LOCUS       SIU02326.1               245 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ENDONUCLEASE III NTH (DNA-(APURINIC OR APYRIMIDINIC SITE)
            LYASE) (AP LYASE) (AP ENDONUCLEASE CLASS I) (ENDODEOXYRIBONUCLEASE
            (APURINIC OR APYRIMIDINIC)) (DEOXYRIBONUCLEASE (APURINIC
            OR APYRIMIDINIC)) protein.
ACCESSION   LT708304-3740
PROTEIN_ID  SIU02326.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="nth"
                     /locus_tag="BQ2027_MB3698C"
                     /note="Mb3698c, nth, len: 245 aa. Equivalent to Rv3674c,
                     len: 245 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 245 aa overlap). Probable nth,
                     endonuclease III (EC 4.2.99.18), equivalent to
                     Q9CB92|NTH|ML2301 PUTATIVE ENDONUCLEASE III from
                     Mycobacterium leprae (272 aa), FASTA scores: opt: 1363,
                     E(): 3.6e-81, (89.4% identity in 226 aa overlap). Also
                     similar to many e.g. Q9XA44|SCH17.03c from Streptomyces
                     coelicolor (250 aa), FASTA scores: opt: 937, E(): 2.2e-55,
                     (61.65% identity in 219 aa overlap); P46303|UVEN_MICLU
                     from Micrococcus luteus (Micrococcus lysodeikticus) (279
                     aa), FASTA scores: opt: 899, E(): 8.1e-53, (58.45%
                     identity in 248 aa overlap); P73715|END3_SYNY3|NTH|SLR1822
                     from Synechocystis sp. strain PCC 6803 (219 aa), FASTA
                     scores: opt: 684, E(): 1.7e-38, (52.2% identity in 203 aa
                     overlap); P39788|END3_BACSU|NTH|JOOB from Bacillus
                     subtilis (219 aa), FASTA scores: opt: 552, E(): 1.2e-29,
                     (43.3% identity in 194 aa overlap); etc. Equivalent to
                     AAK48142 from Mycobacterium tuberculosis strain CDC1551
                     (262 aa) but shorter 17 aa. Contains PS00764 Endonuclease
                     III iron-sulfur binding region signature, and PS01155
                     Endonuclease III family signature. BELONGS TO THE NTH/MUTY
                     FAMILY. COFACTOR: BINDS A 4FE-4S CLUSTER WHICH IS NOT
                     IMPORTANT FOR THE CATALYTIC ACTIVITY, BUT WHICH IS
                     PROBABLY INVOLVED IN THE PROPER POSITIONING OF THE ENZYME
                     ALONG THE DNA STRAND (BY SIMILARITY). N-terminus extended
                     since first submission (previously 226 aa). Protein
                     product from Mb3698c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb3698c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:P63541"
                     /db_xref="InterPro:IPR000445"
                     /db_xref="InterPro:IPR003265"
                     /db_xref="InterPro:IPR003651"
                     /db_xref="InterPro:IPR004035"
                     /db_xref="InterPro:IPR004036"
                     /db_xref="InterPro:IPR005759"
                     /db_xref="InterPro:IPR011257"
                     /db_xref="InterPro:IPR023170"
                     /db_xref="UniProtKB/Swiss-Prot:P63541"
BEGIN
        1 MPGRWSAETR LALVRRARRM NRALAQAFPH VYCELDFTTP LELAVATILS AQSTDKRVNL
       61 TTPALFARYR TARDYAQADR TELESLIRPT GFYRNKAASL IGLGQALVER FGGEVPATMD
      121 KLVTLPGVGR KTANVILGNA FGIPGITVDT HFGRLVRRWR WTTAEDPVKV EQAVGELIER
      181 KEWTLLSHRV IFHGRRVCHA RRPACGVCVL AKDCPSFGLG PTEPLLAAPL VQGPETDHLL
      241 ALAGL
//