LOCUS       SIU02239.1               159 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            2C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE ISPF
            (MECPS) protein.
ACCESSION   LT708304-3653
PROTEIN_ID  SIU02239.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="ispF"
                     /locus_tag="BQ2027_MB3612C"
                     /note="Mb3612c, ispF, len: 159 aa. Equivalent to Rv3581c,
                     len: 159 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 159 aa overlap). Probable ispF,
                     2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC
                     not defined), equivalent to Q9CCW5|ML0322 PUTATIVE
                     2-C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE from
                     Mycobacterium leprae (158 aa), FASTA scores: opt: 830,
                     E(): 2.9e-47, (79.1% identity in 158 aa overlap). Also
                     highly similar to others e.g. Q9L0Q7|ISPF_STRCO|SCD8A.07
                     from Streptomyces coelicolor (170 aa), FASTA scores: opt:
                     585, E(): 2.9e-31, (56.5% identity in 154 aa overlap);
                     Q9PDT5|ISPF_XYLFA|XF1294 from Xylella fastidiosa (176 aa),
                     FASTA scores: opt: 398, E(): 4.6e-19, (44.9% identity in
                     156 aa overlap); Q08113|ISDF_RHOCA|ISPDF from Rhodobacter
                     capsulatus (Rhodopseudomonas capsulata) (379 aa), FASTA
                     scores: opt: 387, E(): 4.5e-18, (42.85% identity in 154 aa
                     overlap) (only similar with C-terminal end of this
                     bifunctional protein ISPD and ISPF); Q06756|ISPF_BACSU
                     from Bacillus subtilis (158 aa), FASTA scores: opt: 367,
                     E(): 4.5e-17, (41.2% identity in 153 aa overlap); etc.
                     BELONGS TO THE ISPF FAMILY. Protein product from Mb3612c
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb3612c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P65184"
                     /db_xref="InterPro:IPR003526"
                     /db_xref="InterPro:IPR020555"
                     /db_xref="InterPro:IPR036571"
                     /db_xref="UniProtKB/Swiss-Prot:P65184"
BEGIN
        1 MNQLPRVGLG TDVHPIEPGR PCWLVGLLFP SADGCAGHSD GDVAVHALCD AVLSAAGLGD
       61 IGEVFGVDDP RWQGVSGADM LRHVVVLITQ HGYRVGNAVV QVIGNRPKIG WRRLEAQAVL
      121 SRLLNAPVSV SATTTDGLGL TGRGEGLAAI ATALVVSLR
//