LOCUS SIU02239.1 159 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE 2C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE ISPF (MECPS) protein. ACCESSION LT708304-3653 PROTEIN_ID SIU02239.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ispF" /locus_tag="BQ2027_MB3612C" /note="Mb3612c, ispF, len: 159 aa. Equivalent to Rv3581c, len: 159 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 159 aa overlap). Probable ispF, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC not defined), equivalent to Q9CCW5|ML0322 PUTATIVE 2-C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE from Mycobacterium leprae (158 aa), FASTA scores: opt: 830, E(): 2.9e-47, (79.1% identity in 158 aa overlap). Also highly similar to others e.g. Q9L0Q7|ISPF_STRCO|SCD8A.07 from Streptomyces coelicolor (170 aa), FASTA scores: opt: 585, E(): 2.9e-31, (56.5% identity in 154 aa overlap); Q9PDT5|ISPF_XYLFA|XF1294 from Xylella fastidiosa (176 aa), FASTA scores: opt: 398, E(): 4.6e-19, (44.9% identity in 156 aa overlap); Q08113|ISDF_RHOCA|ISPDF from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (379 aa), FASTA scores: opt: 387, E(): 4.5e-18, (42.85% identity in 154 aa overlap) (only similar with C-terminal end of this bifunctional protein ISPD and ISPF); Q06756|ISPF_BACSU from Bacillus subtilis (158 aa), FASTA scores: opt: 367, E(): 4.5e-17, (41.2% identity in 153 aa overlap); etc. BELONGS TO THE ISPF FAMILY. Protein product from Mb3612c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3612c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65184" /db_xref="InterPro:IPR003526" /db_xref="InterPro:IPR020555" /db_xref="InterPro:IPR036571" /db_xref="UniProtKB/Swiss-Prot:P65184" BEGIN 1 MNQLPRVGLG TDVHPIEPGR PCWLVGLLFP SADGCAGHSD GDVAVHALCD AVLSAAGLGD 61 IGEVFGVDDP RWQGVSGADM LRHVVVLITQ HGYRVGNAVV QVIGNRPKIG WRRLEAQAVL 121 SRLLNAPVSV SATTTDGLGL TGRGEGLAAI ATALVVSLR //