aa), FASTA scores: opt: 305, E(): 2.9e-10, (30.3% identity in 274 aa overlap); Q9M631 CINNAMOYL CoA REDUCTASE from Populus tremuloides (Quaking aspen) (337 aa), FASTA scores: opt: 291, E(): 1.8e-09, (30.15% identity in 272 aa overlap); P73212|DFRA_SYNY3|LR1706 PUTATIVE DIHYDROFLAVONOL-4-REDUCTASE (EC 1.1.1.219) (DIHYDROKAEMPFEROL 4-REDUCTASE) from Synechocystis sp. strain PCC 6803 (343 aa), FASTA scores: opt: 278, E(): 1e-08, (29.35% identity in 259 aa overlap); etc. Also some similarity to proteins from Mycobacterium tuberculosis e.g. P96816|Rv0139|MTCI5.13 HYPOTHETICAL PROTEIN (340 aa) FASTA scores: opt: 234, E(): 3.2e-06, (28.25% identity in 269 aa overlap); and O06373|galE1|Rv3634c|MTCY15C10.18 PROBABLE UDP-GLUCOSE 4-EPIMERASE (314 aa) (27.3% identity in 194 aa overlap). REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a single base insertion (*-a) leads to a longer product with a different COOH part compared to its homolog in Mycobacterium tuberculosis (333 aa versus 309 aa). Protein product from Mb3752 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3752 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y527" /db_xref="InterPro:IPR001509" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3Y527" BEGIN 1 MQNATMRVLV TGGTGFVGGW TAKAIADAGH SVRFLVRNPA RLKTSVAKLG VDVSDFAVAD 61 ISDRDSVREA LNGCDAVVHS AALVATDPRE TSRMLSTNMA GAQNVLGQAV ELGMDPIVHV 121 SSFTALFRPN LATLSADLPV AGGTDGYGQS KAQIEIYARG LQDAGAPVNI TYPGMVLGPP 181 VGDQFGEAGE GVRSALWMHV IPGRGAAWLI VDVRDVAALH AALLESGRGP RRYTAGGHRI 241 PVPELAKILG EVAGTTMLAV PVPDSALRVA GSVLDQAGPY LPFNTPFTAA GMQYYTQMPE 301 PDDSPSEKEL GITYRDPRDT VADTVTALRG LGS //