LOCUS SIU02277.1 323 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE CELL CYCLE PROTEIN MESJ protein. ACCESSION LT708304-3691 PROTEIN_ID SIU02277.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mesJ" /locus_tag="BQ2027_MB3649C" /note="Mb3649c, mesJ, len: 323 aa. Equivalent to Rv3625c, len: 323 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 323 aa overlap). Possible mesJ, cell cycle protein, equivalent to O69538|Y0C5_MYCLE|ML0213|MLCB2548.18c HYPOTHETICAL 34.1 KDA PROTEIN from Mycobacterium leprae (323 aa) FASTA scores: opt: 1592, E(): 9e-92, (78.0% identity in 327 aa overlap). Similar to bacterial hypothetical proteins Q9X8I6|SCE9.13c from Streptomyces coelicolor (352 aa) FASTA scores: opt: 705, E(): 1.4e-36, (47.85% identity in 305 aa overlap); and Q9HXZ3|PA3638 from Pseudomonas aeruginosa (442 aa), FASTA scores: opt: 382, E(): 2e-16, (40.6% identity in 271 aa overlap). But also similar (or with similarity) to bacterial cell cycle proteins (MESJ) e.g. Q9KPX0|VC2242 MESJ PROTEIN from Vibrio cholerae (440 aa), FASTA scores: opt: 363, E(): 3e-15, (34.8% identity in 253 aa overlap); Q9RV23|DR1207 (600 aa) CELL CYCLE PROTEIN MESJ (PUTATIVE/CYTOSINE DEAMINASE-RELATED PROTEIN) from Deinococcus radiodurans (600 aa), FASTA scores: opt: 310, E(): 7.6e-12, (36.6% identity in 265 aa overlap) (similar only at the N-terminal end); Q9PFJ8|XF0659 CELL CYCLE PROTEIN from Xylella fastidiosa (437 aa), FASTA scores: opt: 301, E(): 2.1e-11, (35.05% identity in 271 aa overlap); P52097|MESJ_ECOLI|B0188 PUTATIVE CELL CYCLE PROTEIN MESJ from Escherichia coli strain K12(432 aa) FASTA scores: opt: 299, E(): 2.8e-11, (34.65% identity in 277 aa overlap); etc. BELONGS TO THE UPF0072 (MESJ/YCF62) FAMILY. Protein product from Mb3649c detected using SWATH mass spectrometry. Mb3649c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P67152" /db_xref="InterPro:IPR011063" /db_xref="InterPro:IPR012094" /db_xref="InterPro:IPR012795" /db_xref="InterPro:IPR014729" /db_xref="InterPro:IPR015262" /db_xref="UniProtKB/Swiss-Prot:P67152" BEGIN 1 MDRQSAVAQL RAAAEQFARV HLDACDRWSV GLSGGPDSLA LTAVAARLWP TTALIVDHGL 61 QPGSATVAET ARIQAISLGC VDARVLCVQV GAAGGREAAA RSARYSALEE HRDGPVLLAH 121 TLDDQAETVL LGLGRGSGAR SIAGMRPYDP PWCRPLLGVR RSVTHAACRE LGLTAWQDPH 181 NTDRRFTRTR LRTEVLPLLE DVLGGGVAEA LARTATALRE DTDLIDTIAA QALPGAAVAG 241 SRGQELSTSA LTALPDAVRR RVIRGWLLAG GATGLTDRQI RGVDRLVTAW RGQGGVAVGS 301 TLRGQRLVAG RRDGVLVLRR EPV //