LOCUS SIU02376.1 420 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE FATTY ACID SYNTHASE protein. ACCESSION LT708304-3790 PROTEIN_ID SIU02376.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3747" /note="Mb3747, -, len: 420 aa. Equivalent to Rv3720, len: 420 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 420 aa overlap). Possible fatty-acyl-phospholipid synthase (EC 2.1.1.-), equivalent to Q9CB74|ML2334 (alias O69515|MLCB2407.16c, 439 aa) HYPOTHETICAL PROTEIN from Mycobacterium leprae (420 aa) FASTA scores: opt: 2508, E(): 4.7e-153, (86.45% identity in 420 aa overlap). Also similar (especially at the C-terminus) to various fatty-acid synthases (principally cyclopropane-fatty-acyl-phospholipid synthases (EC 2.1.1.79)) and hypothetical proteins e.g. Q9KZ58|SCE25.32c PUTATIVE FATTY ACID SYNTHASE from Streptomyces coelicolor (438 aa), FASTA scores: opt: 1101, E(): 5.5e-63, (46.1% identity in 425 aa overlap); P31049|YLP3_PSEPU HYPOTHETICAL 44.7 KDA PROTEIN from Pseudomonas putida (394 aa), FASTA scores: opt: 810, E(): 2.1e-44, (46.4% identity in 293 aa overlap); Q9HT28|PA5546 HYPOTHETICAL PROTEIN from Pseudomonas aeruginosa (394 aa), FASTA scores: opt: 804, E(): 5.2e-44, (40.7% identity in 371 aa overlap); Q9RSD7|DR2187 PUTATIVE CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE from Deinococcus radiodurans (462 aa), FASTA scores: opt: 747, E(): 2.6e-40, (35.95% identity in 409 aa overlap); BAB50831|Q98ET6|MLL4091 CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE from Rhizobium loti (Mesorhizobium loti) (422 aa), FASTA scores: opt: 674, E(): 1.1e-35, (39.1% identity in 284 aa overlap); P30010|CFA_ECOLI|CDFA|B1661 CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIP SYNTHASE from Escherichia coli strain K12 (381 aa), FASTA scores: opt: 530, E(): 1.7e-26, (33.65% identity in 312 aa overlap); etc. Also similar to other proteins from Mycobacterium tuberculosis e.g. CMA2|Rv0503c|MTCY20G9.30c (302 aa); P96911|Rv0621|MTCY20H10 (354 aa); O50416|LPQD|Rv3390|MTV004.48 (236 aa); etc. Protein product from Mb3747 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3747 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y5D8" /db_xref="InterPro:IPR003333" /db_xref="InterPro:IPR029063" /db_xref="UniProtKB/TrEMBL:A0A1R3Y5D8" BEGIN 1 MAEILEIFTA TGQHPLKFTA YDGSTAGQDD ATLGLDLRTP RGATYLATAP GELGLARAYV 61 SGDLQAHGVH PGDPYELLKT LTERVDFKRP SARVLANVVR SIGVEHILPI APPPQEARPR 121 WRRMANGLLH SKTRDAEAIH HHYDVSNNFY EWVLGPSMTY TCAVFPNAEA SLEQAQENKY 181 RLIFEKLRLE PGDRLLDVGC GWGGMVRYAA RRGVRVIGAT LSAEQAKWGQ KAVEDEGLSD 241 LAQVRHSDYR DVAETGFDAV SSIGLTEHIG VKNYPFYFGF LKSKLRTGGL LLNHCITRHD 301 NRSTSFAGGF TDRYVFPDGE LTGSGRITTE IQQVGLEVLH EENFRHHYAM TLRDWCGNLV 361 EHWDDAVAEV GLPTAKVWGL YMAASRVAFE RNNLQLHHVL ATKVDPRGDD SLPLRPWWQP //