LOCUS       SIU02323.1               397 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 membrane-
            associated serine protease protein.
ACCESSION   LT708304-3737
PROTEIN_ID  SIU02323.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /locus_tag="BQ2027_MB3695C"
                     /note="Mb3695c, -, len: 397 aa. Equivalent to Rv3671c,
                     len: 397 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.7% identity in 397 aa overlap). Possible serine
                     protease membrane protein (EC 3.4.21.-), equivalent to
                     Q9CB95|ML2298 PUTATIVE MEMBRANE-ASSOCIATED SERINE PROTEASE
                     from Mycobacterium leprae (401 aa), FASTA scores: opt:
                     2061, E(): 2.3e-108, (80.9% identity in 398 aa overlap).
                     Also similar to many serine proteases, but generally with
                     extended N-terminus, e.g. Q9X932|SCH5.30c PUTATIVE SERINE
                     PROTEASE (FRAGMENT) from Streptomyces coelicolor (385 aa),
                     FASTA scores: opt: 835, E(): 1.2e-39, (39.9% identity in
                     386 aa overlap); Q9Z6T0|DEGP_CHLPN|HTRA|CPN0979|CP0877
                     PROBABLE SERINE PROTEASE DO-LIKE PRECURSOR from Chlamydia
                     pneumoniae (Chlamydophila pneumoniae) (488 aa), FASTA
                     scores: opt: 285, E(): 1e-08, (29.05% identity in 296 aa
                     overlap); P73354|HTRA|SLR1204 SERINE PROTEASE from
                     Synechocystis sp. strain PCC 6803 (452 aa), FASTA scores:
                     opt: 284, E(): 1.1e-08, (29.55% identity in 308 aa
                     overlap); Q9RWC4|DR0745 PERIPLASMIC SERINE PROTEASE,
                     HTRA/DEGQ/DEGS FAMILY from Deinococcus radiodurans (366
                     aa), FASTA scores: opt: 271, E(): 4.9e-08, (35.45%
                     identity in 206 aa overlap); etc. Also similar, but longer
                     114 aa at the N-terminus, to Q9S2P8|SC5F7.13 PUTATIVE
                     PEPTIDASE from Streptomyces coelicolor (282 aa), FASTA
                     scores: opt: 594, E(): 3.1e-26, (38.95% identity in 285 aa
                     overlap). And similar, but longer 146 aa at the
                     N-terminus, to O07175|PEPA|Rv0125|MTCI418B.07 from
                     Mycobacterium tuberculosis (355 aa), FASTA scores: opt:
                     295, E(): 2.2e-09, (29.55% identity in 254 aa overlap);
                     and Q9CCY9|ML2659 PROBABLE SECRETED SERINE PROTEASE from
                     Mycobacterium leprae FASTA scores: opt: 286, E(): 6.9e-09,
                     (30.6% identity in 255 aa overlap). Contains PS00135
                     Serine proteases, trypsin family, serine active site.
                     Protein product from Mb3695c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb3695c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3Y4V3"
                     /db_xref="InterPro:IPR001940"
                     /db_xref="InterPro:IPR003825"
                     /db_xref="InterPro:IPR009003"
                     /db_xref="InterPro:IPR033116"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y4V3"
BEGIN
        1 MTPSQWLDIA VLAVAFIAAI SGWRAGALGS MLSFGGVLLG ATAGVLLAPH IVSQISAPRA
       61 KLFAALFLIL ALVVVGEVAG VVLGRAVRGA IRNRPIRLID SVIGVGVQLV VVLTAAWLLA
      121 MPLTQSKEQP ELAAAVKGSR VLARVNEAAP TWLKTVPKRL SALLNTSGLP AVLEPFSRTP
      181 VIPVASPDPA LVNNPVVAAT EPSVVKIRSL APRCQKVLEG TGFVISPDRV MTNAHVVAGS
      241 NNVTVYAGDK PFEATVVSYD PSVDVAILAV PHLPPPPLVF AAEPAKTGAD VVVLGYPGGG
      301 NFTATPARIR EAIRLSGPDI YGDPEPVTRD VYTIRADVEQ GDSGGPLIDL NGQVLGVVFG
      361 AAVDDAETGF VLTAGEVAGQ LAKIGATQPV GTGACVS
//