LOCUS SIU02323.1 397 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 membrane- associated serine protease protein. ACCESSION LT708304-3737 PROTEIN_ID SIU02323.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3695C" /note="Mb3695c, -, len: 397 aa. Equivalent to Rv3671c, len: 397 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 397 aa overlap). Possible serine protease membrane protein (EC 3.4.21.-), equivalent to Q9CB95|ML2298 PUTATIVE MEMBRANE-ASSOCIATED SERINE PROTEASE from Mycobacterium leprae (401 aa), FASTA scores: opt: 2061, E(): 2.3e-108, (80.9% identity in 398 aa overlap). Also similar to many serine proteases, but generally with extended N-terminus, e.g. Q9X932|SCH5.30c PUTATIVE SERINE PROTEASE (FRAGMENT) from Streptomyces coelicolor (385 aa), FASTA scores: opt: 835, E(): 1.2e-39, (39.9% identity in 386 aa overlap); Q9Z6T0|DEGP_CHLPN|HTRA|CPN0979|CP0877 PROBABLE SERINE PROTEASE DO-LIKE PRECURSOR from Chlamydia pneumoniae (Chlamydophila pneumoniae) (488 aa), FASTA scores: opt: 285, E(): 1e-08, (29.05% identity in 296 aa overlap); P73354|HTRA|SLR1204 SERINE PROTEASE from Synechocystis sp. strain PCC 6803 (452 aa), FASTA scores: opt: 284, E(): 1.1e-08, (29.55% identity in 308 aa overlap); Q9RWC4|DR0745 PERIPLASMIC SERINE PROTEASE, HTRA/DEGQ/DEGS FAMILY from Deinococcus radiodurans (366 aa), FASTA scores: opt: 271, E(): 4.9e-08, (35.45% identity in 206 aa overlap); etc. Also similar, but longer 114 aa at the N-terminus, to Q9S2P8|SC5F7.13 PUTATIVE PEPTIDASE from Streptomyces coelicolor (282 aa), FASTA scores: opt: 594, E(): 3.1e-26, (38.95% identity in 285 aa overlap). And similar, but longer 146 aa at the N-terminus, to O07175|PEPA|Rv0125|MTCI418B.07 from Mycobacterium tuberculosis (355 aa), FASTA scores: opt: 295, E(): 2.2e-09, (29.55% identity in 254 aa overlap); and Q9CCY9|ML2659 PROBABLE SECRETED SERINE PROTEASE from Mycobacterium leprae FASTA scores: opt: 286, E(): 6.9e-09, (30.6% identity in 255 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Protein product from Mb3695c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3695c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y4V3" /db_xref="InterPro:IPR001940" /db_xref="InterPro:IPR003825" /db_xref="InterPro:IPR009003" /db_xref="InterPro:IPR033116" /db_xref="UniProtKB/TrEMBL:A0A1R3Y4V3" BEGIN 1 MTPSQWLDIA VLAVAFIAAI SGWRAGALGS MLSFGGVLLG ATAGVLLAPH IVSQISAPRA 61 KLFAALFLIL ALVVVGEVAG VVLGRAVRGA IRNRPIRLID SVIGVGVQLV VVLTAAWLLA 121 MPLTQSKEQP ELAAAVKGSR VLARVNEAAP TWLKTVPKRL SALLNTSGLP AVLEPFSRTP 181 VIPVASPDPA LVNNPVVAAT EPSVVKIRSL APRCQKVLEG TGFVISPDRV MTNAHVVAGS 241 NNVTVYAGDK PFEATVVSYD PSVDVAILAV PHLPPPPLVF AAEPAKTGAD VVVLGYPGGG 301 NFTATPARIR EAIRLSGPDI YGDPEPVTRD VYTIRADVEQ GDSGGPLIDL NGQVLGVVFG 361 AAVDDAETGF VLTAGEVAGQ LAKIGATQPV GTGACVS //