LOCUS SIU02286.1 314 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 UDP-
GLUCOSE 4-EPIMERASE GALE1 (GALACTOWALDENASE) (UDP-GALACTOSE
4-EPIMERASE) (URIDINE DIPHOSPHATE GALACTOSE 4-EPIMERASE)
(URIDINE DIPHOSPHO-GALACTOSE 4-EPIMERASE) protein.
ACCESSION LT708304-3700
PROTEIN_ID SIU02286.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="galE1"
/locus_tag="BQ2027_MB3658C"
/note="Mb3658c, galE1, len: 314 aa. Equivalent to Rv3634c,
len: 314 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 314 aa overlap). galE1, UDP-glucose
4-epimerase (EC 5.1.3.2) (see citations below), equivalent
to O69544|ML0204|RMLB2|MLCB2548.27c PUTATIVE SUGAR
DEHYDRATASE (PUTATIVE SUGAR-NUCLEOTIDE DEHYDRATASE) from
Mycobacterium leprae (319 aa), FASTA scores: opt: 1798,
E(): 8.2e-100, (86.4% identity in 309 aa overlap). Also
similar to other UDP-GLUCOSE 4-EPIMERASES e.g.
Q9WYX9|TM0509 from Thermotoga maritima (309 aa) FASTA
scores: opt: 877, E(): 4.8e-45, (45.8% identity in 308 aa
overlap); Q57664|GALE_METJA|MJ0211 from Methanococcus
jannaschii (305 aa), FASTA scores: opt: 792, E(): 5.4e-40,
(42.05% identity in 309 aa overlap); Q9K6S7|BH3649 from
Bacillus halodurans (311 aa), FASTA scores: opt: 723, E():
7e-36, (40.5% identity in 316 aa overlap);
Q9HSV1|GALE2|VNG0063G from Halobacterium sp. strain NRC-1
(328 aa), FASTA scores: opt: 597, E(): 2.3e-28, (36.35%
identity in 322 aa overlap); etc. Contains short-chain
alcohol dehydrogenase family signature (PS00061) but this
maynot be significant. BELONGS TO THE SUGAR EPIMERASE
FAMILY. Note that previously known as rmlB2, a
DTDP-glucose 4,6-dehydratase (EC 4.2.1.46) (see third
citation). Protein product from Mb3658c detected using
shotgun mass spectrometry and SWATH mass spectrometry.
Mb3658c found to be expressed during exponential growth in
Sauton's minimal media by RNA-sequencing."
/db_xref="InterPro:IPR016040"
/db_xref="InterPro:IPR036291"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y594"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MRALVTGAAG FIGSTLVDRL LADGHSVVGL DNFATGRATN LEHLADNSAH VFVEADIVTA
61 DLHAILEQHR PEVVFHLAAQ IDVRRSVADP QFDAAVNVIG TVRLAEAARQ TGVRKIVHTS
121 SGGSIYGTPP EYPTPETAPT DPASPYAAGK VAGEIYLNTF RHLYGLDCSH IAPANVYGPR
181 QDPHGEAGVV AIFAQALLSG KPTRVFGDGT NTRDYVFVDD VVDAFVRVSA DVGGGLRFNI
241 GTGKETSDRQ LHSAVAAAVG GPDDPEFHPP RLGDLKRSCL DIGLAERVLG WRPQIELADG
301 VRRTVEYFRH KHTD
//