LOCUS SIU02280.1 162 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 INORGANIC
PYROPHOSPHATASE PPA (PYROPHOSPHATE PHOSPHO-HYDROLASE)
(PPASE) (INORGANIC DIPHOSPHATASE) (DIPHOSPHATE PHOSPHO-
HYDROLASE) protein.
ACCESSION LT708304-3694
PROTEIN_ID SIU02280.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="ppa"
/locus_tag="BQ2027_MB3652"
/note="Mb3652, ppa, len: 162 aa. Equivalent to Rv3628,
len: 162 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 162 aa overlap). ppa, inorganic
pyrophosphatase (EC 3.6.1.1) (see first citation),
identical to O69540|IPYR_MYCLEPPA|ML0210|MLCB2548.21
INORGANIC PYROPHOSPHATASE from Mycobacterium leprae (162
aa) FASTA scores: opt: 1018, E(): 1.3e-59, (89.5% identity
in 162 aa overlap). Also highly similar to many bacterial
pyrophosphatases e.g. Q9X8I9|IPYR_STRCO|PPA|SCE9.16 from
Streptomyces coelicolor (163 aa), FASTA scores: opt: 773,
E(): 1.3e-43, (67.5% identity in 163 aa overlap);
O05545|IPYR_GLUOX|PPA from Gluconobacter oxydans
(Gluconobacter suboxydans) (176 aa), FASTA scores: opt:
553, E(): 3.2e-29, (53.8% identity in 145 aa overlap);
P77992|IPYR_THELI|PPA from Thermococcus litoralis (176 aa)
FASTA scores: opt: 537, E(): 3.5e-28, (49.35% identity in
152 aa overlap); P50308|IPYR_SULAC|PPA from Sulfolobus
acidocaldarius (173 aa), FASTA scores: opt: 518, E():
6e-27, (45.3% identity in 159 aa overlap); etc. BELONGS TO
THE PPASE FAMILY. COFACTOR: REQUIRES THE PRESENCE OF
DIVALENT METAL CATION. MAGNESIUM CONFERS THE HIGHEST
ACTIVITY. BINDS 4 DIVALENT CATIONS PER SUBUNIT (BY
SIMILARITY). Protein product from Mb3652 detected using
shotgun mass spectrometry and SWATH mass spectrometry.
Mb3652 found to be expressed during exponential growth in
Sauton's minimal media by RNA-sequencing."
/db_xref="GOA:P65747"
/db_xref="InterPro:IPR008162"
/db_xref="InterPro:IPR036649"
/db_xref="UniProtKB/Swiss-Prot:P65747"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MQFDVTIEIP KGQRNKYEVD HETGRVRLDR YLYTPMAYPT DYGFIEDTLG DDGDPLDALV
61 LLPQPVFPGV LVAARPVGMF RMVDEHGGDD KVLCVPAGDP RWDHVQDIGD VPAFELDAIK
121 HFFVHYKDLE PGKFVKAADW VDRAEAEAEV QRSVERFKAG TH
//