LOCUS       SIU02359.1               432 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 GLUTAMATE-
            -CYSTEINE LIGASE GSHA (GAMMA-GLUTAMYLCYSTEINE SYNTHETASE)
            (GAMMA-ECS) (GCS) (GAMMA-GLUTAMYL-L-CYSTEINE SYNTHETASE)
            protein.
ACCESSION   LT708304-3773
PROTEIN_ID  SIU02359.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="gshA"
                     /locus_tag="BQ2027_MB3730C"
                     /note="Mb3730c, gshA, len: 432 aa. Equivalent to Rv3704c,
                     len: 432 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 432 aa overlap). Possible gshA,
                     glutamate--cysteine ligase (EC 6.3.2.2), similar to many
                     e.g. Q9A2Z2|CC3414 GLUTAMATE--CYSTEINE LIGASE from
                     Caulobacter crescentus (453 aa), FASTA scores: opt: 404,
                     E(): 5.9e-17, (30.45% identity in 312 aa overlap);
                     Q9SEH0|GSH1 GAMMA-GLUTAMYLCYSTEINYL SYNTHETASE PRECURSOR
                     from Pisum sativum (Garden pea) (499 aa), FASTA scores:
                     opt: 400, E(): 1.1e-16, (26.4% identity in 439 aa
                     overlap); Q9RH09|GSH GAMMA-GLUTAMYLCYSTEINE SYNTHETASE
                     from Zymomonas mobilis (462 aa), FASTA scores: opt: 397,
                     E(): 1.6e-16, (28.95% identity in 304 aa overlap);
                     P46309|GSH1_ARATH|GSH1|AT4G23100|F7H19.290
                     GLUTAMATE--CYSTEINE LIGASE from Arabidopsis thaliana
                     (Mouse-ear cress) (522 aa), FASTA scores: opt: 395, E():
                     2.3e-16, (27.25% identity in 385 aa overlap); etc. But
                     note that this putative protein is also similar to
                     Q9JMV4|GSHA PUTATIVE GLUTATHIONE SYNTHETASE (FRAGMENT)
                     from Bradyrhizobium japonicum (460 aa), FASTA scores: opt:
                     498, E(): 1.3e-22, (33.35% identity in 333 aa overlap) (no
                     significant publications found (August 2001). Mb3730c
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3Y503"
                     /db_xref="InterPro:IPR006336"
                     /db_xref="InterPro:IPR014746"
                     /db_xref="InterPro:IPR017809"
                     /db_xref="InterPro:IPR035434"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y503"
BEGIN
        1 MTLAAMTAAA SQLDNAAPDD VEITDSSAAA EYIADGCLVD GPLGRVGLEM EAHCFDPADP
       61 FRRPSWEEIT EVLEWLSPLP GGSVVSVEPG GAVELSGPPA DGVLAAIGAM TRDQAVLRSA
      121 LANAGLGLVF LGADPLRSPV RVNPGARYRA MEQFFAASHS GVPGAAMMTS TAAIQVNLDA
      181 GPQEGWAERV RLAHALGPTM IAIAANSPML GGRFSGWQST RQRVWGQMDS ARCGPILGAS
      241 GDHPGIDWAK YALKAPVMMV RSPDTQDTRA VTDYVPFTDW VDGRVLLDGR RATVADLVYH
      301 LTTLFPPVRP RQWLEIRYLD SVPDEVWPAV VFTLVTLLDD PVAADLAVDA VEPVATAWDT
      361 AARIGLADRR LYLAANRCLA IAARRVPTEL IGAMQRLVDH VDRGVCPADD FSDRVIAGGI
      421 ASAVTGMMHG AS
//