LOCUS SIU02366.1 701 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 2-isopropylmalate synthase leua (alpha-isopropylmalate synthase) (alpha- ipm synthetase) (ipms) protein. ACCESSION LT708304-3780 PROTEIN_ID SIU02366.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="leuA" /locus_tag="BQ2027_MB3737" /note="Mb3737, leuA, len: 701 aa. Similar to Rv3710, len: 644 aa, from Mycobacterium tuberculosis strain H37Rv, (91.7% identity in 701 aa overlap). leuA, alpha-isopropylmalate synthase (EC 4.1.3.12) (see citations below), equivalent to Q9CB76|LEUA|ML2324 2-ISOPROPYLMALATE SYNTHASE from Mycobacterium leprae (607 aa), FASTA scores: opt: 3291, E(): 3.7e-192, (80.7% identity in 642 aa overlap). Also highly similar to many e.g. P42455|LEU1_CORGL|LEUA from Corynebacterium glutamicum (Brevibacterium flavum) (616 aa), FASTA scores: opt: 2547, E(): 5.3e-147, (63.25% identity in 645 aa overlap); O31046|LEU1_STRCO|LEUA from Streptomyces coelicolor (573 aa), FASTA scores: opt: 2226, E(): 1.5e-127, (57.8% identity in 616 aa overlap); BAB49833|Q98HN3|MLR2792 from Rhizobium loti (Mesorhizobium loti) (588 aa), FASTA scores: opt: 1849, E(): 1.1e-104, (58.0% identity in 536 aa overlap); etc. Equivalent to AAK48181 from Mycobacterium tuberculosis strain CDC1551 (659 aa) but shorter 15 aa. Contains PS00815 and PS00816 Alpha-isopropylmalate and homocitrate synthases signatures 1 and 2. BELONGS TO THE ALPHA-IPM SYNTHETASE / HOMOCITRATE SYNTHASE FAMILY. REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, an in-frame insertion of 171 bp leads to a longer product compared to its homolog in Mycobacterium tuberculosis (701 aa versus 644 aa). Protein product from Mb3737 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3737 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7TVV6" /db_xref="InterPro:IPR000891" /db_xref="InterPro:IPR002034" /db_xref="InterPro:IPR005668" /db_xref="InterPro:IPR013709" /db_xref="InterPro:IPR013785" /db_xref="InterPro:IPR036230" /db_xref="InterPro:IPR039371" /db_xref="UniProtKB/Swiss-Prot:Q7TVV6" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTTSESPDAY TESFGAHTIV KPAGPPRVGQ PSWNPQRASS MPVNRYRPFA EEVEPIRLRN 61 RTWPDRVIDR APLWCAVDLR DGNQALIDPM SPARKRRMFD LLVRMGYKEI EVGFPSASQT 121 DFDFVREIIE QGAIPDDVTI QVLTQCRPEL IERTFQACSG AHRAIVHFYN STSILQRRVV 181 FRANRAEVQA IATDGARKCV EQAAKYPGTQ WRFEYSPESY TGTELEYAKQ VCDAVGEVIA 241 PTPERPIIFN LPATVEMTTP NVYADSIEWM SRNLANRESV ILSLHPHNDR GTAVAAAELG 301 FAAGADRIEG CLFGNGERTG NVCLVTLGLN LFSRGVDPQI DFSNIDEIRR TVEYCNQLPV 361 HERHPYGGDL VYTAFSGSHQ DAINKGLDAM KLDADAADCD VDDMLWQVPY LPIDPRDVGR 421 TYEAVIRVNS QSGKGGVAYI MKTDHGLSLP RRLQIEFSQV IQKIAEGTAG EGGEVSPKEM 481 WDAFAEEYLA PVRPLERIRQ HVDAADDDGG TTSITATVKI NGVETEISGS GNGPLAAFVH 541 ALADVGFDVA VLDYYEHAMS AGDDAQAAAY VEASVTIASP AQPGEAGRHA SDPVTIASPA 601 QPGEAGRHAS DPVTIASPAQ PGEAGRHASD PVTIASPAQP GEAGRHASDP VTIASPAQPG 661 EAGRHASDPV TSKTVWGVGI APSITTASLR AVVSAVNRAA R //