LOCUS       SIU02366.1               701 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 2-isopropylmalate
            synthase leua (alpha-isopropylmalate synthase) (alpha-
            ipm synthetase) (ipms) protein.
ACCESSION   LT708304-3780
PROTEIN_ID  SIU02366.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="leuA"
                     /locus_tag="BQ2027_MB3737"
                     /note="Mb3737, leuA, len: 701 aa. Similar to Rv3710, len:
                     644 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (91.7% identity in 701 aa overlap). leuA,
                     alpha-isopropylmalate synthase (EC 4.1.3.12) (see
                     citations below), equivalent to Q9CB76|LEUA|ML2324
                     2-ISOPROPYLMALATE SYNTHASE from Mycobacterium leprae (607
                     aa), FASTA scores: opt: 3291, E(): 3.7e-192, (80.7%
                     identity in 642 aa overlap). Also highly similar to many
                     e.g. P42455|LEU1_CORGL|LEUA from Corynebacterium
                     glutamicum (Brevibacterium flavum) (616 aa), FASTA scores:
                     opt: 2547, E(): 5.3e-147, (63.25% identity in 645 aa
                     overlap); O31046|LEU1_STRCO|LEUA from Streptomyces
                     coelicolor (573 aa), FASTA scores: opt: 2226, E():
                     1.5e-127, (57.8% identity in 616 aa overlap);
                     BAB49833|Q98HN3|MLR2792 from Rhizobium loti (Mesorhizobium
                     loti) (588 aa), FASTA scores: opt: 1849, E(): 1.1e-104,
                     (58.0% identity in 536 aa overlap); etc. Equivalent to
                     AAK48181 from Mycobacterium tuberculosis strain CDC1551
                     (659 aa) but shorter 15 aa. Contains PS00815 and PS00816
                     Alpha-isopropylmalate and homocitrate synthases signatures
                     1 and 2. BELONGS TO THE ALPHA-IPM SYNTHETASE / HOMOCITRATE
                     SYNTHASE FAMILY. REMARK-M.bovis-M.tuberculosis: In
                     Mycobacterium bovis, an in-frame insertion of 171 bp leads
                     to a longer product compared to its homolog in
                     Mycobacterium tuberculosis (701 aa versus 644 aa). Protein
                     product from Mb3737 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb3737 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:Q7TVV6"
                     /db_xref="InterPro:IPR000891"
                     /db_xref="InterPro:IPR002034"
                     /db_xref="InterPro:IPR005668"
                     /db_xref="InterPro:IPR013709"
                     /db_xref="InterPro:IPR013785"
                     /db_xref="InterPro:IPR036230"
                     /db_xref="InterPro:IPR039371"
                     /db_xref="UniProtKB/Swiss-Prot:Q7TVV6"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MTTSESPDAY TESFGAHTIV KPAGPPRVGQ PSWNPQRASS MPVNRYRPFA EEVEPIRLRN
       61 RTWPDRVIDR APLWCAVDLR DGNQALIDPM SPARKRRMFD LLVRMGYKEI EVGFPSASQT
      121 DFDFVREIIE QGAIPDDVTI QVLTQCRPEL IERTFQACSG AHRAIVHFYN STSILQRRVV
      181 FRANRAEVQA IATDGARKCV EQAAKYPGTQ WRFEYSPESY TGTELEYAKQ VCDAVGEVIA
      241 PTPERPIIFN LPATVEMTTP NVYADSIEWM SRNLANRESV ILSLHPHNDR GTAVAAAELG
      301 FAAGADRIEG CLFGNGERTG NVCLVTLGLN LFSRGVDPQI DFSNIDEIRR TVEYCNQLPV
      361 HERHPYGGDL VYTAFSGSHQ DAINKGLDAM KLDADAADCD VDDMLWQVPY LPIDPRDVGR
      421 TYEAVIRVNS QSGKGGVAYI MKTDHGLSLP RRLQIEFSQV IQKIAEGTAG EGGEVSPKEM
      481 WDAFAEEYLA PVRPLERIRQ HVDAADDDGG TTSITATVKI NGVETEISGS GNGPLAAFVH
      541 ALADVGFDVA VLDYYEHAMS AGDDAQAAAY VEASVTIASP AQPGEAGRHA SDPVTIASPA
      601 QPGEAGRHAS DPVTIASPAQ PGEAGRHASD PVTIASPAQP GEAGRHASDP VTIASPAQPG
      661 EAGRHASDPV TSKTVWGVGI APSITTASLR AVVSAVNRAA R
//