LOCUS SIU02359.1 432 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 GLUTAMATE- -CYSTEINE LIGASE GSHA (GAMMA-GLUTAMYLCYSTEINE SYNTHETASE) (GAMMA-ECS) (GCS) (GAMMA-GLUTAMYL-L-CYSTEINE SYNTHETASE) protein. ACCESSION LT708304-3773 PROTEIN_ID SIU02359.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="gshA" /locus_tag="BQ2027_MB3730C" /note="Mb3730c, gshA, len: 432 aa. Equivalent to Rv3704c, len: 432 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 432 aa overlap). Possible gshA, glutamate--cysteine ligase (EC 6.3.2.2), similar to many e.g. Q9A2Z2|CC3414 GLUTAMATE--CYSTEINE LIGASE from Caulobacter crescentus (453 aa), FASTA scores: opt: 404, E(): 5.9e-17, (30.45% identity in 312 aa overlap); Q9SEH0|GSH1 GAMMA-GLUTAMYLCYSTEINYL SYNTHETASE PRECURSOR from Pisum sativum (Garden pea) (499 aa), FASTA scores: opt: 400, E(): 1.1e-16, (26.4% identity in 439 aa overlap); Q9RH09|GSH GAMMA-GLUTAMYLCYSTEINE SYNTHETASE from Zymomonas mobilis (462 aa), FASTA scores: opt: 397, E(): 1.6e-16, (28.95% identity in 304 aa overlap); P46309|GSH1_ARATH|GSH1|AT4G23100|F7H19.290 GLUTAMATE--CYSTEINE LIGASE from Arabidopsis thaliana (Mouse-ear cress) (522 aa), FASTA scores: opt: 395, E(): 2.3e-16, (27.25% identity in 385 aa overlap); etc. But note that this putative protein is also similar to Q9JMV4|GSHA PUTATIVE GLUTATHIONE SYNTHETASE (FRAGMENT) from Bradyrhizobium japonicum (460 aa), FASTA scores: opt: 498, E(): 1.3e-22, (33.35% identity in 333 aa overlap) (no significant publications found (August 2001). Mb3730c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y503" /db_xref="InterPro:IPR006336" /db_xref="InterPro:IPR014746" /db_xref="InterPro:IPR017809" /db_xref="InterPro:IPR035434" /db_xref="UniProtKB/TrEMBL:A0A1R3Y503" BEGIN 1 MTLAAMTAAA SQLDNAAPDD VEITDSSAAA EYIADGCLVD GPLGRVGLEM EAHCFDPADP 61 FRRPSWEEIT EVLEWLSPLP GGSVVSVEPG GAVELSGPPA DGVLAAIGAM TRDQAVLRSA 121 LANAGLGLVF LGADPLRSPV RVNPGARYRA MEQFFAASHS GVPGAAMMTS TAAIQVNLDA 181 GPQEGWAERV RLAHALGPTM IAIAANSPML GGRFSGWQST RQRVWGQMDS ARCGPILGAS 241 GDHPGIDWAK YALKAPVMMV RSPDTQDTRA VTDYVPFTDW VDGRVLLDGR RATVADLVYH 301 LTTLFPPVRP RQWLEIRYLD SVPDEVWPAV VFTLVTLLDD PVAADLAVDA VEPVATAWDT 361 AARIGLADRR LYLAANRCLA IAARRVPTEL IGAMQRLVDH VDRGVCPADD FSDRVIAGGI 421 ASAVTGMMHG AS //