LOCUS SIU02337.1 346 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
LYASE protein.
ACCESSION LT708304-3751
PROTEIN_ID SIU02337.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/locus_tag="BQ2027_MB3709"
/note="Mb3709, -, len: 346 aa. Equivalent to Rv3684, len:
346 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 346 aa overlap). Probable lyase (EC
4.-.-.-), and more specifically a cysteine synthase (EC
4.2.99.8), highly similar to many lyases e.g.
Q9K3N2|SCG20A.08c PUTATIVE LYASE from Streptomyces
coelicolor (374 aa), FASTA scores: opt: 1469, E():
3.7e-85, (63.35% identity in 341 aa overlap) (shorter 31
aa at N-terminus); Q9KT44|VC1061 CYSTEINE SYNTHASE (EC
4.2.99.8)/CYSTATHIONINE BETA-SYNTHASE FAMILY PROTEIN from
Vibrio cholerae (355 aa), FASTA scores: opt: 1366, E():
1.1e-78, (63.25% identity in 321 aa overlap);
Q9I4R3|PA1061 HYPOTHETICAL PROTEIN from Pseudomonas
aeruginosa (365 aa), FASTA scores: opt: 1311, E():
3.2e-75, (59.8% identity in 341 aa overlap); Q9PH18|XF0128
CYSTEINE SYNTHASE from Xylella fastidiosa (390 aa), FASTA
scores: opt: 1288, E(): 9.5e-74, (58.55% identity in 333
aa overlap) (shorter 34 aa at N-terminus);
P55708|Y4XP_RHISN PUTATIVE CYSTEINE SYNTHASE from
Rhizobium sp. strain NGR234 plasmid sym pNGR234a (336 aa),
FASTA scores: opt: 376, E(): 2.1e-16, (29.2% identity in
315 aa overlap); etc. Equivalent to AAK48153 from
Mycobacterium tuberculosis strain CDC1551 (368 aa) but
shorter 22 aa. Protein product from Mb3709 detected using
shotgun mass spectrometry and SWATH mass spectrometry.
Mb3709 found to be expressed during exponential growth in
Sauton's minimal media by RNA-sequencing."
/db_xref="GOA:A0A1R3Y4W2"
/db_xref="InterPro:IPR001926"
/db_xref="InterPro:IPR036052"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y4W2"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MIEADARRSA DTHLLRYPLP AAWCTDVDVE LYLKDETTHI TGSLKHRLAR SLFLYALCNG
61 WINENTTVVE ASSGSTAVSE AYFAALLGLP FIAVMPAATS ASKIALIESQ GGRCHFVQNS
121 SQVYAEAERV AKETGGHYLD QFTNAERATD WRGNNNIAES IYVQMREEKH PTPEWIVVGA
181 GTGGTSATIG RYIRYRRHAT RLCVVDPENS AFFPAYSEGR YDIVMPTSSR IEGIGRPRVE
241 PSFLPGVVDR MVAVPDAASI AAARHVSAVL GRRVGPSTGT NLWGAFGLLA EMVKQGRSGS
301 VVTLLADSGD RYADTYFSDE WVSAQGLDPA GPAAALVEFE RSCRWT
//